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. 1984 Sep;25(9):925–930. doi: 10.1136/gut.25.9.925

Early organogenesis of human small intestine: scanning electron microscopy and brush border enzymology.

B Lacroix, M Kedinger, P Simon-Assmann, K Haffen
PMCID: PMC1432492  PMID: 6432634

Abstract

Human small bowel early organogenesis was studied by scanning electron microscopy and found to be correlated to brush border enzymology. The appearance of the brush border enzymes sucrase, lactase, and aminopeptidase (measured in a purified apical membrane fraction) coincides with the first outgrowth of villi (eight weeks). Alkaline phosphatase was detected at seven weeks. The content of these enzymes furthermore increased up to the 14th week when both sucrase and aminopeptidase activities were comparable with adult values.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Antonowicz I., Chang S. K., Grand R. J. Development and distribution of lysosomal enzymes and disaccharidases in human fetal intestine. Gastroenterology. 1974 Jul;67(1):51–58. [PubMed] [Google Scholar]
  2. Antonowicz I., Lebenthal E. Developmental pattern of small intestinal enterokinase and disaccharidase activities in the human fetus. Gastroenterology. 1977 Jun;72(6):1299–1303. [PubMed] [Google Scholar]
  3. Asp N. G., Gudmand-Höyer E., Andersen B., Berg N. O., Dahlqvist A. Distribution of disaccharidases, alkaline phosphatase, and some intracellular enzymes along the human small intestine. Scand J Gastroenterol. 1975;10(6):647–651. [PubMed] [Google Scholar]
  4. Auricchio S., Stellato A., De Vizia B. Development of brush border peptidases in human and rat small intestine during fetal and neonatal life. Pediatr Res. 1981 Jul;15(7):991–995. doi: 10.1203/00006450-198107000-00003. [DOI] [PubMed] [Google Scholar]
  5. Dahlqvist A., Lindberg T. Development of the intestinal disaccharidase and alkaline phosphatase activities in the human foetus. Clin Sci. 1966 Jun;30(3):517–528. [PubMed] [Google Scholar]
  6. Fencl M. D., Stillman R. J., Cohen J., Tulchinsky D. Direct evidence of sudden rise in fetal corticoids late in human gestation. Nature. 1980 Sep 18;287(5779):225–226. doi: 10.1038/287225a0. [DOI] [PubMed] [Google Scholar]
  7. GAREN A., LEVINTHAL C. A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. I. Purification and characterization of alkaline phosphatase. Biochim Biophys Acta. 1960 Mar 11;38:470–483. doi: 10.1016/0006-3002(60)91282-8. [DOI] [PubMed] [Google Scholar]
  8. Grand R. J., Watkins J. B., Torti F. M. Development of the human gastrointestinal tract. A review. Gastroenterology. 1976 May;70(5 PT1):790–810. [PubMed] [Google Scholar]
  9. Haffen K., Kedinger M., Simon P. M., Raul F. Organogenetic potentialities of rat intestinal epithelioid cell culture. Differentiation. 1981;18(2):97–103. doi: 10.1111/j.1432-0436.1981.tb01109.x. [DOI] [PubMed] [Google Scholar]
  10. Haffen K., Lacroix B., Kedinger M., Simon-Assmann P. M. Inductive properties of fibroblastic cell cultures derived from rat intestinal mucosa on epithelial differentiation. Differentiation. 1983;23(3):226–233. doi: 10.1111/j.1432-0436.1982.tb01287.x. [DOI] [PubMed] [Google Scholar]
  11. Hauri H. P., Kedinger M., Haffen K., Freiburghaus A., Grenier J. F., Hadorn B. Biosynthesis of brush border glycoproteins by human small intestinal mucosa in organ culture. Biochim Biophys Acta. 1977 Jun 16;467(3):327–339. doi: 10.1016/0005-2736(77)90310-8. [DOI] [PubMed] [Google Scholar]
  12. Henning S. J. Postnatal development: coordination of feeding, digestion, and metabolism. Am J Physiol. 1981 Sep;241(3):G199–G214. doi: 10.1152/ajpgi.1981.241.3.G199. [DOI] [PubMed] [Google Scholar]
  13. Jirsová V., Koldovský O., Heringová A., Hosková J., Jirásek J., Uher J. The development of the functions of the small intestine of the human fetus. Biol Neonat. 1965;9(1):44–49. doi: 10.1159/000239976. [DOI] [PubMed] [Google Scholar]
  14. Jirsová V., Koldovský O., Heringová A., Uher J., Jodl J. Development of invertase activity in the intestines of human fetuses, appearance of jejunoileal differences. Biol Neonat. 1968;13(3):143–146. doi: 10.1159/000240141. [DOI] [PubMed] [Google Scholar]
  15. Kedinger M., Hauri H. P., Haffen K., Green J. R., Grenier J. F., Hadorn B. Turnover studies of human intestinal brush border membrane glycoproteins in organ culture. Enzyme. 1979;24(2):96–106. doi: 10.1159/000458637. [DOI] [PubMed] [Google Scholar]
