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. 1984 Jun;25(6):636–643. doi: 10.1136/gut.25.6.636

Gastrointestinal regulatory peptide storage granule abnormalities in jejunal mucosal diseases.

J Dawson, M G Bryant, S R Bloom, T J Peters
PMCID: PMC1432371  PMID: 6145662

Abstract

The mucosal concentrations of seven regulatory peptides and the density properties and integrity of their storage granules have been studied in mucosal biopsies from the human jejunum in eight gastrointestinal disease states and compared with normal controls. In diseases with associated mucosal inflammation (coeliac disease, Crohn's disease with jejunal involvement, postinfective tropical malabsorption, and common variable immunodeficiency) there was a selective increase in fragility of the gastric inhibitory polypeptide (GIP) and somatostatin storage granules. The gastrin, motilin, enteroglucagon, secretin, and vasoactive intestinal polypeptide granules had normal properties in these conditions. In diseases in which diarrhoea occurred in the absence of changes in jejunal mucosal histology (irritable bowel syndrome, pancreatic insufficiency, jejuno-ileal bypass for morbid obesity, and purgative abuse) there were no abnormalities of the storage granules. Increased mucosal concentrations of all peptides except vasoactive intestinal polypeptide (VIP) were found in coeliac disease and selective increases of VIP found in Crohn's disease, motilin in the irritable bowel syndrome and gastrin and GIP in pancreatic insufficiency. It is suggested that the storage granule abnormalities in the diseases with abnormal mucosal histology are secondary to the inflammatory changes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adrian T. E., Barnes A. J., Long R. G., O'Shaughnessy D. J., Brown M. R., Rivier J., Vale W., Blackburn A. M., Bloom S. R. The effect of somatostatin analogs on secretion of growth, pancreatic, and gastrointestinal hormones in man. J Clin Endocrinol Metab. 1981 Oct;53(4):675–681. doi: 10.1210/jcem-53-4-675. [DOI] [PubMed] [Google Scholar]
  2. Besterman H. S., Bloom S. R., Sarson D. L., Blackburn A. M., Johnston D. I., Patel H. R., Stewart J. S., Modigliani R., Guerin S., Mallinson C. N. Gut-hormone profile in coeliac disease. Lancet. 1978 Apr 15;1(8068):785–788. doi: 10.1016/s0140-6736(78)92994-x. [DOI] [PubMed] [Google Scholar]
  3. Besterman H. S., Cook G. C., Sarson D. L., Christofides N. D., Bryant M. G., Gregor M., Bloom S. R. Gut hormones in tropical malabsorption. Br Med J. 1979 Nov 17;2(6200):1252–1255. doi: 10.1136/bmj.2.6200.1252. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Besterman H. S., Sarson D. L., Rambaud J. C., Stewart J. S., Guerin S., Bloom S. R. Gut hormone responses in the irritable bowel syndrome. Digestion. 1981;21(4):219–224. doi: 10.1159/000198566. [DOI] [PubMed] [Google Scholar]
  5. Bishop A. E., Polak J. M., Bryant M. G., Bloom S. R., Hamilton S. Abnormalities of vasoactive intestinal polypeptide-containing nerves in Crohn's disease. Gastroenterology. 1980 Nov;79(5 Pt 1):853–860. [PubMed] [Google Scholar]
  6. Bloom S. R., Polak J. M. Plasma hormone concentrations in gastrointestinal disease. Clin Gastroenterol. 1980 Sep;9(3):785–798. [PubMed] [Google Scholar]
  7. Bryant M. G., Bloom S. R. Distribution of the gut hormones in the primate intestinal tract. Gut. 1979 Aug;20(8):653–659. doi: 10.1136/gut.20.8.653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Bryant M. G., Dawson J., Bloom S. R., Peters T. J. Separation of the gut hormone endocrine-cell storage granules of human jejunum using analytical subcellular fractionation. Gut. 1980 Mar;21(3):177–180. doi: 10.1136/gut.21.3.177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. DAVIS D. R., DOCKERTY M. B., MAYO C. W. The myenteric plexus in regional enteritis: a study of the number of ganglion cells in the ileum in 24 cases. Surg Gynecol Obstet. 1955 Aug;101(2):208–216. [PubMed] [Google Scholar]
  10. Dawson J., Bryant M. G., Bloom S. R., Peters T. J. Subcellular fractionation studies of human gastric antrum: localization of the mucosal peptide hormones. Clin Sci (Lond) 1980 Jul;59(1):1–6. doi: 10.1042/cs0590001. [DOI] [PubMed] [Google Scholar]
  11. Geoghegan J., Fielding J. F. Serum gastrin and the irritable bowel syndrome. Ir J Med Sci. 1978 Apr;147(4):156–156. doi: 10.1007/BF02939391. [DOI] [PubMed] [Google Scholar]
  12. HIRAOKA T., GLICK D. Studies in histochemistry. LXXI. Measurement of protein in millimicrogram amounts by quenching of dye fluorescence. Anal Biochem. 1963 Jun;5:497–504. doi: 10.1016/0003-2697(63)90069-1. [DOI] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROBERTS N. R., KAPPHAHN J. I. The fluorometric measurement of pyridine nucleotides. J Biol Chem. 1957 Feb;224(2):1047–1064. [PubMed] [Google Scholar]
  14. O'Connor F. A., McLoughlin J. C., Buchanan K. D. Impaired immunoreactive secretin release in coeliac disease. Br Med J. 1977 Mar 26;1(6064):811–811. doi: 10.1136/bmj.1.6064.811. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Peters T. J. Analytical subcellular fractionation of jejunal biopsy specimens: methodology and characterization of the organelles in normal tissue. Clin Sci Mol Med. 1976 Dec;51(6):557–574. doi: 10.1042/cs0510557. [DOI] [PubMed] [Google Scholar]
  16. Peters T. J., Heath J. R., Wansbrough-Jones M. H., Foe W. F. Enzyme activities and properties of lysosomes and brush borders in jejunal biopsies from control subjects and patients with coeliac disease. Clin Sci Mol Med. 1975 Apr;48(4):259–267. doi: 10.1042/cs0480259. [DOI] [PubMed] [Google Scholar]
  17. Peters T. J., Jones P. E., Wells G., Cook G. G. Sequential enzyme and subcellular fractionation studies on jejunal biopsy specimens from patients with post-infective tropical malabsorption. Clin Sci (Lond) 1979 May;56(5):479–486. doi: 10.1042/cs0560479. [DOI] [PubMed] [Google Scholar]
  18. Polak J. M., Pearse A. G., Van Noorden S., Bloom S. R., Rossiter M. A. Secretin cells in coeliac disease. Gut. 1973 Nov;14(11):870–874. doi: 10.1136/gut.14.11.870. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rhodes R. A., Tai H. H., Chey W. Y. Impairment of secretin release in celiac sprue. Am J Dig Dis. 1978 Sep;23(9):833–839. doi: 10.1007/BF01079794. [DOI] [PubMed] [Google Scholar]
  20. Sjölund K., Alumets J., Berg N. O., Håkanson R., Sundler F. Duodenal endocrine cells in adult coeliac disease. Gut. 1979 Jul;20(7):547–552. doi: 10.1136/gut.20.7.547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Vantrappen G., Janssens J., Peeters T. L., Bloom S. R., Christofides N. D., Hellemans J. Motilin and the interdigestive migrating motor complex in man. Dig Dis Sci. 1979 Jul;24(7):497–500. doi: 10.1007/BF01489315. [DOI] [PubMed] [Google Scholar]

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