Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1959 Jul;2(3):262–267.

Blood Group Antigens on HeLa Cells shown by Mixed Agglutination

A Kelus, B W Gurner, R R A Coombs
PMCID: PMC1423951  PMID: 14405338

Abstract

The mixed agglutination reaction has been used for investigating the presence of blood group antigens on the surface of human cervical carcinoma cells (HeLa) cultured for eight years in vitro.

The H antigen was demonstrated in the absence of A and B. The MN-type antigen has been found as well as Tja.

Treatment of HeLa cells with ficin greatly enhanced the reaction of anti-H and anti-Tja with the corresponding antigens on HeLa cells. The authors failed to show the following antigens: Rh(D) and Rh(c), S, P, Lea, Leb, Lua and Lub.

Full text

PDF

Images in this article

265FIG. 1
on p.265
265FIG. 2
on p.265

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CAZAL P., LALAURIE M. Recherches sur quelques phyto-agglutinines spécifiques des groupes sanguins ABO. Acta Haematol. 1952 Jul-Aug;8(1-2):73–80. doi: 10.1159/000204150. [DOI] [PubMed] [Google Scholar]
  2. DAUSSET J., COLOMBANI J., EVELIN J. Présence de l'antigène Rh (D) dans les leucocytes et les plaquettes humaines. Vox Sang. 1958 Jul;3(4):266–276. doi: 10.1111/j.1423-0410.1958.tb03620.x. [DOI] [PubMed] [Google Scholar]
  3. GURNER B. W., COOMBS R. R. Examination of human leucocytes for the ABO, MN, Rh, Tja, Lutheran and Lewis systems of antigens by means of mixed erythrocyte-leucocyte agglutination. Vox Sang. 1958 Jan;3(1):13–22. doi: 10.1111/j.1423-0410.1958.tb03554.x. [DOI] [PubMed] [Google Scholar]
  4. KAY H. E. A and B antigens of normal and malignant cells. Br J Cancer. 1957 Sep;11(3):409–414. doi: 10.1038/bjc.1957.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. MOULINIER J., SERVANTIE X. Détection par le test de consommation d'antiglobuline de l'antigène D sur les plaquettes des individus Rh-positif (D+). Vox Sang. 1958 Jul;3(4):277–283. doi: 10.1111/j.1423-0410.1958.tb03621.x. [DOI] [PubMed] [Google Scholar]
  6. NEWTON A., STOKER M. G. Changes in nucleic acid content of HeLa cells infected with herpes virus. Virology. 1958 Jun;5(3):549–560. doi: 10.1016/0042-6822(58)90044-8. [DOI] [PubMed] [Google Scholar]
  7. SCHERER W. F., SYVERTON J. T., GEY G. O. Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix. J Exp Med. 1953 May;97(5):695–710. doi: 10.1084/jem.97.5.695. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES