Abstract
We have used immunoperoxidase techniques to characterise the Peyer's patches in human terminal ileum. The mantle zones of the B cell follicles in human Peyer's patches were surrounded by B cells which did not express surface IgD but which mostly expressed surface immunoglobulin of the IgM and/or IgA1 isotype. Few cells expressing surface IgG or IgA2 were detected. Cells with cytoplasmic immunoglobulin of all isotypes except IgD were present in the dome regions of the Peyer's patches as well as in the lamina propria. There was little evidence of traffic of immunoglobulin synthesising cells across the high endothelial venules. T cells were seen to surround the lymphoid follicles. They were most concentrated on the serosal aspect around the high endothelial venules. Cells with macrophage-like morphology were present in both the lamina propria and the dome region of the follicles; those in the lamina propria containing lysozyme and those in the dome region S100 protein. The results are discussed in relation to the generation and dissemination of antibody producing cells in human gut.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cornes J. S. Number, size, and distribution of Peyer's patches in the human small intestine: Part I The development of Peyer's patches. Gut. 1965 Jun;6(3):225–229. doi: 10.1136/gut.6.3.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Crago S. S., Kutteh W. H., Moro I., Allansmith M. R., Radl J., Haaijman J. J., Mestecky J. Distribution of IgA1-, IgA2-, and J chain-containing cells in human tissues. J Immunol. 1984 Jan;132(1):16–18. [PubMed] [Google Scholar]
- Craig S. W., Cebra J. J. Peyer's patches: an enriched source of precursors for IgA-producing immunocytes in the rabbit. J Exp Med. 1971 Jul 1;134(1):188–200. doi: 10.1084/jem.134.1.188. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Curran R. C., Gregory J. Effects of fixation and processing on immunohistochemical demonstration of immunoglobulin in paraffin sections of tonsil and bone marrow. J Clin Pathol. 1980 Nov;33(11):1047–1057. doi: 10.1136/jcp.33.11.1047. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Epenetos A. A., Bobrow L. G., Adams T. E., Collins C. M., Isaacson P. G., Bodmer W. F. A monoclonal antibody that detects HLA-D region antigen in routinely fixed, wax embedded sections of normal and neoplastic lymphoid tissues. J Clin Pathol. 1985 Jan;38(1):12–17. doi: 10.1136/jcp.38.1.12. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Graham R. C., Jr, Karnovsky M. J. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966 Apr;14(4):291–302. doi: 10.1177/14.4.291. [DOI] [PubMed] [Google Scholar]
- Hall J. G., Parry D. M., Smith M. E. The distribution and differentiation of lymph-borne immunoblasts after intravenous injection into syngeneic recipients. Cell Tissue Kinet. 1972 May;5(3):269–281. doi: 10.1111/j.1365-2184.1972.tb00365.x. [DOI] [PubMed] [Google Scholar]
- Mepham B. L., Frater W., Mitchell B. S. The use of proteolytic enzymes to improve immunoglobulin staining by the PAP technique. Histochem J. 1979 May;11(3):345–357. doi: 10.1007/BF01005033. [DOI] [PubMed] [Google Scholar]
- Phillips-Quagliata J. M., Roux M. E., Arny M., Kelly-Hatfield P., McWilliams M., Lamm M. E. Migration and regulation of B-cells in the mucosal immune system. Ann N Y Acad Sci. 1983 Jun 30;409:194–203. doi: 10.1111/j.1749-6632.1983.tb26869.x. [DOI] [PubMed] [Google Scholar]
- Ralfkiaer E., Stein H., Plesner T., Hou-Jensen K., Mason D. In situ immunological characterization of Langerhans cells with monoclonal antibodies: comparison with other dendritic cells in skin and lymph nodes. Virchows Arch A Pathol Anat Histopathol. 1984;403(4):401–412. doi: 10.1007/BF00737289. [DOI] [PubMed] [Google Scholar]
- Reynolds J. D., Morris B. The effect of antigen on the development of Peyer's patches in sheep. Eur J Immunol. 1984 Jan;14(1):1–6. doi: 10.1002/eji.1830140102. [DOI] [PubMed] [Google Scholar]
- Richman L. K., Graeff A. S., Strober W. Antigen presentation by macrophage-enriched cells from the mouse Peyer's patch. Cell Immunol. 1981 Jul 15;62(1):110–118. doi: 10.1016/0008-8749(81)90304-x. [DOI] [PubMed] [Google Scholar]
- Sminia T., Plesch B. E. An immunohistochemical study of cells with surface and cytoplasmic immunoglobulins in situ in Peyer's patches and lamina propria of rat small intestine. Virchows Arch B Cell Pathol Incl Mol Pathol. 1982 Aug;40(2):181–189. doi: 10.1007/BF02932863. [DOI] [PubMed] [Google Scholar]
- Unanue E. R. The regulatory role of macrophages in antigenic stimulation. Part Two: symbiotic relationship between lymphocytes and macrophages. Adv Immunol. 1981;31:1–136. doi: 10.1016/s0065-2776(08)60919-0. [DOI] [PubMed] [Google Scholar]