Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1960 Apr;3(2):179–189.

in vitro

Production of Pyrogenic Substances in the Reaction of Cells of Hyper-sensitive Guinea Pigs with Antigen

J Johanovský
PMCID: PMC1423979  PMID: 14407368

Abstract

Previous findings of the appearance of pyrogenic activity when spleen and lymph node cells from BCG-sensitized rabbits were incubated with tuberculin in vitro have been repeated and extended in experiments with guinea pigs. Two different systems were used: hypersensitivity induced by BCG infection and by means of diphtheria toxoid-antitoxin precipitates. Cells were incubated in vitro with diluted tuberculin, diphtheria toxoid or Hanks's solution. Supernatant fluids obtained after various incubation periods were tested in normal guinea pigs for their pyrogenic activity and for ability to induce skin inflammatory reactions.

Pyrogenic substances were obtained in most of the experiments in which cells of hypersensitive animals were incubated with the specific antigen; experiments with the unrelated antigen and controls were negative. Formation of skin-irritating substances was less regular, and they were sometimes formed non-specifically in control experiments also; in general, however, the results of these experiments agreed with the results of the fever reaction.

The results obtained are discussed in relation to the mechanism of delayed hypersensitivity.

Full text

PDF
179

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERDEL W., BUDDECKE E. Die allergische Stufenreaktion im tuberkulösen Organismus. Beitr Klin Tuberk Spezif Tuberkuloseforsch. 1950;104(3):214–240. [PubMed] [Google Scholar]
  2. CARRERE L., QUATREFAGES H. D'un phénomène observé au cours du transfert local de l'allergie à la mélitine. C R Hebd Seances Acad Sci. 1952 Jan 21;234(4):483–484. [PubMed] [Google Scholar]
  3. HOWARD J. G., BIOZZI G., HALPERN B. N., STIFFEL C., MOUTON D. The effect of Mycobacterium tuberculosis (BCG) infection on the resistance of mice to bacterial endotoxin and Salmonella enteritidis infection. Br J Exp Pathol. 1959 Jun;40(3):281–290. [PMC free article] [PubMed] [Google Scholar]
  4. INDERBITZIN T. The relationship of lymphocytes, delayed cutaneous allergic reactions and histamine. Int Arch Allergy Appl Immunol. 1956;8(3):150–159. doi: 10.1159/000228277. [DOI] [PubMed] [Google Scholar]
  5. PANGBORN M. C., BIRKHAUG C. A purified tuberculin fraction from unheated cultures in testing BCG-vaccinated subjects. Am Rev Tuberc. 1954 Feb;69(2):300–303. doi: 10.1164/art.1954.69.2.300. [DOI] [PubMed] [Google Scholar]
  6. PAPPENHEIMER A. M., SALVIN S. B., UHR J. W. Delayed hypersensitivity. II. Induction of hypersensitivity in guinea pigs by means of antigen-antibody complexes. J Exp Med. 1957 Jan 1;105(1):11–24. doi: 10.1084/jem.105.1.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. RAFFEL S., NEWEL J. M. The delayed hypersensitivity induced by antigen-antibody complexes. J Exp Med. 1958 Dec 1;108(6):823–841. doi: 10.1084/jem.108.6.823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. UHR J. W., BRANDRISS M. W. Delayed hypersensitivity. IV. Systemic reactivity of guinea pigs sensitized to protein antigens. J Exp Med. 1958 Dec 1;108(6):905–924. doi: 10.1084/jem.108.6.905. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. WAKSMAN B. H., MATOLTSY M. Quantitative study of local passive transfer of tuberculin sensitivity with peritoneal exudate cells in the guinea pig. J Immunol. 1958 Sep;81(3):235–241. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES