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. 1979 Apr;7(4):333–341. doi: 10.1111/j.1365-2125.1979.tb00942.x

The effect of assay methods on plasma levels and pharmacokinetics of theophylline: HPLC and EIA.

T Ishizaki, M Watanabe, N Morishita
PMCID: PMC1429655  PMID: 375957

Abstract

1 The effects of two assay methods, HPLC and EIA, on theophylline plasma levels (n = 254) and pharmacokinetics (n = 6) were examined on blood samples obtained from asthmatic patients and normal adult male volunteers. 2 The standard curves obtained and the plasma level values during plasma level monitoring of theophylline measured with two methods are well correlated each other within concentration range of 2.5 to 20.0 microgram/ml (P less than 0.001). EIA, however in general, appears to give a lower value than HPLC. 3 The values of plasma theophylline half-life and clearance analyzed using a two-compartment open system model were found different between the two assay methods and dissimilar to those previously reported by using ultraviolet method. 4 The sampling end-point and/or assay sensitivity particularly to measure a lower level of theophylline (less than 2.5 microgram/ml) can influence the pharmacokinetics for a potent bronchodilator with a narrow therapeutic margin, theophylline.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams R. F., Vandemark F. L., Schmidt G. J. More sensitive high-pressure liquid-chromatographic determiantion of theophylline in serum. Clin Chem. 1976 Nov;22(11):1903–1906. [PubMed] [Google Scholar]
  2. Chrzanowski F. A., Niebergall P. J., Mayock R. L., Taubin J. M., Sugita E. T. Kinetics of intravenous theophylline. Clin Pharmacol Ther. 1977 Aug;22(2):188–195. doi: 10.1002/cpt1977222188. [DOI] [PubMed] [Google Scholar]
  3. Ellis E. F., Koysooko R., Levy G. Pharmacokinetics of theophylline in children with asthma. Pediatrics. 1976 Oct;58(4):542–547. [PubMed] [Google Scholar]
  4. Floyd R. A., Cohen J. L. Pharmacokinetics in drug therapy II: Applications of clinical pharmacokinetics in the assessment of theophylline therapy. Am J Hosp Pharm. 1977 Apr;34(4):402–407. [PubMed] [Google Scholar]
  5. Gibaldi M., Weintraub H. Some considerations as to the determination and significance of biologic half-life. J Pharm Sci. 1971 Apr;60(4):624–626. doi: 10.1002/jps.2600600430. [DOI] [PubMed] [Google Scholar]
  6. Hendeles L., Weinberger M., Bighley L. Disposition of theophylline after a single intravenous infusion of aminophylline. Am Rev Respir Dis. 1978 Jul;118(1):97–103. doi: 10.1164/arrd.1978.118.1.97. [DOI] [PubMed] [Google Scholar]
  7. Hendeles L., Weinberger M., Johnson G. Monitoring serum theophylline levels. Clin Pharmacokinet. 1978 Jul-Aug;3(4):294–312. doi: 10.2165/00003088-197803040-00003. [DOI] [PubMed] [Google Scholar]
  8. Hendeles L., Weinberger M. Kinetics of intravenous theophylline. Clin Pharmacol Ther. 1977 Dec;22(6):936–936. doi: 10.1002/cpt1977226936. [DOI] [PubMed] [Google Scholar]
  9. Jacobs M. H., Senior R. M., Kessler G. Clinical experience with theophylline. Relarionships between dosage, serum concentration, and toxicity. JAMA. 1976 May 3;235(18):1983–1986. doi: 10.1001/jama.235.18.1983. [DOI] [PubMed] [Google Scholar]
  10. Jenne J. W., Wyze M. S., Rood F. S., MacDonald F. M. Pharmacokinetics of theophylline. Application to adjustment of the clinical dose of aminophylline. Clin Pharmacol Ther. 1972 May-Jun;13(3):349–360. doi: 10.1002/cpt1972133349. [DOI] [PubMed] [Google Scholar]
  11. Kappas A., Anderson K. E., Conney A. H., Alvares A. P. Influence of dietary protein and carbohydrate on antipyrine and theophylline metabolism in man. Clin Pharmacol Ther. 1976 Dec;20(6):643–653. doi: 10.1002/cpt1976206643. [DOI] [PubMed] [Google Scholar]
  12. Kordash T. R., Van Dellen R. G., McCall J. T. Theophylline concentrations in asthmatic patients after administration of aminophylline. JAMA. 1977 Jul 11;238(2):139–141. [PubMed] [Google Scholar]
  13. Koup J. R., Schentag J. J., Vance J. W., Kuritzky P. M., Pyszczynski D. R., Jusko W. J. System for clinical pharmacokinetic monitoring of theophylline therapy. Am J Hosp Pharm. 1976 Sep;33(9):949–956. [PubMed] [Google Scholar]
