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. 1988 May;29(5):642–646. doi: 10.1136/gut.29.5.642

Effects of fasting and refeeding on somatostatin concentration and binding to cytosol from rabbit gastric mucosa.

B Roca 1, R Fernandez-Valencia 1, E Arilla 1
PMCID: PMC1433626  PMID: 2899535

Abstract

Somatostatin like immunoreactivity and the density of somatostatin binding sites were measured in stomach (fundus and antrum) from either fed, 12 to 96 hours fasted, or 96 hours fasted plus 48 hours refed rabbits. The somatostatin concentration increased in fundic and antral mucosa after 24 h and reached its highest value after 96 h of fasting. The number of specific somatostatin binding sites with high and low affinity decreased with the duration of fasting. Refeeding of fasted animals resulted in a normalisation to control values of gastric mucosal somatostatin and somatostatin binding.

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Selected References

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  1. Alumets J., Ekelund M., El Munshid H. A., Håkanson R., Lorén I., Sundler F. Topography of somatostatin cells in the stomach of the rat: possible functional significance. Cell Tissue Res. 1979 Nov;202(2):177–188. doi: 10.1007/BF00232233. [DOI] [PubMed] [Google Scholar]
  2. Arilla E., Lopez-Ruiz M. P., Gonzalez Guijarro L., Prieto J. C., Gomez-Pan A., Hirst B. Characterization of somatostatin binding sites in cytosolic fraction of rat intestinal mucosa. Biochim Biophys Acta. 1984 Nov 28;802(2):203–208. doi: 10.1016/0304-4165(84)90162-4. [DOI] [PubMed] [Google Scholar]
  3. Conlon J. M., Whittaker J., Hammond V., Alberti K. G. Metabolism of somatostatin and its analogues by the liver. Biochim Biophys Acta. 1981 Oct 12;677(2):234–242. doi: 10.1016/0304-4165(81)90090-8. [DOI] [PubMed] [Google Scholar]
  4. DeMeyts P., Kahn C. R., Roth J., Bar R. S. Hormonal regulation of the affinity and concentration of hormone receptors in target cells. Metabolism. 1976 Nov;25(11 Suppl 1):1365–1370. doi: 10.1016/s0026-0495(76)80143-6. [DOI] [PubMed] [Google Scholar]
  5. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gerich J., Greene K., Hara M., Rizza R., Patton G. Radioimmunoassay of somatostatin and its application in the study of pancreatic somatostatin secretion in vitro. J Lab Clin Med. 1979 Jun;93(6):1009–1017. [PubMed] [Google Scholar]
  7. Guijarro L. G., Arilla E., Lopez-Ruiz M. P., Prieto J. C., Whitford C., Hirst B. H. Somatostatin binding sites in cytosolic fraction isolated from rabbit antral and fundic gastric mucosa. Regul Pept. 1985 Mar;10(2-3):207–215. doi: 10.1016/0167-0115(85)90015-1. [DOI] [PubMed] [Google Scholar]
  8. Hinkle P. M., Tashjian A. H., Jr Thyrotropin-releasing hormone regulates the number of its own receptors in the GH3 strain of pituitary cells in culture. Biochemistry. 1975 Aug 26;14(17):3845–3851. doi: 10.1021/bi00688a017. [DOI] [PubMed] [Google Scholar]
  9. Koop H., Schwab E., Arnold R., Creutzfeldt W. Effect of food deprivation on rat gastric somatostatin and gastrin release. Gastroenterology. 1982 May;82(5 Pt 1):871–876. [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Lesniak M. A., Roth J. Regulation of receptor concentration by homologous hormone. Effect of human growth hormone on its receptor in IM-9 lymphocytes. J Biol Chem. 1976 Jun 25;251(12):3720–3729. [PubMed] [Google Scholar]
  12. Majumdar A. P. Regulation of gastric mucosal DNA synthesis during fasting and refeeding in rats. Digestion. 1983;27(1):36–43. doi: 10.1159/000198917. [DOI] [PubMed] [Google Scholar]
