Skip to main content
PMC home page
Gut logoLink to Gut
. 1984 Aug;25(8):849–853. doi: 10.1136/gut.25.8.849

Partial defect of neutrophil oxidative metabolism in Crohn's disease.

H W Verspaget, M A Mieremet-Ooms, I T Weterman, A S Peña
PMCID: PMC1432574  PMID: 6086464

Abstract

Polymorphonuclear leucocytes of patients with untreated Crohn's disease showed a lower level of oxidative metabolism than polymorphonuclear leucocytes of treated Crohn's disease patients and controls. Whereas the production of superoxide anion (O-.2) in Crohn's disease patients was almost normal, polymorphonuclear leucocytes of untreated Crohn's disease patients showed a significantly deficient production of hydrogen peroxide (H2O2). In the medically treated Crohn's disease patients, a significant negative correlation was found between H2O2 production by polymorphonuclear leucocytes and disease activity. These findings suggest an intrinsic cellular defect in the neutrophils of Crohn's disease patients which, together with the decreased locomotor function of these cells in vivo, might contribute to the pathogenesis of the chronic inflammation and granuloma formation in this disease.

Full text

PDF
849

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ament M. E., Ochs H. D. Gastrointestinal manifestations of chronic granulomatous disease. N Engl J Med. 1973 Feb 22;288(8):382–387. doi: 10.1056/NEJM197302222880802. [DOI] [PubMed] [Google Scholar]
  2. Best W. R., Becktel J. M., Singleton J. W., Kern F., Jr Development of a Crohn's disease activity index. National Cooperative Crohn's Disease Study. Gastroenterology. 1976 Mar;70(3):439–444. [PubMed] [Google Scholar]
  3. D'Amelio R., Rossi P., Le Moli S., Ricci R., Montano S., Pallone F. In vitro studies on cellular and humoral chemotaxis in Crohn's disease using the under agarose gel technique. Gut. 1981 Jul;22(7):566–570. doi: 10.1136/gut.22.7.566. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DeChatelet L. R., Shirley P. S., Johnston R. B., Jr Effect of phorbol myristate acetate on the oxidative metabolism of human polymorphonuclear leukocytes. Blood. 1976 Apr;47(4):545–554. [PubMed] [Google Scholar]
  5. Guilbault G. G., Brignac P., Jr, Zimmer M. Homovanillic acid as a fluorometric substrate for oxidative enzymes. Analytical applications of the peroxidase, glucose oxidase, and xanthine oxidase systems. Anal Chem. 1968 Jan;40(1):190–196. doi: 10.1021/ac60257a002. [DOI] [PubMed] [Google Scholar]
  6. Harvath L., Andersen B. R. Defective initiation of oxidative metabolism in polymorphonuclear leukocytes. N Engl J Med. 1979 May 17;300(20):1130–1135. doi: 10.1056/NEJM197905173002003. [DOI] [PubMed] [Google Scholar]
  7. Johnston R. B., Jr, Keele B. B., Jr, Misra H. P., Lehmeyer J. E., Webb L. S., Baehner R. L., RaJagopalan K. V. The role of superoxide anion generation in phagocytic bactericidal activity. Studies with normal and chronic granulomatous disease leukocytes. J Clin Invest. 1975 Jun;55(6):1357–1372. doi: 10.1172/JCI108055. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Moráin C. O., Segal A. A., Walker D., Levi A. J. Abnormalities of neutrophil function do not cause the migration defect in Crohn's disease. Gut. 1981 Oct;22(10):817–822. doi: 10.1136/gut.22.10.817. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Nakagawara A., Ferdais Nabi B. Z., Minakami S. An improved procedure for the diagnosis of chronic granulomatous disease, using concanavalin A and cytochalasin E. Clin Chim Acta. 1977 Jan 17;74(2):173–176. doi: 10.1016/0009-8981(77)90219-4. [DOI] [PubMed] [Google Scholar]
  10. Nathan D. G., Baehner R. L., Weaver D. K. Failure of nitro blue tetrazolium reduction in the phagocytic vacuoles of leukocytes in chronic granulomatous disease. J Clin Invest. 1969 Oct;48(10):1895–1904. doi: 10.1172/JCI106156. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Roos D., Loos J. A. Changes in the carbohydrate metabolism of mitogenically stimulated human peripheral lymphocytes. I. Stimulation by phytohaemagglutinin. Biochim Biophys Acta. 1970 Dec 29;222(3):565–582. doi: 10.1016/0304-4165(70)90182-0. [DOI] [PubMed] [Google Scholar]
  12. Rosen H., Klebanoff S. J. Bactericidal activity of a superoxide anion-generating system. A model for the polymorphonuclear leukocyte. J Exp Med. 1979 Jan 1;149(1):27–39. doi: 10.1084/jem.149.1.27. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Segal A. W., Loewi G. Neutrophil dysfunction in Crohn's disease. Lancet. 1976 Jul 31;2(7979):219–221. doi: 10.1016/s0140-6736(76)91024-2. [DOI] [PubMed] [Google Scholar]
  14. Takeshige K., Matsumoto T., Shibata R., Minakami S. Simple and rapid method for the diagnosis of chronic granulomatous disease, measuring hydrogen peroxide and superoxide anions released from leukocytes in whole blood. Clin Chim Acta. 1979 Mar 15;92(3):329–335. doi: 10.1016/0009-8981(79)90210-9. [DOI] [PubMed] [Google Scholar]
  15. Wandall J. H., Binder V. Leucocyte function in Crohn's disease. Studies on mobilisation using a quantitative skin window technique and on the function of circulating polymorphonuclear leucocytes in vitro. Gut. 1982 Mar;23(3):173–180. doi: 10.1136/gut.23.3.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ward M., Eastwood M. A. The nitroblue tetrazolium test in Crohn's disease and ulcerative colitis. Digestion. 1976;14(2):179–183. doi: 10.1159/000197924. [DOI] [PubMed] [Google Scholar]
  17. Ward M. The pathogenesis of Crohn's disease. Lancet. 1977 Oct 29;2(8044):903–905. doi: 10.1016/s0140-6736(77)90835-2. [DOI] [PubMed] [Google Scholar]
  18. Weening R. S., Roos D., Weemaes C. M., Homan-Müller J. W., van Schaik M. L. Defective initiation of the metabolic stimulation in phagocytizing granulocytes: a new congenital defect. J Lab Clin Med. 1976 Nov;88(5):757–768. [PubMed] [Google Scholar]
  19. Worsaae N., Staehr Johansen K., Christensen K. C. Impaired in vitro function of neutrophils in Crohn's disease. Scand J Gastroenterol. 1982 Jan;17(1):91–96. doi: 10.3109/00365528209181050. [DOI] [PubMed] [Google Scholar]
  20. Zabucchi G., Berton G., Soranzo M. R. Mechanism of the potentiating effect of cytochalasin B on the respiratory burst induced by concanavalin A in leucocytes. FEBS Lett. 1981 Mar 23;125(2):165–169. doi: 10.1016/0014-5793(81)80710-7. [DOI] [PubMed] [Google Scholar]

Articles from Gut are provided here courtesy of BMJ Publishing Group

RESOURCES