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. 1962 Nov;5(6):673–686.

Study of the Antigenic Constituents of Sera from Mouse/Rat Chimaeras

P Grabar, J Courcon, D W H Barnes, C E Ford, H S Micklem
PMCID: PMC1424085  PMID: 13949661

Abstract

The sera of CBA mice lethally irradiated and restored with rat bone marrow or foetal liver have been examined by immunoelectrophoresis at various times after irradiation. Such sera contain several specific rat proteins including γ globulin, at least two α2 globulins, one or two β1 globulins and one β2 globulin. A large number of specific mouse proteins are also present, but no mouse γ globulins have been detected.

The rat constituents appear progressively and persist for long periods; this implies that they are synthesized by rat cells.

There is a close correlation between the presence of rat γ globulin in the serum and the presence of dividing rat cells in the bone marrow and spleen.

The fact that γ globulin is solely of rat specificity does not necessarily rule out the possibility of immune activity by the host against the graft.

Several of the normal serum proteins, in addition to the γ globulins, originate from the injected cells, and are therefore presumably not synthesized by the liver.

In chimaeras grafted with rat skin, the number of specific rat serum proteins is often larger than the number in chimaeras which have not been grafted.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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