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. 1989 May;30(5):600–604. doi: 10.1136/gut.30.5.600

Effects of gastrointestinal hormones on the growth of human intestinal epithelial cells in vitro.

C Simopoulos 1, J D Gaffen 1, A Bennett 1
PMCID: PMC1434197  PMID: 2731751

Abstract

The growth of cultured epithelium like cells from human normal embryonic intestine was studied in response to various hormones using a method that quantifies the number of cells by the amount of dye that they bind after fixation. Gastrin and neurotensin in the pg/ml range and higher caused small increases in cell growth. Glucagon and VIP were stimulatory in the low ng/ml range, whereas somatostatin and bombesin had no effect at the lower concentrations but were stimulatory at the highest concentration tested (10 and 100 ng/ml respectively). Secretin and pancreozymin (cholecystokinin) seemed to be ineffective.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aynsley-Green A. The role of regulatory peptides in postnatal metabolic adaptation. Acta Endocrinol Suppl (Copenh) 1986;279:422–427. doi: 10.1530/acta.0.112s422. [DOI] [PubMed] [Google Scholar]
  2. Barer M. R., Lyon H., Drasar B. S. Quantitation of dye binding by cell monolayers in a microtiter system. Histochem J. 1986 Feb-Mar;18(2-3):122–128. doi: 10.1007/BF01675366. [DOI] [PubMed] [Google Scholar]
  3. Buts J. P., De Meyer R., Van Craynest M. P., Maldague P. Pancreatic glucagon does not alter mucosal growth and maturation of sucrase and thymidine kinase activity in rat small intestine. Biol Neonate. 1983;43(5-6):253–262. doi: 10.1159/000241652. [DOI] [PubMed] [Google Scholar]
  4. Conteas C. N., Majumdar A. P. The effects of gastrin, epidermal growth factor, and somatostatin on DNA synthesis in a small intestinal crypt cell line (IEC-6). Proc Soc Exp Biol Med. 1987 Mar;184(3):307–311. doi: 10.3181/00379727-184-42484. [DOI] [PubMed] [Google Scholar]
  5. Gornacz G. E., Ghatei M. A., Al-Mukhtar M. Y., Yeats J. C., Adrian T. E., Wright N. A., Bloom S. R. Plasma enteroglucagon and CCK levels and cell proliferation in defunctioned small bowel in the rat. Dig Dis Sci. 1984 Nov;29(11):1041–1049. doi: 10.1007/BF01311257. [DOI] [PubMed] [Google Scholar]
  6. Gregory R. A., Grossman M. I., Tracy H. J., Bentley P. H. Nature of the gastric secretagogue in Zollinger-Ellison tumours. Lancet. 1967 Sep 9;2(7515):543–544. doi: 10.1016/s0140-6736(67)90501-6. [DOI] [PubMed] [Google Scholar]
  7. Holst J. J. Gut glucagon, enteroglucagon, gut glucagonlike immunoreactivity, glicentin--current status. Gastroenterology. 1983 Jun;84(6):1602–1613. [PubMed] [Google Scholar]
  8. Johnson L. R. Effect of exogenous gut hormones on gastrointestinal mucosal growth. Scand J Gastroenterol Suppl. 1982;74:89–92. [PubMed] [Google Scholar]
  9. Johnson L. R., Guthrie P. D. Secretin inhibition of gastrin-stimulated deoxyribonucleic acid synthesis. Gastroenterology. 1974 Oct;67(4):601–606. [PubMed] [Google Scholar]
  10. Johnson L. R., Guthrie P. D. Stimulation of DNA synthesis by big and little gastrin (G-34 and G-17). Gastroenterology. 1976 Oct;71(4):599–602. [PubMed] [Google Scholar]
