Abstract
Rabbits previously immunized to two antigens were restimulated by the injection of one antigen followed, 1 day later, by the second. Spleens were removed 2 days after the injection of the second antigen and a cell suspension prepared. Such preparations synthesized antibody to both antigens stimultaneously when incubated in vitro. The system-synthesizing antibody to the first antigen, however, was 1 day in advance of the other, developmentally.
The effect of various metabolic inhibitors on cell survival and the incorporation of radioactive amino acid into each antibody and into cell protein was measured. By comparing the effect of each inhibitor on these four parameters it was found that the inhibition of antibody synthesis by ethidium bromide, mitomycin C, 6-mercaptopurine, 5,6-dichlorobenzimidazole riboside, 6-thioguanosine and X-irradiation was secondary to a general derangement of cell metabolism. In concontrast, the thymidine analogues, 6-azauridine and possibly colchicine exerted a more selective effect on the antibody formation mechanism. The thymidine analogues and 6-azauridine did not appear to inhibit antibody synthesis per se, but to prevent the development of antibody-forming capacity. The significance of these results is discussed.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ALLFREY V. G., MIRSKY A. E., OSAWA S. Protein synthesis in isolated cell nuclei. J Gen Physiol. 1957 Jan 20;40(3):451–490. doi: 10.1085/jgp.40.3.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BERENBAUM M. C. Effect of cytotoxic agents on antibody production. Nature. 1960 Jan 16;185:167–168. doi: 10.1038/185167a0. [DOI] [PubMed] [Google Scholar]
- DUTTON R. W., DUTTON A. H., VAUGHAN J. H. The effect of 5-bromouracil deoxyriboside on the synthesis of antibody in vitro. Biochem J. 1960 May;75:230–235. doi: 10.1042/bj0750230. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MANDEL H. G. The physiological disposition of some anticancer agents. Pharmacol Rev. 1959 Dec;11:743–838. [PubMed] [Google Scholar]
- REICH E., SHATKIN A. J., TATUM E. L. Bacteriocidal action of mitomycin C. Biochim Biophys Acta. 1960 Dec 18;45:608–610. doi: 10.1016/0006-3002(60)91504-3. [DOI] [PubMed] [Google Scholar]
- SHIBA S., TERAWAKI A., TAGUCHI T., KAWAMATA J. Selective inhibition of formation of deoxyribonucleic acid in Escherichia coli by mitomycin C. Nature. 1959 Apr 11;183(4667):1056–1057. doi: 10.1038/1831056a0. [DOI] [PubMed] [Google Scholar]
- VAUGHAN J. H., DUTTON A. H., DUTTON R. W., GEORGE M., MARSTON R. Q. A study of antibody production in vitro. J Immunol. 1960 Mar;84:258–267. [PubMed] [Google Scholar]