Skip to main content
PMC home page
Gut logoLink to Gut
. 1985 May;26(5):456–460. doi: 10.1136/gut.26.5.456

Monoclonal antibody defines CA 19-9 in pancreatic juices and sera.

W H Schmiegel, C Kreiker, W Eberl, R Arndt, M Classen, H Greten, K Jessen, H Kalthoff, N Soehendra, H G Thiele
PMCID: PMC1432650  PMID: 3858206

Abstract

In a retrospective study pancreatic juice samples (n = 213) and corresponding serum samples (n = 110) were assayed for their concentration of monoclonal antibody defined CA 19-9/GICA (gastrointestinal cancer associated antigen). Serum CA 19-9 values were found to be good diagnostic and discriminating markers for pancreatic disorders and were raised (greater than 50 u/ml) in more than 80% of the pancreatic cancer patients compared to 8.5% of the pancreatitis group and none of the control group. In contrast pancreatic juice CA 19-9 values showed a considerable overlap between groups. On the basis of recent molecular data on the monoclonal antibody 19-9 defined antigen(s)--that is, monosialoganglioside, mucin--it is proposed that the discrepancies between serum and pancreatic juice findings are due to a specific undirected endocrine release of the mucin into sera in pancreatic tumour patients while in pancreatic juices of all diagnostic groups high CA 19-9 activities are either owing to coexistence of glycolipid and mucin or that the latter is a physiologically exocrine product.

Full text

PDF
456

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atkinson B. F., Ernst C. S., Herlyn M., Steplewski Z., Sears H. F., Koprowski H. Gastrointestinal cancer-associated antigen in immunoperoxidase assay. Cancer Res. 1982 Nov;42(11):4820–4823. [PubMed] [Google Scholar]
  2. Banwo O., Versey J., Hobbs J. R. New oncofetal antigen for human pancreas. Lancet. 1974 Apr;1(7859):643–645. doi: 10.1016/s0140-6736(74)93197-3. [DOI] [PubMed] [Google Scholar]
  3. Chu T. M., Holyoke E. D., Douglass H. O. Isolation of glycoprotein antigen from ascites fluid of pancreatic carcinoma. Cancer Res. 1977 May;37(5):1525–1529. [PubMed] [Google Scholar]
  4. Del Villano B. C., Brennan S., Brock P., Bucher C., Liu V., McClure M., Rake B., Space S., Westrick B., Schoemaker H. Radioimmunometric assay for a monoclonal antibody-defined tumor marker, CA 19-9. Clin Chem. 1983 Mar;29(3):549–552. [PubMed] [Google Scholar]
  5. Gelder F. B., Reese C. J., Moossa A. R., Hall T., Hunter R. Purification, partial characterization, and clinical evaluation of a pancreatic oncofetal antigen. Cancer Res. 1978 Feb;38(2):313–324. [PubMed] [Google Scholar]
  6. Herlyn M., Clark W. H., Jr, Mastrangelo M. J., Guerry D. P., 4th, Elder D. E., LaRossa D., Hamilton R., Bondi E., Tuthill R., Steplewski Z. Specific immunoreactivity of hybridoma-secreted monoclonal anti-melanoma antibodies to cultured cells and freshly derived human cells. Cancer Res. 1980 Oct;40(10):3602–3609. [PubMed] [Google Scholar]
  7. Herlyn M., Sears H. F., Steplewski Z., Koprowski H. Monoclonal antibody detection of a circulating tumor-associated antigen. I. Presence of antigen in sera of patients with colorectal, gastric, and pancreatic carcinoma. J Clin Immunol. 1982 Apr;2(2):135–140. doi: 10.1007/BF00916897. [DOI] [PubMed] [Google Scholar]
  8. Klöppel G., Sosnowski J., Eichfuss H. P., Rückert K., Klapdor R. Aktuelle Aspekte des Pankreaskarzinoms. Klinische und morphologische Analysen zur Diagnostik und Therapie. Dtsch Med Wochenschr. 1979 Dec 21;104(51):1801–1805. doi: 10.1055/s-0028-1129194. [DOI] [PubMed] [Google Scholar]
  9. Koprowski H., Herlyn M., Steplewski Z., Sears H. F. Specific antigen in serum of patients with colon carcinoma. Science. 1981 Apr 3;212(4490):53–55. doi: 10.1126/science.6163212. [DOI] [PubMed] [Google Scholar]
  10. Koprowski H., Steplewski Z., Mitchell K., Herlyn M., Herlyn D., Fuhrer P. Colorectal carcinoma antigens detected by hybridoma antibodies. Somatic Cell Genet. 1979 Nov;5(6):957–971. doi: 10.1007/BF01542654. [DOI] [PubMed] [Google Scholar]
  11. Kuntz D. J., Archer S. J. Extraction and identification of a human pancreatic-tumor-associated antigen. Oncology. 1979;36(3):134–138. doi: 10.1159/000225365. [DOI] [PubMed] [Google Scholar]
  12. Magnani J. L., Brockhaus M., Smith D. F., Ginsburg V., Blaszczyk M., Mitchell K. F., Steplewski Z., Koprowski H. A monosialoganglioside is a monoclonal antibody-defined antigen of colon carcinoma. Science. 1981 Apr 3;212(4490):55–56. doi: 10.1126/science.7209516. [DOI] [PubMed] [Google Scholar]
  13. Magnani J. L., Steplewski Z., Koprowski H., Ginsburg V. Identification of the gastrointestinal and pancreatic cancer-associated antigen detected by monoclonal antibody 19-9 in the sera of patients as a mucin. Cancer Res. 1983 Nov;43(11):5489–5492. [PubMed] [Google Scholar]
  14. Mihas A. A. Immunologic studies on a pancreatic oncofetal protein. J Natl Cancer Inst. 1978 Jun;60(6):1439–1444. doi: 10.1093/jnci/60.6.1439. [DOI] [PubMed] [Google Scholar]
  15. Raux H., Labbe F., Fondaneche M. C., Koprowski H., Burtin P. A study of gastrointestinal cancer-associated antigen (GICA) in human fetal organs. Int J Cancer. 1983 Sep 15;32(3):315–319. doi: 10.1002/ijc.2910320309. [DOI] [PubMed] [Google Scholar]
  16. Schmiegel W. H., Becker W. M., Arndt R., Hamann A., Soehendra N., Jessen K., Classen M., Thiele H. G. Pancreatic oncofetal antigen in pancreatic juices. Partial chemical characterization and diagnostic application of a pancreatic cancer-associated antigen. Scand J Gastroenterol. 1981;16(8):1033–1039. doi: 10.3109/00365528109181024. [DOI] [PubMed] [Google Scholar]
  17. Schultz D. R., Yunis A. A. Tumor-associated antigen in human pancreatic cancer. J Natl Cancer Inst. 1979 Apr;62(4):777–785. [PubMed] [Google Scholar]
  18. Sears H. F., Herlyn M., Del Villano B., Steplewski Z., Koprowski H. Monoclonal antibody detection of a circulating tumor-associated antigen. II. A longitudinal evaluation of patients with colorectal cancer. J Clin Immunol. 1982 Apr;2(2):141–149. doi: 10.1007/BF00916898. [DOI] [PubMed] [Google Scholar]

Articles from Gut are provided here courtesy of BMJ Publishing Group

RESOURCES