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. 1986 Jul;27(7):765–770. doi: 10.1136/gut.27.7.765

Disorganisation of intermediate filament structure in alcoholic and other liver diseases.

C Barbatis, J Morton, J C Woods, J Burns, J Bradley, J O McGee
PMCID: PMC1433570  PMID: 3732887

Abstract

The distribution of Mallory body antigens JMB1 and 2 was examined in 82 human fresh diagnostic needle liver biopsies and 28 necropsies by the indirect immunoperoxidase technique using 2 monoclonal antibodies (anti-JMB1 and 2) against Mallory bodies. The JMB1 antigen was detectable in bile duct epithelium and in hepatocytes of histologically normal livers. It was also found in all Mallory bodies in various hepatic disorders. This antigen was markedly increased in the cytoplasm of all liver cells in acute alcoholic hepatitis superimposed on alcoholic cirrhosis, in most cases of acute alcoholic hepatitis, and in severe fatty infiltration of the liver with or without Mallory body formation. Mallory bodies contained this antigen but the cytoplasm of Mallory body containing cells lacked JMB1. In normal liver the JMB2 antigen was localised on the cytoplasmic intermediate filament network of hepatocytes and bile duct epithelium; and almost all Mallory bodies also contained this antigen but the adjacent cytoplasm of these cells lacked JMB2. In severe alcoholic liver disease these antigens could not be detected in large zones of hepatocytes even when these hepatocytes did not contain Mallory bodies. It is evident that there is disorganisation of intermediate filament constituents in severe alcoholic liver disease.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baraona E., Leo M. A., Borowsky S. A., Lieber C. S. Pathogenesis of alcohol-induced accumulation of protein in the liver. J Clin Invest. 1977 Sep;60(3):546–554. doi: 10.1172/JCI108806. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barbatis C., Woods J., Morton J. A., Fleming K. A., McMichael A., McGee J. O. Immunohistochemical analysis of HLA (A, B, C) antigens in liver disease using a monoclonal antibody. Gut. 1981 Dec;22(12):985–991. doi: 10.1136/gut.22.12.985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Borenfreund E., Higgins P. J., Peterson E. Intermediate-sized filaments in cultured rat liver tumor cells with Mallory body-like cytoplasm abnormalities. J Natl Cancer Inst. 1980 Feb;64(2):323–333. doi: 10.1093/jnci/64.2.323. [DOI] [PubMed] [Google Scholar]
  4. Brodsky F. M., Parham P., Barnstable C. J., Crumpton M. J., Bodmer W. F. Monoclonal antibodies for analysis of the HLA system. Immunol Rev. 1979;47:3–61. doi: 10.1111/j.1600-065x.1979.tb00288.x. [DOI] [PubMed] [Google Scholar]
  5. DeBrabander M., Aerts F., Van de Veire R., Borgers M. Evidence against interconversion of microtubules and filaments. Nature. 1975 Jan 10;253(5487):119–120. doi: 10.1038/253119a0. [DOI] [PubMed] [Google Scholar]
  6. Denk H., Eckerstorfer R. Colchicine-induced Mallory body formation in the mouse. Lab Invest. 1977 Jun;36(6):563–565. [PubMed] [Google Scholar]
  7. Denk H., Eckerstorfer R. Colchicine-induced Mallory body formation in the mouse. Lab Invest. 1977 Jun;36(6):563–565. [PubMed] [Google Scholar]
  8. Denk H., Franke W. W., Dragosics B., Zeiler I. Pathology of cytoskeleton of liver cells: demonstration of mallory bodies (alcoholic hyalin) in murine and human hepatocytes by immunofluorescence microscopy using antibodies to cytokeratin polypeptides from hepatocytes. Hepatology. 1981 Jan-Feb;1(1):9–20. doi: 10.1002/hep.1840010103. [DOI] [PubMed] [Google Scholar]
