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. 1988 May;29(5):580–587. doi: 10.1136/gut.29.5.580

Lactosylceramide in inflammatory bowel disease: a biochemical study.

C R Stevens 1, V G Oberholzer 1, J A Walker-Smith 1, A D Phillips 1
PMCID: PMC1433632  PMID: 3396945

Abstract

A simple technique for isolating lipids from small pieces of tissue was applied to mucosal biopsies and samples of resected intestine from patients with inflammatory bowel disease. Scanning densitometry of two dimensional chromatograms showed increased concentrations of the membrane associated glycosphingolipid lactosylceramide in Crohn's disease, on comparison with ulcerative colitis (p less than 0.01), or controls (p less than 0.01). This indicates either that normal glycosphingolipid metabolism is altered, or that a novel source of lactosylceramide is present in the inflamed mucosa of patients with Crohn's disease.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. Bouhours J. F., Glickman R. M. Rat intestinal glycolipids. II. Distribution and biosynthesis of glycolipids and ceramide in villus and crypt cells. Biochim Biophys Acta. 1976 Jul 20;441(1):123–133. doi: 10.1016/0005-2760(76)90287-3. [DOI] [PubMed] [Google Scholar]
  3. Chatterjee S., Clarke K. S., Kwiterovich P. O., Jr Uptake and metabolism of lactosylceramide on low density lipoproteins in cultured proximal tubular cells from normal and familial hypercholesterolemic homozygotes. J Biol Chem. 1986 Oct 15;261(29):13480–13486. [PubMed] [Google Scholar]
  4. Chong S. K., Bartram C., Campbell C. A., Williams C. B., Blackshaw A. J., Walker-Smith J. A. Chronic inflammatory bowel disease in childhood. Br Med J (Clin Res Ed) 1982 Jan 9;284(6309):101–103. doi: 10.1136/bmj.284.6309.101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chong S. K., Blackshaw A. J., Boyle S., Williams C. B., Walker-Smith J. A. Histological diagnosis of chronic inflammatory bowel disease in childhood. Gut. 1985 Jan;26(1):55–59. doi: 10.1136/gut.26.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Den H., Sela B. A., Roseman S., Sachs L. Blocks in ganglioside synthesis in transformed hamster cells and their revertants. J Biol Chem. 1974 Jan 25;249(2):659–661. [PubMed] [Google Scholar]
  7. Ferrante A., James D. W., Betts W. H., Cleland L. G. Rapid single-step method for purification of polymorphonuclear leucocytes from blood of patients with rheumatoid arthritis. Clin Exp Immunol. 1982 Mar;47(3):749–752. [PMC free article] [PubMed] [Google Scholar]
  8. Fukuda M. N., Dell A., Oates J. E., Wu P., Klock J. C., Fukuda M. Structures of glycosphingolipids isolated from human granulocytes. The presence of a series of linear poly-N-acetyllactosaminylceramide and its significance in glycolipids of whole blood cells. J Biol Chem. 1985 Jan 25;260(2):1067–1082. [PubMed] [Google Scholar]
  9. Glickman R. M., Bouhours J. F. Characterization, distribution and biosynthesis of the major ganglioside of rat intestinal mucosa. Biochim Biophys Acta. 1976 Jan 22;424(1):17–25. doi: 10.1016/0005-2760(76)90045-x. [DOI] [PubMed] [Google Scholar]
  10. Glotzer D. J. The risk of cancer in Crohn's disease. Gastroenterology. 1985 Aug;89(2):438–441. doi: 10.1016/0016-5085(85)90350-6. [DOI] [PubMed] [Google Scholar]
  11. Hakomori S. I., Murakami W. T. Glycolipids of hamster fibroblasts and derived malignant-transformed cell lines. Proc Natl Acad Sci U S A. 1968 Jan;59(1):254–261. doi: 10.1073/pnas.59.1.254. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hakomori S. Glycosphingolipids in cellular interaction, differentiation, and oncogenesis. Annu Rev Biochem. 1981;50:733–764. doi: 10.1146/annurev.bi.50.070181.003505. [DOI] [PubMed] [Google Scholar]
