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. 1966 Oct;11(4):361–368.

Heterogeneity of cold haemagglutinins

O Tönder, M Harboe
PMCID: PMC1423830  PMID: 5924621

Abstract

All of seven sera with high titre cold agglutinins agglutinated rabbit red cells at 4° to similar titres as were recorded with human red cells. At 20° all the sera showed almost optimal activity against rabbit red cells, while the activity against human red cells was very weak. Two of the sera agglutinated rabbit red cells as strongly at 37° as at lower temperatures.

The results of absorption and elution experiments indicated that the same agglutinin molecules were responsible for the agglutination of human red cells at 4° and of rabbit red cells at 37°. Apparently the reaction with rabbit red cells was more specific than the reaction with human red cells. The variation in thermal amplitude observed when testing cold agglutinins with rabbit red cells may be an expression of variation in specificity even when the different cold agglutinins appear to have identical specificity, anti-I, in tests with human red cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADINOLFI M., POLLEY M. J., HUNTER D. A., MOLLISON P. L. Classification of blood-group antibodies as beta2M or gamma globulin. Immunology. 1962 Sep;5:566–579. [PMC free article] [PubMed] [Google Scholar]
  2. COSTEA N., YAKULIS V., HELLER P. EXPERIMENTAL PRODUCTION OF COLD AGGLUTININS IN RABBITS. Blood. 1965 Sep;26:323–340. [PubMed] [Google Scholar]
  3. Dacie J. V. Hemolytic anemia. Ann N Y Acad Sci. 1965 Jun 30;124(2):415–421. doi: 10.1111/j.1749-6632.1965.tb18974.x. [DOI] [PubMed] [Google Scholar]
  4. FUDENBERG H. H., KUNKEL H. G. Physical properties of the red cell agglutinins in acquired hemolytic anemia. J Exp Med. 1957 Nov 1;106(5):689–702. doi: 10.1084/jem.106.5.689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HINZ C. F., Jr SEROLOGIC AND PHYSICOCHEMICAL CHARACTERIZATION OF DONATH- LANDSTEINER ANTIBODIES FROM SIX PATIENTS. Blood. 1963 Nov;22:600–605. [PubMed] [Google Scholar]
  6. Harboe M., van Furth R., Schubothe H., Lind K., Evans R. S. Exclusive occurrence of K chains in isolated cold haemagglutinins. Scand J Haematol. 1965;2(3):259–266. doi: 10.1111/j.1600-0609.1965.tb01303.x. [DOI] [PubMed] [Google Scholar]
  7. MARSH W. L., JENKINS W. J. Anti-i: a new cold antibody. Nature. 1960 Nov 26;188:753–753. doi: 10.1038/188753a0. [DOI] [PubMed] [Google Scholar]
  8. POLLACK W., HAGER H. J., RECKEL R., TOREN D. A., SINGHER H. A STUDY OF THE FORCES INVOLVED IN THE SECOND STAGE OF HEMAGGLUTINATION. Transfusion. 1965 Mar-Apr;5:158–183. doi: 10.1111/j.1537-2995.1965.tb01152.x. [DOI] [PubMed] [Google Scholar]
  9. Tönder O., Milgrom F. Studies on agglutination by macromolecular antibodies. I. Agglutination of rabbit erythrocytes by human sera. Vox Sang. 1965 Nov-Dec;10(6):708–720. doi: 10.1111/j.1423-0410.1965.tb05182.x. [DOI] [PubMed] [Google Scholar]
  10. WIENER A. S., UNGER L. J., COHEN L., FELDMAN J. Type-specific cold auto-antibodies as a cause of acquired hemolytic anemia and hemolytic transfusion reactions: biologic test with bovine red cells. Ann Intern Med. 1956 Feb;44(2):221–240. doi: 10.7326/0003-4819-44-2-221. [DOI] [PubMed] [Google Scholar]

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