  13. Mukherjee C., Caron M. G., Lefkowitz R. J. Catecholamine-induced subsensitivity of adenylate cyclase associated with loss of beta-adrenergic receptor binding sites. Proc Natl Acad Sci U S A. 1975 May;72(5):1945–1949. doi: 10.1073/pnas.72.5.1945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. NISSIM J. A. Gastrointestinal lesions in starvation induced by drugs or simple food reduction. Lancet. 1961 Jul 15;2(7194):132–135. doi: 10.1016/s0140-6736(61)92650-2. [DOI] [PubMed] [Google Scholar]
  15. Ogawa N., Thompson T., Friesen H. G., Martin J. B., Brazeau P. Properties of soluble somatostatin-binding protein. Biochem J. 1977 Aug 1;165(2):269–277. doi: 10.1042/bj1650269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Palmblad J., Levi L., Burger A., Melander A., Westgren U., von Schenck H., Skude G. Effects of total energy withdrawal (fasting) on thelevels of growth hormone, thyrotropin, cortisol, adrenaline, noradrenaline, T4, T3, and rT3 in healthy males. Acta Med Scand. 1977 Jan;201(1-2):15–22. doi: 10.1111/j.0954-6820.1977.tb15648.x. [DOI] [PubMed] [Google Scholar]
  17. Reyl-Desmars F., Lewin M. J. Evidence for an intracellular somatostatin receptor in pancreas: a comparative study with reference to gastric mucosa. Biochem Biophys Res Commun. 1982 Dec 31;109(4):1324–1331. doi: 10.1016/0006-291x(82)91922-2. [DOI] [PubMed] [Google Scholar]
  18. Reyl F. J., Lewin M. J. Intracellular receptor for somatostatin in gastric mucosal cells: decomposition and reconstitution of somatostatin-stimulated phosphoprotein phosphatases. Proc Natl Acad Sci U S A. 1982 Feb;79(4):978–982. doi: 10.1073/pnas.79.4.978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schonbrunn A. Glucocorticoids down-regulate somatostatin receptors on pituitary cells in culture. Endocrinology. 1982 Apr;110(4):1147–1154. doi: 10.1210/endo-110-4-1147. [DOI] [PubMed] [Google Scholar]
  20. Schwarting H., Koop H., Gellert G., Arnold R. Effect of starvation on endocrine cells in the rat stomach. Regul Pept. 1986 Mar;14(1):33–39. doi: 10.1016/0167-0115(86)90203-x. [DOI] [PubMed] [Google Scholar]
  21. Shulkes A., Caussignac Y., Lamers C. B., Solomon T. E., Yamada T., Walsh J. H. Starvation in the rat: effect on peptides of the gut and brain. Aust J Exp Biol Med Sci. 1983 Oct;61(Pt 5):581–587. doi: 10.1038/icb.1983.55. [DOI] [PubMed] [Google Scholar]
  22. Szabo S., Usadel K. H. Cytoprotection - organoprotection by somatostatin: gastric and hepatic lesions. Experientia. 1982 Feb 15;38(2):254–256. doi: 10.1007/BF01945097. [DOI] [PubMed] [Google Scholar]
  23. Tanigawa K., Kuzuya H., Seino Y., Seino S., Tsuda K., Sakurai H., Imura H. Effect of starvation on somatostatin content of pancreas and gastrointestinal tract of the guinea pig. Horm Metab Res. 1982 May;14(5):245–247. doi: 10.1055/s-2007-1018983. [DOI] [PubMed] [Google Scholar]
  24. Tannenbaum G. S., Rorstad O., Brazeau P. Effects of prolonged food deprivation on the ultradian growth hormone rhythm and immunoreactive somatostatin tissue levels in the rat. Endocrinology. 1979 Jun;104(6):1733–1738. doi: 10.1210/endo-104-6-1733. [DOI] [PubMed] [Google Scholar]
  25. Voyles N. R., Awoke S., Wade A., Bhathena S. J., Smith S. S., Recant L. Starvation increases gastrointestinal somatostatin in normal and obese Zucker rats: a possible regulatory mechanism. Horm Metab Res. 1982 Aug;14(8):392–395. doi: 10.1055/s-2007-1019027. [DOI] [PubMed] [Google Scholar]

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