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. 2003 Feb;185(4):1484. doi: 10.1128/JB.185.4.1484.2003

Construction, Characterization, and Use of Two Listeria monocytogenes Site-Specific Phage Integration Vectors

Peter Lauer 1, Man Yin Nora Chow 1, Martin J Loessner 1, Daniel A Portnoy 1, Richard Calendar 1
PMCID: PMC142897

Volume 184, no. 15, p. 4177-4186, 2002. Page 4178, Fig. 1B: the HindIII site, at base pair position 69, indicated as unique in the multiple cloning site (MCS) is not unique. There are two additional HindIII sites in the PSA integrase gene at base pair positions 3244 and 3454 of the pPL2 sequence (GenBank accession no. AJ417449). Hence, there are only 12 unique restriction sites in the pPL2 MCS.


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