Abstract
The injection of normal guinea-pig serum in Freund's complete adjuvant into guinea pigs other than the serum donor led to the development of a long-lasting, delayed hypersensitivity. Serum alone, without adjuvant, had no sensitizing capacity. Circulating antibodies to the allotypic antigens could not be detected.
Injection of as much as 8 ml. of serum into sensitized animals did not achieve desensitization. However, the intravenous injection of the same amount of serum prevented normal guinea pigs from becoming sensitized to the same antigen for over 100 days. This unresponsiveness was interpreted to be due to an interference by the serum with the process of sensitization.
This method of producing unresponsiveness was applied to the homograft reaction: guinea pigs, given a series of intravenous injections of spleen extracts containing transplantation antigens, could not be subsequently sensitized by the injection of spleen cells from the same donors. However, immunization provided by skin grafting could probably break through this unresponsiveness: guinea pigs, judged to be unresponsive by the intradermal injection of spleen cells before skin grafting, all developed an intense cutaneous hypersensitivity after they had rejected the graft.
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Selected References
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