Abstract
Specific precipitates formed in 0.9 per cent and 8 per cent NaCl and the precipitates formed by raising the salt concentration of `0.9 per cent' supernatants to 8 per cent were measured quantitatively. With antisera to haemoglobin and myoglobin the antigen in the precipitates was also measured. Except for some very high antibody/antigen ratios found in some cases in antibody excess, these ratios were the same as those found with rabbit antibody, and did not depend on salt concentration.
Non-precipitating antibody, prepared by serial absorption of antiserum with small portions of antigen, did not precipitate with antigen even in 8 per cent NaCl; it co-precipitated with homologous rabbit antiserum and delayed its flocculation, but produced no permanent inhibition.
Rabbit antiserum to washed specific precipitates made from fowl antisera was used to confirm the presence of two globulins, one a macroglobulin, in the precipitates, and to study their different properties when free in whole serum and when combined with antigen.
Full text
PDFImages in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BANOVITZ J., WOLFE H. R. Precipitin production in chickens. XIX. The components of chicken antiserum involved in the precipitin reaction. J Immunol. 1959 Jun;82(6):489–496. [PubMed] [Google Scholar]
- DEUTSCH H. F., NICHOL J. C., COHN M. Biophysical studies of blood plasma proteins; immunological and electrophoretic studies of immune chicken serum. J Immunol. 1949 Oct;63(2):195–210. [PubMed] [Google Scholar]
- GENGOZIAN N., WOLFE H. R. Precipitin production in chickens. XV. The effect of aging of the antisera on precipitate formation. J Immunol. 1957 Jun;78(6):401–408. [PubMed] [Google Scholar]
- GOODMAN M., WOLFE H. R., NORTON S. Precipitin production in chickens. VI. The effect of varying concentrations of NaCl on precipitate formation. J Immunol. 1951 Feb;66(2):225–236. [PubMed] [Google Scholar]
- MAKINODAN T., GENGOZIAN N., CANNING R. E. Demonstration of a normal serum macroglobulin coprecipitating with the bovine serum albumin (BSA)-chicken anti-BSA precipitate. J Immunol. 1960 Nov;85:439–446. [PubMed] [Google Scholar]
- ORLANS E., ROSE M. E., MARRACK J. R. Fowl antibody. I. Some physical and immunochemical properties. Immunology. 1961 Jul;4:262–277. [PMC free article] [PubMed] [Google Scholar]
- OWEN J. A., SILBERMAN H. J., GOT C. Detection of haemoglobin, haemoglobin-haptoglobin complexes and other substances with peroxidase activity after zone electrophoresis. Nature. 1958 Nov 15;182(4646):1373–1373. doi: 10.1038/1821373a0. [DOI] [PubMed] [Google Scholar]
- WEIGLE W. O., MAURER P. H. The effect of complements on soluble antigen-antibody complexes. J Immunol. 1957 Sep;79(3):211–222. [PubMed] [Google Scholar]