Abstract
Antisera have been prepared to adrenocorticotrophic hormone, thyrotrophic hormone and growth hormone extracted from human pituitaries and to gonadotrophin, and thyrotrophic hormone extracted from human urine.
Precipitin, absorption-precipitin and gel-diffusion tests have been performed with these antisera.
No conclusive evidence was obtained of the presence of a hormone-specific antibody in any of the antisera.
Cross-reactions occurred indicating the presence of an antigen common to those extracts prepared from the pituitary and a further antigen, or antigens, common to those extracts prepared from the urine.
Despite the absence of hormone-specific antibodies fluorescein-labelled samples of the antisera gave staining in the cytoplasm of cells in the anterior pituitary and did not stain other tissues.
The implication of these results in the interpretation of tissue localization studies with labelled antisera is discussed.
Full text
PDF













Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BARRNETT R. J., LADMAN A. J., MCALLASTER N. J., SIPERSTEIN E. R. The localization of glycoprotein hormones in the anterior pituitary glands of rats investigated by differential protein solubilities, histological stains and bio-assays. Endocrinology. 1956 Oct;59(4):398–418. doi: 10.1210/endo-59-4-398. [DOI] [PubMed] [Google Scholar]
- CLAYTON B. E., HARDISTY R. M., PINNIGER J. L. Studies on antibody formation to ACTH in man. J Endocrinol. 1952 Jan;8(1):56–63. doi: 10.1677/joe.0.0080056. [DOI] [PubMed] [Google Scholar]
- COONS A. H., LEDUC E. H., KAPLAN M. H. Localization of antigen in tissue cells. VI. The fate of injected foreign proteins in the mouse. J Exp Med. 1951 Feb;93(2):173–188. doi: 10.1084/jem.93.2.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CRUICKSHANK B., CURRIE A. R., DEKANSKI J. B., SKINNER L. G. Extraction of thyrotrophic hormone from human pituitaries. Nature. 1956 Nov 24;178(4543):1172–1173. doi: 10.1038/1781172a0. [DOI] [PubMed] [Google Scholar]
- CRUICKSHANK B., HILL A. G. The histochemical identification of a connective-tissue antigen in the rat. J Pathol Bacteriol. 1953 Jul;66(1):283–289. doi: 10.1002/path.1700660132. [DOI] [PubMed] [Google Scholar]
- CURRIE A. R., SYMINGTON T. The pathology of the pituitary and adrenal glands in systemic disease in man. Proc R Soc Med. 1955 Nov;48(11):908–909. [PMC free article] [PubMed] [Google Scholar]
- GOLDEN A., BONDY P. K., SHELDON W. H. Pituitary basophile hyperplasia and Crooke's hyaline changes in man after ACTH therapy. Proc Soc Exp Biol Med. 1950 Jun;74(2):455–458. doi: 10.3181/00379727-74-17938. [DOI] [PubMed] [Google Scholar]
- GOODMAN M., GREENSPON S. A., KRAKOWER C. A. The antigenic composition of the various anatomic structures of the canine kidney. J Immunol. 1955 Aug;75(2):96–104. [PubMed] [Google Scholar]
- GORDON G. L. The development of a refractory state to adrenocorticotrophic hormone. Endocrinology. 1949 Dec;45(6):571–580. doi: 10.1210/endo-45-6-571. [DOI] [PubMed] [Google Scholar]
- HALMI N. S. Two types of basophils in the anterior pituitary of the rat and their respective cytophysiological significance. Endocrinology. 1950 Oct;47(4):289–299. doi: 10.1210/endo-47-4-289. [DOI] [PubMed] [Google Scholar]
- HEWETT C. L., CRUICKSHANK B., CURRIE A. R. Extraction of ACTH from human pituitaries. Nature. 1954 Oct 16;174(4433):745–746. doi: 10.1038/174745a0. [DOI] [PubMed] [Google Scholar]
- HUMPHREY J. H. Origin of blood platelets. Nature. 1955 Jul 2;176(4470):38–38. doi: 10.1038/176038a0. [DOI] [PubMed] [Google Scholar]
- LAQUEUR G. L. Cytological changes in human hypophyses after cortisone and ACTH treatment. Science. 1950 Oct 13;112(2911):429–430. doi: 10.1126/science.112.2911.429-a. [DOI] [PubMed] [Google Scholar]
- MELLORS R. C., ARIAS-STELLA J., SIEGEL M., PRESSMAN D. Analytical pathology. II. Histopathologic demonstration of glomerular-localizing antibodies in experimental glomerulonephritis. Am J Pathol. 1955 Jul-Aug;31(4):687–715. [PMC free article] [PubMed] [Google Scholar]
- MELLORS R. C., SIEGEL M., PRESSMAN D. Analytic pathology: histochemical demonstration of antibody localization in tissues, with special reference to the antigenic components of kidney and lung. Lab Invest. 1955 Mar-Apr;4(2):69–89. [PubMed] [Google Scholar]
- MORRISON J. F., BAZELEY P. L., ENNOR A. H., THAYER J. H. Serological studies on the antigenicity and purity of adrenocorticotrophic hormone and growth hormone. Br J Exp Pathol. 1952 Feb;33(1):48–56. [PMC free article] [PubMed] [Google Scholar]
- ORTEGA L. G., MELLORS R. C. Analytical pathology. IV. The role of localized antibodies in the pathogenesis of nephrotoxic nephritis in the rat. J Exp Med. 1956 Jul 1;104(1):151–157. doi: 10.1084/jem.104.1.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PERKOFF G. T., JAGER B. V., TYLER F. H. Complications in the management of Cushing's syndrome, including anaphylactic reaction to intravenous adrenocorticotropin after subtotal adrenalectomy. J Clin Endocrinol Metab. 1955 Mar;15(3):362–372. doi: 10.1210/jcem-15-3-362. [DOI] [PubMed] [Google Scholar]
- RUSSFIELD A. B. Histology of the human hypophysis in thyroid disease-hypothyroidism, hyperthyroidism, and cancer. J Clin Endocrinol Metab. 1955 Nov;15(11):1393–1408. doi: 10.1210/jcem-15-11-1393. [DOI] [PubMed] [Google Scholar]
- Rowlands I. W., Young F. G. The capacity of pituitary preparations containing the thyrotrophic hormone to induce the formation of antisera. J Physiol. 1939 Apr 14;95(3):410–419. doi: 10.1113/jphysiol.1939.sp003738. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SAFFRAN M., SCHALLY A. V. In vitro bioassay of corticotropin: modification and statistical treatment. Endocrinology. 1955 May;56(5):523–532. doi: 10.1210/endo-56-5-523. [DOI] [PubMed] [Google Scholar]
- SCOTT D. G. A study of the antigenicity of basement membrane and reticulin. Br J Exp Pathol. 1957 Apr;38(2):178–185. [PMC free article] [PubMed] [Google Scholar]
- VAN DYKE H. B., P'AN S. Y., SHEDLOVSKY T. Follicle-stimulating hormones of the anterior pituitary of the sheep and the hog. Endocrinology. 1950 Jun;46(6):563–573. doi: 10.1210/endo-46-6-563. [DOI] [PubMed] [Google Scholar]
- WEST H. F. Acquired resistance to corticotropins. Ann Rheum Dis. 1956 Jun;15(2):124–133. doi: 10.1136/ard.15.2.124. [DOI] [PMC free article] [PubMed] [Google Scholar]