  16. Kedinger M., Simon P. M., Grenier J. F., Haffen K. Role of epithelial--mesenchymal interactions in the ontogenesis of intestinal brush-border enzymes. Dev Biol. 1981 Sep;86(2):339–347. doi: 10.1016/0012-1606(81)90191-3. [DOI] [PubMed] [Google Scholar]
  17. Kedinger M., Simon P. M., Raul F., Grenier J. F., Haffen K. The effect of dexamethasone on the development of rat intestinal brush border enzymes in organ culture. Dev Biol. 1980 Jan;74(1):9–21. doi: 10.1016/0012-1606(80)90049-4. [DOI] [PubMed] [Google Scholar]
  18. Kelley R. O. An ultrastructural and cytochemical study of developing small intestine in man. J Embryol Exp Morphol. 1973 Apr;29(2):411–430. [PubMed] [Google Scholar]
  19. Koldovský O., Asp N. G., Dahlqvist A. A method for the separate assay of "neutral" and "acid" beta-galactosidase in homogenates of rat small-intestinal mucosa. Anal Biochem. 1969 Mar;27(3):409–418. doi: 10.1016/0003-2697(69)90054-2. [DOI] [PubMed] [Google Scholar]
  20. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  21. Lacroix B., Wolff-Quenot M. J., Haffen K. Early human hand morphology: an estimation of fetal age. Early Hum Dev. 1984 Feb;9(2):127–136. doi: 10.1016/0378-3782(84)90093-8. [DOI] [PubMed] [Google Scholar]
  22. Lev R., Siegel H. I., Bartman J. Histochemical studies of developing human fetal small intestine. Histochemie. 1972;29(2):103–119. doi: 10.1007/BF00277278. [DOI] [PubMed] [Google Scholar]
  23. Maroux S., Louvard D., Baratti J. The aminopeptidase from hog intestinal brush border. Biochim Biophys Acta. 1973 Sep 15;321(1):282–295. doi: 10.1016/0005-2744(73)90083-1. [DOI] [PubMed] [Google Scholar]
  24. Messer M., Dahlqvist A. A one-step ultramicro method for the assay of intestinal disaccharidases. Anal Biochem. 1966 Mar;14(3):376–392. doi: 10.1016/0003-2697(66)90280-6. [DOI] [PubMed] [Google Scholar]
  25. Moog F. Endocrine influences on the functional differentiation of the small intestine. J Anim Sci. 1979 Jul;49(1):239–249. doi: 10.2527/jas1979.491239x. [DOI] [PubMed] [Google Scholar]
  26. Moog F. Induction of brush border enzymes in organ culture. J Pediatr Gastroenterol Nutr. 1982;1(2):161–162. [PubMed] [Google Scholar]
  27. Moxey P. C., Trier J. S. Development of villus absorptive cells in the human fetal small intestine: a morphological and morphometric study. Anat Rec. 1979 Nov;195(3):463–482. doi: 10.1002/ar.1091950307. [DOI] [PubMed] [Google Scholar]
  28. Moxey P. C., Trier J. S. Specialized cell types in the human fetal small intestine. Anat Rec. 1978 Jul;191(3):269–285. doi: 10.1002/ar.1091910302. [DOI] [PubMed] [Google Scholar]
  29. Raul F., Kedinger M., Simon P. M., Grenier J. F., Haffen K. Comparative in vivo and in vitro effect of mono- and disaccharides on intestinal brush border enzyme activities in suckling rats. Biol Neonate. 1981;39(3-4):200–207. doi: 10.1159/000241437. [DOI] [PubMed] [Google Scholar]
  30. Schmitz J., Preiser H., Maestracci D., Ghosh B. K., Cerda J. J., Crane R. K. Purification of the human intestinal brush border membrane. Biochim Biophys Acta. 1973 Sep 27;323(1):98–112. doi: 10.1016/0005-2736(73)90434-3. [DOI] [PubMed] [Google Scholar]
  31. Schmitz J., Triadou N., Rey J. Développement des fonctions digestives et des mécanismes de régulation de l'appétit. Gastroenterol Clin Biol. 1978 Nov;2(11):929–940. [PubMed] [Google Scholar]
  32. Shmerling D. H. Development of digestive and absorptive function in the human fetus. Nutr Metab. 1976;20(1):76–79. doi: 10.1159/000175689. [DOI] [PubMed] [Google Scholar]
  33. Simon-Assmann P. M., Kedinger M., Grenier J. F., Haffen K. Control of brush border enzymes by dexamethasone in the fetal rat intestine cultured in vitro. J Pediatr Gastroenterol Nutr. 1982;1(2):257–265. doi: 10.1097/00005176-198201020-00017. [DOI] [PubMed] [Google Scholar]
  34. Skovbjerg H. Immunoelectrophoretic studies on human small intestinal brush border proteins--the longitudinal distribution of peptidases and disaccharidases. Clin Chim Acta. 1981 May 5;112(2):205–212. doi: 10.1016/0009-8981(81)90379-x. [DOI] [PubMed] [Google Scholar]
  35. Zweibaum A., Oriol R., Dausset J., Marcelli-Barge A., Ropartz C., Lanset S. Definition in man of a polymorphic system of the normal colonic secretions. Tissue Antigens. 1975 Sep;6(3):121–128. doi: 10.1111/j.1399-0039.1975.tb00625.x. [DOI] [PubMed] [Google Scholar]

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