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. 1984 Jan;45(1):208–218. doi: 10.1016/S0006-3495(84)84149-1

Gap junction structures. VI. Variation and conservation in connexon conformation and packing.

L Makowski, D L Caspar, W C Phillips, T S Baker, D A Goodenough
PMCID: PMC1435272  PMID: 6324904

Abstract

Correlation of structural changes in isolated gap junctions with the mechanism of channel gating is complicated by the effects of isolation procedures and the lack of a direct functional assay. The effect of variations in the isolation procedure are examined by comparison of the structures of gap junctions isolated by different protocols. X-ray diffraction data from over two hundren specimens are compared to provide a basis for identification of invariant aspects of the connexon structure and variable properties related either to functional switching or experimental modifications. We discuss the relationship between subunit tilt, lattice symmetry and packing, and membrane curvature and demonstrate that membrane curvature may be a natural consequence of the structure of the connexons and the patterns of interactions between them.

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212FIGURE 4
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214FIGURE 6
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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker T. S., Caspar D. L., Hollingshead C. J., Goodenough D. A. Gap junction structures. IV. Asymmetric features revealed by low-irradiation microscopy. J Cell Biol. 1983 Jan;96(1):204–216. doi: 10.1083/jcb.96.1.204. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baldwin K. M. Cardiac gap junction configuration after an uncoupling treatment as a function of time. J Cell Biol. 1979 Jul;82(1):66–75. doi: 10.1083/jcb.82.1.66. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baldwin K. M. The fine structure of healing over in mammalian cardiac muscle. J Mol Cell Cardiol. 1977 Dec;9(12):959–966. doi: 10.1016/s0022-2828(77)80049-7. [DOI] [PubMed] [Google Scholar]
  4. Bennett M. V. Function of electrotonic junctions in embryonic and adult tissues. Fed Proc. 1973 Jan;32(1):65–75. [PubMed] [Google Scholar]
  5. Bennett M. V., Goodenough D. A. Gap junctions, electrotonic coupling, and intercellular communication. Neurosci Res Program Bull. 1978 Sep;16(3):1–486. [PubMed] [Google Scholar]
  6. Caspar D. L., Goodenough D. A., Makowski L., Phillips W. C. Gap junction structures. I. Correlated electron microscopy and x-ray diffraction. J Cell Biol. 1977 Aug;74(2):605–628. doi: 10.1083/jcb.74.2.605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fallon R. F., Goodenough D. A. Five-hour half-life of mouse liver gap-junction protein. J Cell Biol. 1981 Aug;90(2):521–526. doi: 10.1083/jcb.90.2.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Goodenough D. A. Bulk isolation of mouse hepatocyte gap junctions. Characterization of the principal protein, connexin. J Cell Biol. 1974 May;61(2):557–563. doi: 10.1083/jcb.61.2.557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Goodenough D. A. In vitro formation of gap junction vesicles. J Cell Biol. 1976 Feb;68(2):220–231. doi: 10.1083/jcb.68.2.220. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Goodenough D. A., Revel J. P. A fine structural analysis of intercellular junctions in the mouse liver. J Cell Biol. 1970 May;45(2):272–290. doi: 10.1083/jcb.45.2.272. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Henderson D., Eibl H., Weber K. Structure and biochemistry of mouse hepatic gap junctions. J Mol Biol. 1979 Aug 5;132(2):193–218. doi: 10.1016/0022-2836(79)90391-7. [DOI] [PubMed] [Google Scholar]
  12. Hertzberg E. L., Gilula N. B. Isolation and characterization of gap junctions from rat liver. J Biol Chem. 1979 Mar 25;254(6):2138–2147. [PubMed] [Google Scholar]
  13. Kirschner D. A., Caspar D. L. Comparative diffraction studies on myelin membranes. Ann N Y Acad Sci. 1972 Jun 20;195:309–320. [PubMed] [Google Scholar]
  14. Makowski L., Caspar D. L., Goodenough D. A., Phillips W. C. Gap Junction Structures: III. The Effect of Variations in the Isolation Procedure. Biophys J. 1982 Jan;37(1):189–191. doi: 10.1016/S0006-3495(82)84663-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Makowski L., Caspar D. L., Phillips W. C., Goodenough D. A. Gap junction structures. II. Analysis of the x-ray diffraction data. J Cell Biol. 1977 Aug;74(2):629–645. doi: 10.1083/jcb.74.2.629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nicholson B. J., Hunkapiller M. W., Grim L. B., Hood L. E., Revel J. P. Rat liver gap junction protein: properties and partial sequence. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7594–7598. doi: 10.1073/pnas.78.12.7594. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Peracchia C. Gap junctions. Structural changes after uncoupling procedures. J Cell Biol. 1977 Mar;72(3):628–641. doi: 10.1083/jcb.72.3.628. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Raviola E., Goodenough D. A., Raviola G. Structure of rapidly frozen gap junctions. J Cell Biol. 1980 Oct;87(1):273–279. doi: 10.1083/jcb.87.1.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Raviola G., Raviola E. Intercellular junctions in the ciliary epithelium. Invest Ophthalmol Vis Sci. 1978 Oct;17(10):958–981. [PubMed] [Google Scholar]
  20. Unwin P. N., Zampighi G. Structure of the junction between communicating cells. Nature. 1980 Feb 7;283(5747):545–549. doi: 10.1038/283545a0. [DOI] [PubMed] [Google Scholar]
  21. Zampighi G., Unwin P. N. Two forms of isolated gap junctions. J Mol Biol. 1979 Dec 5;135(2):451–464. doi: 10.1016/0022-2836(79)90446-7. [DOI] [PubMed] [Google Scholar]

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