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. 1987;28(Suppl):203–205. doi: 10.1136/gut.28.suppl.203

Tryptophan rich diet as a new approach to study the serotoninergic enteropancreatic axis.

B Goke 1, G Richter 1, V Keim 1, R Arnold 1
PMCID: PMC1434567  PMID: 3692309

Abstract

The influence of a tryptophan enriched diet (L-tryptophan added as 1% of total diet), fed over 10 days, on the rat duodenum and pancreas was studied by immunohistology, measurements of serotonin and tryptophan tissue concentrations by HPLC, and incubations of pancreatic lobules. Ingestion of a tryptophan enriched diet resulted in increased contents of tryptophan and serotonin in the duodenum that was not accompanied by a significant change of the serotonin cell density. Neither basal nor CCK-stimulated amylase release from isolated pancreatic lobules was altered after tryptophan enriched food. Although serotonin could be extracted from the pancreas, no increase in serotonin concentration was detected after ingestion of the tryptophan diet. A 'serotonin loading' diet may be a useful tool to study the significance of amines produced by gut endocrine cells in respects to enteropancreatic connections.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnold R., Hülst M. V., Neuhof C. H., Schwarting H., Becker H. D., Creutzfeldt W. Antral gastrin-producing G-cells and somatostatin-producing D-cells in different states of gastric acid secretion. Gut. 1982 Apr;23(4):285–291. doi: 10.1136/gut.23.4.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Falck B., Owman C. 5-hydroxytryptamine and related amines in endocrine cell systems. Adv Pharmacol. 1968;6(Pt A):211–231. doi: 10.1016/s1054-3589(08)61175-8. [DOI] [PubMed] [Google Scholar]
  3. Gylfe E., Hellman B., Sehlin J., Taljedal I. B. Amino acid conversion into 5-hydroxytryptamine in pancreatic beta-cells. Endocrinology. 1973 Oct;93(4):932–937. doi: 10.1210/endo-93-4-932. [DOI] [PubMed] [Google Scholar]
  4. Göke B., Printz H., Koop I., Rausch U., Richter G., Arnold R., Adler G. Endogenous CCK release and pancreatic growth in rats after feeding a proteinase inhibitor (camostate). Pancreas. 1986;1(6):509–515. doi: 10.1097/00006676-198611000-00008. [DOI] [PubMed] [Google Scholar]
  5. Inokuchi H., Kawai K., Takeuchi Y., Sano Y. Immunohistochemical demonstration of EC cells in rat gastrointestinal tract. Histochemistry. 1982;74(4):453–456. doi: 10.1007/BF00496658. [DOI] [PubMed] [Google Scholar]
  6. Inokuchi H., Kawai K., Takeuchi Y., Sano Y. Immunohistochemical study on the morphology of enterochromaffin cells in the human fundic mucosa. Cell Tissue Res. 1984;235(3):703–705. doi: 10.1007/BF00226973. [DOI] [PubMed] [Google Scholar]
  7. Larsson L. I. Gastrointestinal cells producing endocrine, neurocrine and paracrine messengers. Clin Gastroenterol. 1980 Sep;9(3):485–416. [PubMed] [Google Scholar]
  8. Richter G., Stöckmann F., Conlon J. M., Creutzfeldt W. Serotonin release into blood after food and pentagastrin. Studies in healthy subjects and in patients with metastatic carcinoid tumors. Gastroenterology. 1986 Sep;91(3):612–618. doi: 10.1016/0016-5085(86)90630-x. [DOI] [PubMed] [Google Scholar]
  9. Scheele G. A., Palade G. E. Studies on the guinea pig pancreas. Parallel discharge of exocrine enzyme activities. J Biol Chem. 1975 Apr 10;250(7):2660–2670. [PubMed] [Google Scholar]
  10. Stern L., Tenenhouse A., Yu E. W. Uptake, storage and secretion of 5-hydroxytryptamine and its amino acid precursor by dispersed rat pancreas acinar cells. J Physiol. 1983 Jul;340:555–567. doi: 10.1113/jphysiol.1983.sp014780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Thompson J. H. Serotonin and the alimentary tract. Res Commun Chem Pathol Pharmacol. 1971 Jul-Sep;2(4):687–781. [PubMed] [Google Scholar]

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