  9. Denk H., Franke W. W., Eckerstorfer R., Schmid E., Kerjaschki D. Formation and involution of Mallory bodies ("alcoholic hyalin") in murine and human liver revealed by immunofluorescence microscopy with antibodies to prekeratin. Proc Natl Acad Sci U S A. 1979 Aug;76(8):4112–4116. doi: 10.1073/pnas.76.8.4112. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Edmondson H. A., Peters R. L., Frankel H. H., Borowsky S. The early stage of liver injury in the alcoholic. Medicine (Baltimore) 1967 Mar;46(2):119–129. doi: 10.1097/00005792-196703000-00006. [DOI] [PubMed] [Google Scholar]
  11. French S. W. The Mallory body: structure, composition, and pathogenesis. Hepatology. 1981 Jan-Feb;1(1):76–83. doi: 10.1002/hep.1840010113. [DOI] [PubMed] [Google Scholar]
  12. Galambos J. T. Natural history of alcoholic hepatitis. 3. Histological changes. Gastroenterology. 1972 Dec;63(6):1026–1035. [PubMed] [Google Scholar]
  13. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  14. Lazarides E. Intermediate filaments as mechanical integrators of cellular space. Nature. 1980 Jan 17;283(5744):249–256. doi: 10.1038/283249a0. [DOI] [PubMed] [Google Scholar]
  15. Lischner M. W., Alexander J. F., Galambos J. T. Natural history of alcoholic hepatitis. I. The acute disease. Am J Dig Dis. 1971 Jun;16(6):481–494. doi: 10.1007/BF02235538. [DOI] [PubMed] [Google Scholar]
  16. Matsuda Y., Baraona E., Salaspuro M., Lieber C. S. Effects of ethanol on liver microtubules and Golgi apparatus. Possible role in altered hepatic secretion of plasma proteins. Lab Invest. 1979 Nov;41(5):455–463. [PubMed] [Google Scholar]
  17. Morgan M. Y., Sherlock S. Sex-related differences among 100 patients with alcoholic liver disease. Br Med J. 1977 Apr 9;1(6066):939–941. doi: 10.1136/bmj.1.6066.939. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Morton J. A., Bastin J., Fleming K. A., McMichael A., Burns J., McGee J. O. Mallory bodies in alcoholic liver disease: identification of cytoplasmic filament/cell membrane and unique antigenic determinants by monoclonal antibodies. Gut. 1981 Jan;22(1):1–7. doi: 10.1136/gut.22.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Popper H., Lieber C. S. Histogenesis of alcoholic fibrosis and cirrhosis in the baboon. Am J Pathol. 1980 Mar;98(3):695–716. [PMC free article] [PubMed] [Google Scholar]
  20. Rumpelt H. J. Ultrastructure of alcoholic hyalin and fate of the affected hepatocytes. Virchows Arch B Cell Pathol. 1977 Apr 15;23(4):339–351. doi: 10.1007/BF02889142. [DOI] [PubMed] [Google Scholar]
  21. Shatkin A. J., Darzynkiewicz E., Furuichi Y., Kroath H., Morgan M. A., Tahara S. M., Yamakawa M. 5'-Terminal caps, cap-binding proteins and eukaryotic mRNA function. Biochem Soc Symp. 1982;47:129–143. [PubMed] [Google Scholar]
  22. Sorrell M. F., Leevy C. M. Lymphocyte transformation and alcoholic liver injury. Gastroenterology. 1972 Dec;63(6):1020–1025. [PubMed] [Google Scholar]
  23. Van Waes L., Lieber C. S. Early perivenular sclerosis in alcoholic fatty liver: an index of progressive liver injury. Gastroenterology. 1977 Oct;73(4 Pt 1):646–650. [PubMed] [Google Scholar]
  24. Wiggers K. D., French S. W., French B. A., Carr B. N. The ultrastructure of Mallory body filaments. Lab Invest. 1973 Dec;29(6):652–658. [PubMed] [Google Scholar]
  25. Zetterman R. K., Luisada-Opper A., Leevy C. M. Alcoholic hepatitis. Cell-mediated immunological response to alcoholic hyalin. Gastroenterology. 1976 Mar;70(3):382–384. [PubMed] [Google Scholar]
  26. Zumbé A., Stähli C., Trachsel H. Association of a Mr 50,000 cap-binding protein with the cytoskeleton in baby hamster kidney cells. Proc Natl Acad Sci U S A. 1982 May;79(9):2927–2931. doi: 10.1073/pnas.79.9.2927. [DOI] [PMC free article] [PubMed] [Google Scholar]

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