  13. Heymans H. S., vd Bosch H., Schutgens R. B., Tegelaers W. H., Walther J. U., Müller-Höcker J., Borst P. Deficiency of plasmalogens in the cerebro-hepato-renal (Zellweger) syndrome. Eur J Pediatr. 1984 Apr;142(1):10–15. doi: 10.1007/BF00442582. [DOI] [PubMed] [Google Scholar]
  14. Kanazawa I., Yamakawa T. Presence of glucosyl ceramide and lactosyl ceramide in human intracranial tumours. Jpn J Exp Med. 1974 Oct;44(5):379–387. [PubMed] [Google Scholar]
  15. Kane S. P., Hoffbrand A. V., Neale G. Indices of granulocyte activity in inflammatory bowel disease. Gut. 1974 Dec;15(12):953–959. doi: 10.1136/gut.15.12.953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. LOCKHART-MUMMERY H. E., MORSON B. C. Crohn's disease (regional enteritis) of the large intestine and its distinction from ulcerative colitis. Gut. 1960 Jun;1:87–105. doi: 10.1136/gut.1.2.87. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lewis D. C., Walker-Smith J. A., Phillips A. D. Polymorphonuclear neutrophil leucocytes in childhood Crohn's disease: a morphological study. J Pediatr Gastroenterol Nutr. 1987 May-Jun;6(3):430–438. doi: 10.1097/00005176-198705000-00021. [DOI] [PubMed] [Google Scholar]
  18. Lewis D., Walker-Smith J. A., Phillips A. D. Microvilli- and desmosome-associated bodies in Crohn's disease and other disorders in childhood: an ultrastructural abnormality of the small and large intestine. J Pediatr Gastroenterol Nutr. 1984;3(1):46–55. doi: 10.1097/00005176-198401000-00012. [DOI] [PubMed] [Google Scholar]
  19. Macher B. A., Klock J. C. Isolation and chemical characterization of neutral glycosphingolipids of human neutrophils. J Biol Chem. 1980 Mar 10;255(5):2092–2096. [PubMed] [Google Scholar]
  20. Otto H. F., Gebbers J. O. Electron microscopic, ultracytochemical and immunohistological observations in Crohn's disease of the ileum and colon. Virchows Arch A Pathol Anat Histol. 1981;391(2):189–205. doi: 10.1007/BF00437596. [DOI] [PubMed] [Google Scholar]
  21. Owens K. A two-dimensional thin-layer chromatographic procedure for the estimation of plasmalogens. Biochem J. 1966 Aug;100(2):354–361. doi: 10.1042/bj1000354. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rouser G., Fkeischer S., Yamamoto A. Two dimensional then layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots. Lipids. 1970 May;5(5):494–496. doi: 10.1007/BF02531316. [DOI] [PubMed] [Google Scholar]
  23. Rutgeerts P., Ghoos Y., Vantrappen G., Fevery J. Biliary lipid composition in patients with nonoperated Crohn's disease. Dig Dis Sci. 1986 Jan;31(1):27–32. doi: 10.1007/BF01347906. [DOI] [PubMed] [Google Scholar]
  24. Saverymuttu S. H., Peters A. M., Lavender J. P., Hodgson H. J., Chadwick V. S. 111Indium autologous leucocytes in inflammatory bowel disease. Gut. 1983 Apr;24(4):293–299. doi: 10.1136/gut.24.4.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Siddiqui B., Whitehead J. S., Kim Y. S. Glycosphingolipids in human colonic adenocarcinoma. J Biol Chem. 1978 Apr 10;253(7):2168–2175. [PubMed] [Google Scholar]
  26. Vanier M. T. Biochemical studies in Niemann-Pick disease. I. Major sphingolipids of liver and spleen. Biochim Biophys Acta. 1983 Jan 7;750(1):178–184. doi: 10.1016/0005-2760(83)90218-7. [DOI] [PubMed] [Google Scholar]

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