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. 1984 Jul;25(7):737–742. doi: 10.1136/gut.25.7.737

Subnormal activation of phagocytes by complement in chronic inflammatory bowel disease? Neutrophil chemotaxis to complement split product C5a.

J Elmgreen
PMCID: PMC1432611  PMID: 6735255

Abstract

The capacity of circulating phagocytes for activation by complement was investigated in consecutive, untreated cases of chronic inflammatory bowel disease. The major complement derived chemotactic factor, C5a, served as chemoattractant in dose response studies of neutrophil chemotaxis. A similar, significantly decreased sensitivity and peak response was revealed in patients with Crohn's disease and ulcerative colitis. This subnormal function of neutrophils could be shown even in cases of complete clinical remission. Chemotactic response to casein and spontaneous motility was within the normal range showing an unaffected basic cell function of neutrophils in the patients. The study shows a dysfunction of phagocytic cells, related to potentially important phlogistic mediators, in chronic inflammatory bowel disease.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ament M. E., Ochs H. D. Gastrointestinal manifestations of chronic granulomatous disease. N Engl J Med. 1973 Feb 22;288(8):382–387. doi: 10.1056/NEJM197302222880802. [DOI] [PubMed] [Google Scholar]
  2. Binder V. A comparison between clinical state, macroscopic and microscopic appearances of rectal mucosa, and cytologic picture of mucosal exudate in ulcerative colitis. Scand J Gastroenterol. 1970;5(7):627–632. [PubMed] [Google Scholar]
  3. Binder V., Both H., Hansen P. K., Hendriksen C., Kreiner S., Torp-Pedersen K. Incidence and prevalence of ulcerative colitis and Crohn's disease in the County of Copenhagen, 1962 to 1978. Gastroenterology. 1982 Sep;83(3):563–568. [PubMed] [Google Scholar]
  4. Chenoweth D. E., Hugli T. E. Demonstration of specific C5a receptor on intact human polymorphonuclear leukocytes. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3943–3947. doi: 10.1073/pnas.75.8.3943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Elmgreen J., Berkowicz A., Sørensen H. Defective release of C5a related chemo-attractant activity from complement in Crohn's disease. Gut. 1983 Jun;24(6):525–531. doi: 10.1136/gut.24.6.525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Elmgreen J., Berkowicz A., Sørensen H. Hypercatabolism of complement in Crohn's disease--assessment of circulating C3c. Acta Med Scand. 1983;214(5):403–407. doi: 10.1111/j.0954-6820.1983.tb08615.x. [DOI] [PubMed] [Google Scholar]
  7. Elmgreen J., Binder V. The chemotactic function of neutrophils in ulcerative colitis. Scand J Gastroenterol. 1982 Jun;17(4):561–564. doi: 10.3109/00365528209182250. [DOI] [PubMed] [Google Scholar]
  8. Goldstein I. M., Roos D., Kaplan H. B., Weissmann G. Complement and immunoglobulins stimulate superoxide production by human leukocytes independently of phagocytosis. J Clin Invest. 1975 Nov;56(5):1155–1163. doi: 10.1172/JCI108191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hodgson H. J., Potter B. J., Jewell D. P. C3 metabolism in ulcerative colitis and Crohn's disease. Clin Exp Immunol. 1977 Jun;28(3):490–495. [PMC free article] [PubMed] [Google Scholar]
  10. Lake A. M., Stitzel A. E., Urmson J. R., Walker W. A., Spitzer R. E. Complement alterations in inflammatory bowel disease. Gastroenterology. 1979 Jun;76(6):1374–1379. [PubMed] [Google Scholar]
  11. Moráin C. O., Segal A. A., Walker D., Levi A. J. Abnormalities of neutrophil function do not cause the migration defect in Crohn's disease. Gut. 1981 Oct;22(10):817–822. doi: 10.1136/gut.22.10.817. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pedersen-Bjergaard J., Vindeløv L., Philip P., Ruutu P., Elmgreen J., Repo H., Christensen I. J., Killmann S. A., Jensen G. Varying involvement of peripheral granulocytes in the clonal abnormality - 7 in bone marrow cells in preleukemia secondary to treatment of other malignant tumors: cytogenetic results compared with results of flow cytometric DNA analysis and neutrophil chemotaxis. Blood. 1982 Jul;60(1):172–179. [PubMed] [Google Scholar]
  13. Potter B. J., Brown D. J., Watson A., Jewell D. P. Complement inhibitors and immunoconglutinins in ulcerative colitis and Crohn's disease. Gut. 1980 Dec;21(12):1030–1034. doi: 10.1136/gut.21.12.1030. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rhodes J. M., Jewell D. P. Motility of neutrophils and monocytes in Crohn's disease and ulcerative colitis. Gut. 1983 Jan;24(1):73–77. doi: 10.1136/gut.24.1.73. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rhodes J. M., Potter B. J., Brown D. J., Jewell D. P. Serum inhibitors of leukocyte chemotaxis in Crohn's disease and ulcerative colitis. Gastroenterology. 1982 Jun;82(6):1327–1334. [PubMed] [Google Scholar]
  16. Segal A. W., Loewi G. Neutrophil dysfunction in Crohn's disease. Lancet. 1976 Jul 31;2(7979):219–221. doi: 10.1016/s0140-6736(76)91024-2. [DOI] [PubMed] [Google Scholar]
  17. Skubitz K. M., Craddock P. R. Reversal of hemodialysis granulocytopenia and pulmonary leukostasis: A clinical manifestation of selective down-regulation of granulocyte responses to C5adesarg. J Clin Invest. 1981 May;67(5):1383–1391. doi: 10.1172/JCI110166. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Vallota E. H., Müller-Eberhard H. J. Formation of C3a and C5a anaphylatoxins in whole human serum after inhibition of the anaphylatoxin inactivator. J Exp Med. 1973 May 1;137(5):1109–1123. doi: 10.1084/jem.137.5.1109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wandall J. H., Binder V. Leucocyte function in Crohn's disease. Studies on mobilisation using a quantitative skin window technique and on the function of circulating polymorphonuclear leucocytes in vitro. Gut. 1982 Mar;23(3):173–180. doi: 10.1136/gut.23.3.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wandall J. H., Binder V. Leucocyte function in ulcerative colitis. Quantitative leucocyte mobilisation to skin windows and in vitro function of blood leucocytes. Gut. 1982 Sep;23(9):758–765. doi: 10.1136/gut.23.9.758. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Zigmond S. H., Hirsch J. G. Leukocyte locomotion and chemotaxis. New methods for evaluation, and demonstration of a cell-derived chemotactic factor. J Exp Med. 1973 Feb 1;137(2):387–410. doi: 10.1084/jem.137.2.387. [DOI] [PMC free article] [PubMed] [Google Scholar]

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