Skip to main content
NCBI home page
Gut logoLink to Gut
. 1987;28(Suppl):197–201. doi: 10.1136/gut.28.suppl.197

Effect of experimental pancreatic growth on the content of xenobiotic-metabolising enzymes in the pancreas.

J Ross 1, J A Barrowman 1
PMCID: PMC1434561  PMID: 3121455

Abstract

The concentration in pancreatic tissue and the total pancreatic content of three xenobiotic metabolising enzymes has been determined in two models of experimental pancreatic growth namely, cholecystokinin-octapeptide injections and soy flour feeding. No significant change in pancreatic concentrations of benzo(a)pyrene hydroxylase or glucuronyl transferase was detected. In both models of pancreatic growth, however, the concentration of glutathione-S-transferase was significantly reduced. It is possible that the reduction in this enzyme may be of some importance in determining the susceptibility of the pancreas to carcinogenesis observed with long term soy flour feeding.

Full text

PDF
197

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benson A. M., Cha Y. N., Bueding E., Heine H. S., Talalay P. Elevation of extrahepatic glutathione S-transferase and epoxide hydratase activities by 2(3)-tert-butyl-4-hydroxyanisole. Cancer Res. 1979 Aug;39(8):2971–2977. [PubMed] [Google Scholar]
  2. Black O., Jr, Howerton B. K. Glutathione S-transferase activity in rat pancreas. J Natl Cancer Inst. 1984 Jan;72(1):121–123. doi: 10.1093/jnci/72.1.121. [DOI] [PubMed] [Google Scholar]
  3. Dallner G. Isolation of rough and smooth microsomes--general. Methods Enzymol. 1974;31:191–201. [PubMed] [Google Scholar]
  4. Green G. M., Lyman R. L. Feedback regulation of pancreatic enzyme secretion as a mechanism for trypsin inhibitor-induced hypersecretion in rats. Proc Soc Exp Biol Med. 1972 May;140(1):6–12. doi: 10.3181/00379727-140-36384. [DOI] [PubMed] [Google Scholar]
  5. Habig W. H., Pabst M. J., Jakoby W. B. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem. 1974 Nov 25;249(22):7130–7139. [PubMed] [Google Scholar]
  6. Hoensch H. P., Steinhardt H. J., Weiss G., Haug D., Maier A., Malchow H. Effects of semisynthetic diets on xenobiotic metabolizing enzyme activity and morphology of small intestinal mucosa in humans. Gastroenterology. 1984 Jun;86(6):1519–1530. [PubMed] [Google Scholar]
  7. Howatson A. G., Carter D. C. Pancreatic carcinogenesis-enhancement by cholecystokinin in the hamster-nitrosamine model. Br J Cancer. 1985 Jan;51(1):107–114. doi: 10.1038/bjc.1985.15. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kandaswami C., O'Brien P. J. Effect of chrysotile asbestos and silica on the microsomal metabolism of benzo(a)pyrene. Environ Health Perspect. 1983 Sep;51:311–314. doi: 10.1289/ehp.8351311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Liddle R. A., Goldfine I. D., Williams J. A. Bioassay of plasma cholecystokinin in rats: effects of food, trypsin inhibitor, and alcohol. Gastroenterology. 1984 Sep;87(3):542–549. [PubMed] [Google Scholar]
  10. Lin R. S., Kessler I. I. A multifactorial model for pancreatic cancer in man. Epidemiologic evidence. JAMA. 1981 Jan 9;245(2):147–152. [PubMed] [Google Scholar]
  11. Longnecker D. S., Curphey T. J. Adenocarcinoma of the pancreas in azaserine-treated rats. Cancer Res. 1975 Aug;35(8):2249–2258. [PubMed] [Google Scholar]
  12. Longnecker D. S., Wiebkin P., Schaeffer B. K., Roebuck B. D. Experimental carcinogenesis in the pancreas. Int Rev Exp Pathol. 1984;26:177–229. [PubMed] [Google Scholar]
  13. McGuinness E. E., Morgan R. G., Wormsley K. G. Effects of soybean flour on the pancreas of rats. Environ Health Perspect. 1984 Jun;56:205–212. doi: 10.1289/ehp.8456205. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Morgan R. G., Levinson D. A., Hopwood D., Saunders J. H., Wormsley K. G. Potentiation of the action of azaserine on the rat pancreas by raw soya bean flour. Cancer Lett. 1977 Jul;3(1-2):87–90. doi: 10.1016/s0304-3835(77)94455-x. [DOI] [PubMed] [Google Scholar]
  15. Sparnins V. L., Venegas P. L., Wattenberg L. W. Glutathione S-transferase activity: enhancement by compounds inhibiting chemical carcinogenesis and by dietary constituents. J Natl Cancer Inst. 1982 Mar;68(3):493–496. [PubMed] [Google Scholar]
  16. Tartakoff A. M., Jamieson J. D. Subcellular fractionation of the pancreas. Methods Enzymol. 1974;31:41–59. doi: 10.1016/0076-6879(74)31006-3. [DOI] [PubMed] [Google Scholar]
  17. Temler R. S., Dormond C. A., Simon E., Morel B. The effect of feeding soybean trypsin inhibitor and repeated injections of cholecystokinin on rat pancreas. J Nutr. 1984 Jun;114(6):1083–1091. doi: 10.1093/jn/114.6.1083. [DOI] [PubMed] [Google Scholar]
  18. Van Cantfort J., De Graeve J., Gielen J. E. Radioactive assay for aryl hydrocarbon hydroxylase. Improved method and biological importance. Biochem Biophys Res Commun. 1977 Nov 21;79(2):505–512. doi: 10.1016/0006-291x(77)90186-3. [DOI] [PubMed] [Google Scholar]

Articles from Gut are provided here courtesy of BMJ Publishing Group

RESOURCES