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. 1988 Aug;29(8):1093–1097. doi: 10.1136/gut.29.8.1093

Cytosolic retinoic acid binding protein in the human pancreas.

J K Jutley 1, J Kelleher 1, T G Brennan 1, M E Denyer 1, C J Mitchell 1
PMCID: PMC1433899  PMID: 2842235

Abstract

Cytosolic retinoic acid receptor in carcinoma, chronic pancreatitis, and normal pancreatic tissue were examined using sucrose density gradient centrifugation, isoelectric focussing on agarose gel and saturation analysis. Thirteen patients were studied. Cytosolic retinoic acid binding protein (cRABP) was detected in all the samples with chronic pancreatitis and pancreatic carcinoma, but not in the normal tissue. Using sucrose gradient centrifugation, the highest concentrations of cRABP were found in pancreatic carcinoma tissues, ranging from 5.5-23.9 pmol/mg protein. These concentrations were markedly different than in chronic pancreatitis tissue (0.7-2.7 pmol/mg protein). Saturation analysis of cRABP showed a mean dissociation constant of 21.5 nM and maximum binding sites of 5.2 pmol/mg protein. Cytosolic retinoic acid binding protein was separated at an isoelectric point of 4.5 on agarose gel. The presence of cRABP suggest that retinoic acid may have a role to play in the function of the pancreas.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bichler E., Daxenbichler G. Retinoic acid-binding protein in human squamous cell carcinomas of the ORL region. Cancer. 1982 Feb 15;49(4):619–622. doi: 10.1002/1097-0142(19820215)49:4<619::aid-cncr2820490402>3.0.co;2-v. [DOI] [PubMed] [Google Scholar]
  2. Boyd D., Beynon L., Chisholm G. D., Habib F. K. Characterization of the retinol and retinoic acid binding proteins in the human prostate. Cancer Res. 1984 Dec;44(12 Pt 1):5532–5537. [PubMed] [Google Scholar]
  3. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  4. Chytil F., Ong D. E. Cellular retinol- and retinoic acid-binding proteins in vitamin A action. Fed Proc. 1979 Oct;38(11):2510–2514. [PubMed] [Google Scholar]
  5. Clamon G. H., Nugent K. M., Rossi N. P. Cellular retinoic acid-binding protein in human lung carcinomas. J Natl Cancer Inst. 1981 Jul;67(1):61–63. [PubMed] [Google Scholar]
  6. Davies R. E. Effect of vitamin A on 7,12-Dimethylbenz(alpha)anthracene-induced papillomas in rhino mouse skin. Cancer Res. 1967 Feb;27(2):237–241. [PubMed] [Google Scholar]
  7. Fagg S. L., Hughes A., Fielding J. W., Dawson-Edwards P., Hughes M. A., Howie A. Retinoic acid receptor in human bladder tumours. Clin Oncol. 1982 Dec;8(4):329–334. [PubMed] [Google Scholar]
  8. Grubbs C. J., Moon R. C., Sporn M. B., Newton D. L. Inhibition of mammary cancer by retinyl methyl ether. Cancer Res. 1977 Feb;37(2):599–602. [PubMed] [Google Scholar]
  9. Jutley J. K., Kelleher J., Whelan P., Mikel J. Cytosolic retinoic acid-binding protein in human prostatic dysplasia and neoplasia. Prostate. 1987;11(2):127–132. doi: 10.1002/pros.2990110204. [DOI] [PubMed] [Google Scholar]
  10. Küng W. M., Geyer E., Eppenberger U., Huber P. R. Quantitative estimation of cellular retinoic acid-binding protein activity in normal, dysplastic, and neoplastic human breast tissue. Cancer Res. 1980 Nov;40(11):4265–4269. [PubMed] [Google Scholar]
  11. LASNITZKI I. GROWTH PATTERN OF THE MOUSE PROSTATE GLAND IN ORGAN CULTURE AND ITS RESPONSE TO SEX HORMONES, VITAMIN A, AND 3-METHYLCHOLANTHRENE. Natl Cancer Inst Monogr. 1963 Oct;12:381–403. [PubMed] [Google Scholar]
  12. Moon R. C., Grubbs C. J., Sporn M. B., Goodman D. G. Retinyl acetate inhibits mammary carcinogenesis induced by N-methyl-N-nitrosourea. Nature. 1977 Jun 16;267(5612):620–621. doi: 10.1038/267620a0. [DOI] [PubMed] [Google Scholar]
  13. Murtaugh M. P., Dennison O., Stein J. P., Davies P. J. Retinoic acid-induced gene expression in normal and leukemic myeloid cells. J Exp Med. 1986 May 1;163(5):1325–1330. doi: 10.1084/jem.163.5.1325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ong D. E., Chytil F. Multiple retinol binding proteins in rabbit lung. Biochem Biophys Res Commun. 1974 Jul 10;59(1):221–229. doi: 10.1016/s0006-291x(74)80196-8. [DOI] [PubMed] [Google Scholar]
  15. Ong D. E., Goodwin W. J., Jesse R. H., Griffin A. C. Presence of cellular retinol and retinoic acid-binding proteins in epidermoid carcinoma of the oral cavity and oropharynx. Cancer. 1982 Apr 1;49(7):1409–1412. doi: 10.1002/1097-0142(19820401)49:7<1409::aid-cncr2820490717>3.0.co;2-o. [DOI] [PubMed] [Google Scholar]
  16. Ong D. E., Page D. L., Chytil F. Retinoic acid binding protein: occurrence in human tumors. Science. 1975 Oct 3;190(4209):60–61. doi: 10.1126/science.1166300. [DOI] [PubMed] [Google Scholar]
  17. Palan P. R., Romney S. L. Cellular binding proteins for vitamin A in human carcinomas and in normal tissues. Cancer Res. 1980 Nov;40(11):4221–4224. [PubMed] [Google Scholar]
  18. Sani B. P. Localization of retinoic acid-binding protein in nuclei. Biochem Biophys Res Commun. 1977 Mar 7;75(1):7–12. doi: 10.1016/0006-291x(77)91281-5. [DOI] [PubMed] [Google Scholar]
  19. Schwartz J. L., Frim S. R., Shklar G. RA can alter the distribution of ATPase-positive Langerhans cells in the hamster cheek pouch in association with DMBA application. Nutr Cancer. 1985;7(1-2):77–84. doi: 10.1080/01635588509513842. [DOI] [PubMed] [Google Scholar]
  20. Siegenthaler G., Saurat J. H. A slab gel electrophoresis technique for measurement of plasma retinol-binding protein, cellular retinol-binding and retinoic-acid-binding proteins in human skin. Eur J Biochem. 1987 Jul 1;166(1):209–214. doi: 10.1111/j.1432-1033.1987.tb13503.x. [DOI] [PubMed] [Google Scholar]
  21. Sporn M. B., Dunlop N. M., Newton D. L., Smith J. M. Prevention of chemical carcinogenesis by vitamin A and its synthetic analogs (retinoids). Fed Proc. 1976 May 1;35(6):1332–1338. [PubMed] [Google Scholar]
  22. Thaller C., Eichele G. Identification and spatial distribution of retinoids in the developing chick limb bud. Nature. 1987 Jun 18;327(6123):625–628. doi: 10.1038/327625a0. [DOI] [PubMed] [Google Scholar]
  23. Trown P. W., Buck M. J., Hansen R. Inhibition of growth and regression of a transplantable rat chondrosarcoma by three retinoids. Cancer Treat Rep. 1976 Nov;60(11):1647–1653. [PubMed] [Google Scholar]

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