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. 1983 Oct;44(1):49–57. doi: 10.1016/S0006-3495(83)84276-3

Electrogenic H+/OH- movement across phospholipid vesicles measured by spin-labeled hydrophobic ions.

D S Cafiso, W L Hubbell
PMCID: PMC1434809  PMID: 6313085

Abstract

Transmembrane pH gradients created across phospholipid vesicles give rise to time-dependent potentials as determined from the EPR spectra of phosphonium ion spin labels in the system. From the time-dependent spectra, the transmembrane H+/OH- current is obtained and hence the current-voltage curve for the vesicle membrane is obtained. The current-voltage curve is linear with a membrane resistance of 3 +/- 2 X 10(9) omega cm2 corresponding to a membrane permeability of 5 +/- 2 X 10(-7) cm/s. This unusually high permeability is further increased by small amounts of lipid oxidation, CHCl3 or the general anesthetic halothane.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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