  14. Leung P., Kalisker A., Bell T. D. Variation in theophylline clearance rate with time in chronic childhood asthma. J Allergy Clin Immunol. 1977 Jun;59(6):440–444. doi: 10.1016/0091-6749(77)90007-0. [DOI] [PubMed] [Google Scholar]
  15. Levy G., Koysooko R. Pharmacokinetic analysis of the effect of theophylline on pulmonary function in asthmatic children. J Pediatr. 1975 May;86(5):789–793. doi: 10.1016/s0022-3476(75)80374-x. [DOI] [PubMed] [Google Scholar]
  16. Loughnan P. M., Sitar D. S., Ogilvie R. I., Neims A. H. The two-compartment open-system kinetic model: a review of its clinical implications and applications. J Pediatr. 1976 May;88(5):869–873. doi: 10.1016/s0022-3476(76)81135-3. [DOI] [PubMed] [Google Scholar]
  17. Mitenko P. A., Ogilvie R. I. Pharmacokinetics of intravenous theophylline. Clin Pharmacol Ther. 1973 Jul-Aug;14(4):509–513. doi: 10.1002/cpt1973144part1509. [DOI] [PubMed] [Google Scholar]
  18. Mitenko P. A., Ogilvie R. I. Rapidly achieved plasma concentration plateaus, with observations on theophylline kinetics. Clin Pharmacol Ther. 1972 May-Jun;13(3):329–335. doi: 10.1002/cpt1972133329. [DOI] [PubMed] [Google Scholar]
  19. Mitenko P. A., Ogilvie R. I. Rational intravenous doses of theophylline. N Engl J Med. 1973 Sep 20;289(12):600–603. doi: 10.1056/NEJM197309202891202. [DOI] [PubMed] [Google Scholar]
  20. Nayak R. K., Smyth R. D., Chamberlain J. H., Polk A., DeLong A. F., Herczeg T., Chemburkar P. B., Joslin R. S., Reavey-Cantwell N. H. Methaqualone pharmacokinetics after single- and multiple-dose administration in man. J Pharmacokinet Biopharm. 1974 Apr;2(2):107–121. doi: 10.1007/BF01061503. [DOI] [PubMed] [Google Scholar]
  21. Ogilvie R. I. Clinical pharmacokinetics of theophylline. Clin Pharmacokinet. 1978 Jul-Aug;3(4):267–293. doi: 10.2165/00003088-197803040-00002. [DOI] [PubMed] [Google Scholar]
  22. Powell J. R., Thiercelin J. F., Vozeh S., Sansom L., Riegelman S. The influence of cigarette smoking and sex on theophylline disposition. Am Rev Respir Dis. 1977 Jul;116(1):17–23. doi: 10.1164/arrd.1977.116.1.17. [DOI] [PubMed] [Google Scholar]
  23. Powell J. R., Vozeh S., Hopewell P., Costello J., Sheiner L. B., Riegelman S. Theophylline disposition in acutely ill hospitalized patients. The effect of smoking, heart failure, severe airway obstruction, and pneumonia. Am Rev Respir Dis. 1978 Aug;118(2):229–238. doi: 10.1164/arrd.1978.118.2.229. [DOI] [PubMed] [Google Scholar]
  24. SCHACK J. A., WAXLER S. H. An ultraviolet spectrophotometric method for the determination of theophylline and theobromine in blood and tissues. J Pharmacol Exp Ther. 1949 Nov;97(3):283–291. [PubMed] [Google Scholar]
  25. Scharpé S. L., Cooreman W. M., Blomme W. J., Laekeman G. M. Quantitative enzyme immunoassay: current status. Clin Chem. 1976 Jun;22(6):733–738. [PubMed] [Google Scholar]
  26. Schentag J. J., Jusko W. J., Plaut M. E., Cumbo T. J., Vance J. W., Abrutyn E. Tissue persistence of gentamicin in man. JAMA. 1977 Jul 25;238(4):327–329. [PubMed] [Google Scholar]
  27. Walson P. D., Strunk R. C., Taussig L. M. Intrapatient variability in theophylline kinetics. J Pediatr. 1977 Aug;91(2):321–324. doi: 10.1016/s0022-3476(77)80844-5. [DOI] [PubMed] [Google Scholar]
  28. Weinberger M. W., Matthay R. A., Ginchansky E. J., Chidsey C. A., Petty T. L. Intravenous aminophylline dosage. Use of serum theophylline measurement for guidance. JAMA. 1976 May 10;235(19):2110–2113. doi: 10.1001/jama.235.19.2110. [DOI] [PubMed] [Google Scholar]
  29. Weinberger M., Riegelman S. Editorial: Rational use of theophylline for bronchodilatation. N Engl J Med. 1974 Jul 18;291(3):151–153. doi: 10.1056/NEJM197407182910310. [DOI] [PubMed] [Google Scholar]
  30. Wisdom G. B. Enzyme-immunoassay. Clin Chem. 1976 Aug;22(8):1243–1255. [PubMed] [Google Scholar]
  31. Zwillich C. W., Sutton F. D., Neff T. A., Cohn W. M., Matthay R. A., Weinberger M. M. Theophylline-induced seizures in adults. Correlation with serum concentrations. Ann Intern Med. 1975 Jun;82(6):784–787. doi: 10.7326/0003-4819-82-6-784. [DOI] [PubMed] [Google Scholar]

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