Abstract
The agglutinin titres of tetanus antitoxins tested with preserved sheep cells sensitized with tetanus toxoid have been found to be inversely proportional to the cell concentration used.
The agglutinin titres per international unit of antitoxin were widely different among various electrophoretically separated globulin fractions of tetanus antitoxin. There were smaller differences between the agglutinating capacity of γ-globulin fractions of sera from different horses.
With haemagglutination inhibition tests there was always a relative increase in test dose between the LA and LA/100 level of test. This relative increase was greatest where the sensitivity of the cell suspensions was high.
Increased suspension sensitivity may be the result of treatment of cells with greater quantities of sensitizing antigen or of reduction in the concentration of sensitized cells in the suspension.
A greater volume of serum was required to give a standard end point, when mixed with a test dose (LA) of toxin, if the mixtures were allowed to stand for increasing periods of time. These observations have been discussed.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- FULTHORPE A. J. Tetanus antitoxin titration by haemagglutination. J Hyg (Lond) 1957 Sep;55(3):382–401. doi: 10.1017/s0022172400037293. [DOI] [PMC free article] [PubMed] [Google Scholar]