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. 1985 May;26(5):510–517. doi: 10.1136/gut.26.5.510

Analysis of the antigenic composition of liver specific lipoprotein using murine monoclonal antibodies.

K H Wiedmann, L K Trejdosiewicz, A H Goodall, H C Thomas
PMCID: PMC1432664  PMID: 2581852

Abstract

Eight monoclonal antibodies have been raised to antigenic determinants within the liver specific lipoprotein complex. Five of these determinants were species and the others non-species specific. No liver specific determinants were identified. Liver specific lipoprotein antigens recognised by the eight monoclonal antibodies were located either on the hepatocyte membrane and/or along the sinusoidal lining wall or in the cytoplasm of liver parenchymal cells. All antibodies but one reacted with the cell membrane of viable human hepatocytes. The molecular weights of four liver specific lipoprotein-determinants were determined by immunoprecipitation. These ranged from 22 000 to 164 000 daltons.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander J. J., Bey E. M., Geddes E. W., Lecatsas G. Establishment of a continuously growing cell line from primary carcinoma of the liver. S Afr Med J. 1976 Dec 18;50(54):2124–2128. [PubMed] [Google Scholar]
  2. Behrens U. J., Paronetto F. Studies on "liver-specific" antigens. I. Evaluation of the liver specificity of "LSP" and "LP-2". Gastroenterology. 1979 Nov;77(5):1045–1052. [PubMed] [Google Scholar]
  3. Behrens U. J., Vernace S., Paronetto F. Studies on "liver-specific" antigens. II. Detection of serum antibodies to liver and kidney cell membrane antigens in patients with chronic liver disease. Gastroenterology. 1979 Nov;77(5):1053–1061. [PubMed] [Google Scholar]
  4. Eddleston A. L., Williams R. Inadequate antibody response to hBAg or suppressor T-cell defect in development of active chronic hepatitis. Lancet. 1974 Dec 28;2(7896):1543–1545. doi: 10.1016/s0140-6736(74)90287-6. [DOI] [PubMed] [Google Scholar]
  5. Fraker P. J., Speck J. C., Jr Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphrenylglycoluril. Biochem Biophys Res Commun. 1978 Feb 28;80(4):849–857. doi: 10.1016/0006-291x(78)91322-0. [DOI] [PubMed] [Google Scholar]
  6. Galfrè G., Milstein C. Preparation of monoclonal antibodies: strategies and procedures. Methods Enzymol. 1981;73(Pt B):3–46. doi: 10.1016/0076-6879(81)73054-4. [DOI] [PubMed] [Google Scholar]
  7. Goodall A. H., Miescher G., Meek F. M., Janossy G., Thomas H. C. Monoclonal antibodies in a solid-phase radiometric assay for HBsAg. Med Lab Sci. 1981 Oct;38(4):349–354. [PubMed] [Google Scholar]
  8. Hopf U., Meyer zum Büschenfelde K. H., Arnold W. Detection of a liver-membrane autoantibody in HBsAg-negative chronic active hepatitis. N Engl J Med. 1976 Mar 11;294(11):578–582. doi: 10.1056/NEJM197603112941103. [DOI] [PubMed] [Google Scholar]
  9. Hopf U., Meyer zum Büschenfelde K. H., Freudenberg J. Liver-specific antigens of different species. II. Localization of a membrane antigen at cell surface of isolated hepatocytes. Clin Exp Immunol. 1974 Jan;16(1):117–123. [PMC free article] [PubMed] [Google Scholar]
  10. Jensen D. M., McFarlane I. G., Portmann B. S., Eddleston A. L., Williams R. Detection of antibodies directed against a liver-specific membrane lipoprotein in patients with acute and chronic active hepatitis. N Engl J Med. 1978 Jul 6;299(1):1–7. doi: 10.1056/NEJM197807062990101. [DOI] [PubMed] [Google Scholar]
  11. Kakumu S., Arakawa Y., Goji H., Kashio T., Yata K. Occurrence and significance of antibody to liver-specific membrane lipoprotein by double-antibody immunoprecipitation method in sera of patients with acute and chronic liver diseases. Gastroenterology. 1979 Apr;76(4):665–672. [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Manns M., Meyer zum Büschenfelde K. H., Hess G. Autoantibodies against liver-specific membrane lipoprotein in acute and chronic liver diseases: studies on organ-, species-, and disease-specificity. Gut. 1980 Nov;21(11):955–961. doi: 10.1136/gut.21.11.955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McFarlane I. G., Wojcicka B. M., Zucker G. M., Eddleston A. L., Williams R. Purification and characterization of human liver-specific membrane lipoprotein (LSP). Clin Exp Immunol. 1977 Mar;27(3):381–390. [PMC free article] [PubMed] [Google Scholar]
  15. Meyer zum Büschenfelde, Miescher P. A. Liver specific antigens. Purification and characterization. Clin Exp Immunol. 1972 Jan;10(1):89–102. [PMC free article] [PubMed] [Google Scholar]
  16. Miller J., Smith M. G., Mitchell C. G., Reed W. D., Eddleston A. L., Williams R. Cell-mediated immunity to a human liver-specific antigen in patients with active chronic hepatitis and primary biliary cirrhosis. Lancet. 1972 Aug 12;2(7772):296–297. doi: 10.1016/s0140-6736(72)92904-2. [DOI] [PubMed] [Google Scholar]
  17. Tsantoulas D. C., McFarlane I. G., Portmann B., Eddleston A. L., Williams R. Cell-mediated immunity to human Tamm-Horsfall glycoprotein in autoimmune liver disease with renal tubular acidosis. Br Med J. 1974 Nov 30;4(5943):491–494. doi: 10.1136/bmj.4.5943.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wiedmann K. H., Bartholemew T. C., Brown D. J., Thomas H. C. Liver membrane antibodies detected by immunoradiometric assay in acute and chronic virus-induced and autoimmune liver disease. Hepatology. 1984 Mar-Apr;4(2):199–204. doi: 10.1002/hep.1840040206. [DOI] [PubMed] [Google Scholar]

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