Skip to main content
Gut logoLink to Gut
. 1986 Apr;27(4):386–391. doi: 10.1136/gut.27.4.386

Kinetics of lymphocyte subpopulation in intestinal mucosa of protein deficient Giardia lamblia infected mice.

P Upadhyay, N K Ganguly, B N Walia, R C Mahajan
PMCID: PMC1433403  PMID: 3485552

Abstract

Kinetics of gut lymphocyte subpopulations were studied in normal and malnourished groups of mice infected with Giardia lamblia. The maximum parasite load was observed at second, third, and first week of postinfection in normal controls, moderate (8%) and severe (3%) protein deficient groups respectively. The number of G lamblia trophozoite in 3% protein deficient group was low compared with control groups. A significant increase in T cell population of intraepithelial lymphocytes and lamina propria in normal and moderate protein deficient groups was observed with the development of infection. It was less marked, however, in the severely malnourished group. Interplay of mucosal immune status with nutrition and G lamblia infection is discussed.

Full text

PDF
386

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ament M. E., Rubin C. E. Relation of giardiasis to abnormal intestinal structure and function in gastrointestinal immunodeficiency syndromes. Gastroenterology. 1972 Feb;62(2):216–226. [PubMed] [Google Scholar]
  2. Bell R. G., Hazell L. A., Price P. Influence of dietary protein restriction on immune competence. II. Effect on lymphoid tissue. Clin Exp Immunol. 1976 Nov;26(2):314–326. [PMC free article] [PubMed] [Google Scholar]
  3. Brandborg L. L., Tankersley C. B., Gottieb S., Barancik M., Sartor V. E. Histological demonstration of mucosal invasion by Giardia lamblia in man. Gastroenterology. 1967 Feb;52(2):143–150. [PubMed] [Google Scholar]
  4. Davies M. D., Parrott D. M. Preparation and purification of lymphocytes from the epithelium and lamina propria of murine small intestine. Gut. 1981 Jun;22(6):481–488. doi: 10.1136/gut.22.6.481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Feely D. E., Erlandsen S. L. Isolation and purification of Giardia trophozoites from rat intestine. J Parasitol. 1981 Feb;67(1):59–64. [PubMed] [Google Scholar]
  6. Ferguson A., McClure J. P., Townley R. R. Intraepithelial lymphocyte counts in small intestinal biopsies from children with diarrhoea. Acta Paediatr Scand. 1976 Sep;65(5):541–546. doi: 10.1111/j.1651-2227.1976.tb04929.x. [DOI] [PubMed] [Google Scholar]
  7. Ganguly N. K., Vasudev V., Anand B. S., Chandnani R. E., Mahajan R. C. Study of lymphocyte sub-populations in Giardia infected mice. Ann Trop Med Parasitol. 1981 Jun;75(3):347–351. doi: 10.1080/00034983.1981.11687449. [DOI] [PubMed] [Google Scholar]
  8. Gracey M. Enteric disease in young Australian aborigines. Aust N Z J Med. 1973 Dec;3(6):576–579. doi: 10.1111/j.1445-5994.1973.tb04298.x. [DOI] [PubMed] [Google Scholar]
  9. Gravely S. M., Hamburger J., Kreier J. P. T and B cell population changes in young and in adult rats infected with Plasmodium berghei. Infect Immun. 1976 Jul;14(1):178–183. doi: 10.1128/iai.14.1.178-183.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jondal M., Holm G., Wigzell H. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972 Aug 1;136(2):207–215. doi: 10.1084/jem.136.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lyscom N., Brueton M. J. Intraepithelial, lamina propria and Peyer's patch lymphocytes of the rat small intestine: isolation and characterization in terms of immunoglobulin markers and receptors for monoclonal antibodies. Immunology. 1982 Apr;45(4):775–783. [PMC free article] [PubMed] [Google Scholar]
  12. MacDonald T. T., Ferguson A. Small intestinal epithelial cell kinetics and protozoal infection in mice. Gastroenterology. 1978 Mar;74(3):496–500. [PubMed] [Google Scholar]
  13. Maffei H. V., Rodrigues M. A., De Camargo J. L., Campana A. O. Intraepithelial lymphocytes in the jejunal mucosa of malnourished rats. Gut. 1980 Jan;21(1):32–36. doi: 10.1136/gut.21.1.32. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Roberts-Thomson I. C., Stevens D. P., Mahmoud A. A., Warren K. S. Giardiasis in the mouse: an animal model. Gastroenterology. 1976 Jul;71(1):57–61. [PubMed] [Google Scholar]
  15. SCRIMSHAW N. S., TAYLOR C. E., GORDON J. E. Interactions of nutrition and infection. Am J Med Sci. 1959 Mar;237(3):367–403. [PubMed] [Google Scholar]
  16. Skamene E. Antilymphocyte serum binding to cell surfaces. Fed Proc. 1970 Jan-Feb;29(1):126–127. [PubMed] [Google Scholar]
  17. Tagliabue A., Befus A. D., Clark D. A., Bienenstock J. Characteristics of natural killer cells in the murine intestinal epithelium and lamina propria. J Exp Med. 1982 Jun 1;155(6):1785–1796. doi: 10.1084/jem.155.6.1785. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Upadhyay P., Ganguly N. K., Mahajan R. C., Walia B. N. Intestinal uptake of nutrients in normal and malnourished animals infected with Giardia lamblia. Digestion. 1985;32(4):243–248. doi: 10.1159/000199244. [DOI] [PubMed] [Google Scholar]
  19. Wright S. G., Tomkins A. M. Quantification of the lymphocytic infiltrate in jejunal epithelium in giardiasis. Clin Exp Immunol. 1977 Sep;29(3):408–412. [PMC free article] [PubMed] [Google Scholar]

Articles from Gut are provided here courtesy of BMJ Publishing Group

RESOURCES