  11. Johnson L. R., Guthrie P. Effect of cholecystokinin and 16,16-dimethyl prostaglandin E2 on RNA and DNA of gastric and duodenal mucosa. Gastroenterology. 1976 Jan;70(1):59–65. [PubMed] [Google Scholar]
  12. Lehy T., Puccio F., Chariot J., Labeille D. Stimulating effect of bombesin on the growth of gastrointestinal tract and pancreas in suckling rats. Gastroenterology. 1986 Jun;90(6):1942–1949. doi: 10.1016/0016-5085(86)90265-9. [DOI] [PubMed] [Google Scholar]
  13. Lucas A., Adrian T. E., Christofides N., Bloom S. R., Aynsley-Green A. Plasma motilin, gastrin, and enteroglucagon and feeding in the human newborn. Arch Dis Child. 1980 Sep;55(9):673–677. doi: 10.1136/adc.55.9.673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lucas A., Bloom S. R., Aynsley-Green A. Gut hormones and 'minimal enteral feeding'. Acta Paediatr Scand. 1986 Sep;75(5):719–723. doi: 10.1111/j.1651-2227.1986.tb10280.x. [DOI] [PubMed] [Google Scholar]
  15. Lucas A., Bloom S. R., Aynsley-Green A. Metabolic and endocrine consequences of depriving preterm infants of enteral nutrition. Acta Paediatr Scand. 1983 Mar;72(2):245–249. doi: 10.1111/j.1651-2227.1983.tb09705.x. [DOI] [PubMed] [Google Scholar]
  16. Lucas A., Bloom S. R., Aynsley-Green A. Postnatal surges in plasma gut hormones in term and preterm infants. Biol Neonate. 1982;41(1-2):63–67. doi: 10.1159/000241518. [DOI] [PubMed] [Google Scholar]
  17. Morisset J., Génik P. Effects of acute and chronic administration of secretin and caerulein on rat duodenal and gastric growth. Regul Pept. 1983 Jan;5(2):111–123. doi: 10.1016/0167-0115(83)90119-2. [DOI] [PubMed] [Google Scholar]
  18. Moyer M. P., Armstrong A., Aust J. B., Levine B. A., Sirinek K. R. Effects of gastrin, glutamine, and somatostatin on the in vitro growth of normal and malignant human gastric mucosal cells. Arch Surg. 1986 Mar;121(3):285–288. doi: 10.1001/archsurg.1986.01400030039006. [DOI] [PubMed] [Google Scholar]
  19. Moyer M. P., Aust J. B., Dixon P. S., Levine B. A., Sirinek K. R. Glucagon enhances growth of cultured human colorectal cancer cells in vitro. Am J Surg. 1985 Dec;150(6):676–679. doi: 10.1016/0002-9610(85)90406-4. [DOI] [PubMed] [Google Scholar]
  20. Pansu D., Berard A., Dechelette M. A., Lambert R. Influence of scretin and pentagastrin on the circadian rhythm of cell proliferation in the intestinal mucosa in rats. Digestion. 1974;11(3-4):266–274. doi: 10.1159/000197591. [DOI] [PubMed] [Google Scholar]
  21. Sirinek K. R., Levine B. A., Moyer M. P. Pentagastrin stimulates in vitro growth of normal and malignant human colon epithelial cells. Am J Surg. 1985 Jan;149(1):35–39. doi: 10.1016/s0002-9610(85)80006-4. [DOI] [PubMed] [Google Scholar]
  22. Stange E. F., Schneider A., Seiffer E., Ditschuneit H. Effect of pentagastrin, secretin and cholecystokinin on growth and differentiation in organ cultured rabbit small intestine. Horm Metab Res. 1986 May;18(5):303–307. doi: 10.1055/s-2007-1012302. [DOI] [PubMed] [Google Scholar]
  23. Stanley M. D., Coalson R. E., Grossman M. I., Johnson L. R. Influence of secretin and pentagastrin on acid secretion and parietal cell number in rats. Gastroenterology. 1972 Aug;63(2):264–269. [PubMed] [Google Scholar]

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