Abstract
A technique is described for perfusing the isolated rat liver with viable bacteria and for comparing their phagocytosis by Kupffer cells when different suspending fluids are used successively.
Various general factors that modify uptake of bacteria have been studied, notably the nature of the bacteria and their concentration, the flow rate through the liver, the age of the liver preparation and the effect of repeated perfusion.
Normal human, rat and mouse sera were found to exert an opsonic effect on the phagocytosis of Escherichia coli. Thus the average uptake of these bacteria suspended in Ringer-Locke solution was 11 per cent, as compared with 41 per cent in serum. A reduction in opsonic activity of human serum was produced by heat at 56° for 30 min., absorption with the homologous strain or with an antigen-antibody system. Activity was completely abolished by homologous absorption followed by heat at 56° or absorption with an antigen-antibody system or with zymosan. Full opsonic activity was shown by a mixture of heated serum and E. coli-absorbed serum.
It is concluded that the serum factors that contribute to opsonic activity in this system are specific antibody, complement and another heat-labile factor. The possible identity of the latter with properdin is considered.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- PILLEMER L., BLUM L., LEPOW I. H., WURZ L., TODD E. W. The properdin system and immunity. III. The zymosan assay of properdin. J Exp Med. 1956 Jan 1;103(1):1–13. doi: 10.1084/jem.103.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROWLEY D., ALI W., JENKIN C. R. A reaction between fresh serum and lipopolysaccharides of gram-negative bacteria. Br J Exp Pathol. 1958 Feb;39(1):90–98. [PMC free article] [PubMed] [Google Scholar]
- RUTENBURG S. H., FINE J. Host resistance in hemorrhagic shock. VIII. Effect of properdin on in vitro function of phagocytes. Proc Soc Exp Biol Med. 1957 Jul;95(3):446–448. doi: 10.3181/00379727-95-23246. [DOI] [PubMed] [Google Scholar]
- WARDLAW A. C., PILLEMER L. The properdin system and immunity. V. The bactericidal activity of the properdin system. J Exp Med. 1956 May 1;103(5):553–575. doi: 10.1084/jem.103.5.553. [DOI] [PMC free article] [PubMed] [Google Scholar]