Skip to main content
PMC home page
Gut logoLink to Gut
. 1987 Sep;28(9):1100–1106. doi: 10.1136/gut.28.9.1100

Flow cytometric DNA analysis in longstanding ulcerative colitis: a method of prediction of dysplasia and carcinoma development?

R Löfberg 1, B Tribukait 1, A Ost 1, O Broström 1, H Reichard 1
PMCID: PMC1433223  PMID: 3678969

Abstract

During a prospective study lasting 3.5 years flow cytometric DNA analysis was evaluated as a possible predictor of dysplastic and malignant lesions in longstanding ulcerative colitis. Fifty three patients with total ulcerative colitis (mean disease duration of 22 years) were regularly colonoscoped. Biopsies of colonic mucosa were analysed by flow cytometric technique and were also assessed histologically. Findings of abnormal DNA pattern (aneuploidy) were compared with findings of dysplasia. Five patients (9%) had aneuploidy, four of those at repeated colonoscopies. Four of those patients also had various degrees of dysplasia. In one patient aneuploidy preceded the finding of dysplasia and in another aneuploidy preceded a well differentiated adenocarcinoma, grade Dukes' A subsequently found at surgery. Four additional patients had dysplasia, all in connection with macroscopic lesions, but were diploid. It is suggested that flow cytometric DNA analysis in long standing ulcerative colitis may be helpful in addition to histopathology in the detection of potential malignancy in ulcerative colitis.

Full text

PDF
1100

Images in this article

1103Fig. 3
on p.1103
1104Fig. 4
on p.1104

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Auer G., Ono J., Nasiell M., Caspersson T., Kato H., Konaka C., Hayata Y. Reversibility of bronchial cell atypia. Cancer Res. 1982 Oct;42(10):4241–4247. [PubMed] [Google Scholar]
  2. Baisch H., Göhde W., Linden W. A. Analysis of PCP-data to determine the fraction of cells in the various phases of cell cycle. Radiat Environ Biophys. 1975 Jun 13;12(1):31–39. doi: 10.1007/BF02339807. [DOI] [PubMed] [Google Scholar]
  3. Broström O., Löfberg R., Ost A., Reichard H. Cancer surveillance of patients with longstanding ulcerative colitis: a clinical, endoscopical, and histological study. Gut. 1986 Dec;27(12):1408–1413. doi: 10.1136/gut.27.12.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. De Dombal F. T., Watts J. M., Watkinson G., Goligher J. C. Local complications of ulcerative colitis: stricture, pseudopolyposis, and carcinoma of colon and rectum. Br Med J. 1966 Jun 11;1(5501):1442–1447. doi: 10.1136/bmj.1.5501.1442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. EDWARDS F. C., TRUELOVE S. C. THE COURSE AND PROGNOSIS OF ULCERATIVE COLITIS. III. COMPLICATIONS. Gut. 1964 Feb;5:1–22. doi: 10.1136/gut.5.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hammarberg C., Rubio C., Slezak P., Tribukait B., Ohman U. Flow-cytometric DNA analysis as a means for early detection of malignancy in patients with chronic ulcerative colitis. Gut. 1984 Aug;25(8):905–908. doi: 10.1136/gut.25.8.905. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hammarberg C., Slezak P., Tribukait B. Early detection of malignancy in ulcerative colitis. A flow-cytometric DNA study. Cancer. 1984 Jan 15;53(2):291–295. doi: 10.1002/1097-0142(19840115)53:2<291::aid-cncr2820530218>3.0.co;2-b. [DOI] [PubMed] [Google Scholar]
  8. Kewenter J., Ahlman H., Hultén L. Cancer risk in extensive ulcerative colitis. Ann Surg. 1978 Dec;188(6):824–828. doi: 10.1097/00000658-197812000-00018. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lennard-Jones J. E., Morson B. C., Ritchie J. K., Williams C. B. Cancer surveillance in ulcerative colitis. Experience over 15 years. Lancet. 1983 Jul 16;2(8342):149–152. doi: 10.1016/s0140-6736(83)90129-0. [DOI] [PubMed] [Google Scholar]
  10. Riddell R. H. Dysplasia and cancer in ulcerative colitis: a soluble problem? Scand J Gastroenterol Suppl. 1984;104:137–149. [PubMed] [Google Scholar]
  11. Riddell R. H., Goldman H., Ransohoff D. F., Appelman H. D., Fenoglio C. M., Haggitt R. C., Ahren C., Correa P., Hamilton S. R., Morson B. C. Dysplasia in inflammatory bowel disease: standardized classification with provisional clinical applications. Hum Pathol. 1983 Nov;14(11):931–968. doi: 10.1016/s0046-8177(83)80175-0. [DOI] [PubMed] [Google Scholar]
  12. Riddell R. H. The precarcinomatous phase of ulcerative colitis. Curr Top Pathol. 1976;63:179–219. doi: 10.1007/978-3-642-66481-6_6. [DOI] [PubMed] [Google Scholar]
  13. Tribukait B. Clinical DNA flow cytometry. Med Oncol Tumor Pharmacother. 1984;1(4):211–218. doi: 10.1007/BF02934525. [DOI] [PubMed] [Google Scholar]
  14. Tribukait B., Hammarberg C., Rubio C. Ploidy and proliferation patterns in colo-rectal adenocarcinomas related to Dukes' classification and to histopathological differentiation. A flow-cytometric DNA study. Acta Pathol Microbiol Immunol Scand A. 1983 Mar;91(2):89–95. [PubMed] [Google Scholar]

Articles from Gut are provided here courtesy of BMJ Publishing Group

RESOURCES