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. 1983 Dec;44(3):315–324. doi: 10.1016/S0006-3495(83)84305-7

A continuously variable frequency cross-correlation phase fluorometer with picosecond resolution.

E Gratton, M Limkeman
PMCID: PMC1434839  PMID: 6661490

Abstract

A detailed description of the construction and performance of a variable frequency cross-correlation phase fluorometer is reported. The phase fluorometer operates over the frequency range 1-160 MHz with a maximum resolution of a few picoseconds. The effects of distortions introduced by the light modulator and the nonlinear dynode characteristic are discussed in terms of the harmonic content of the detected signal. A source of systematic errors due to nonhomogeneous modulation is also discussed with particular attention to the color effect of the photomultipliers. The application of the phase fluorometer to the measurement of very long and very short lifetimes is reported. Some application to the measurement of multiexponential decays is also illustrated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Lakowicz J. R., Cherek H., Balter A. Correction of timing errors in photomultiplier tubes used in phase-modulation fluorometry. J Biochem Biophys Methods. 1981 Sep;5(3):131–146. doi: 10.1016/0165-022x(81)90012-9. [DOI] [PubMed] [Google Scholar]

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