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. 1984 Feb 4;288(6414):349–352. doi: 10.1136/bmj.288.6414.349

Autoantibodies against human insulin.

T J Wilkin, S Nicholson
PMCID: PMC1444218  PMID: 6419924

Abstract

Sera from 680 non-diabetic subjects with suspected autoimmune disease were screened for 13 different antibodies. Of the 582 sera found to contain these antibodies, nine bound insulin in an IgG specific enzyme linked immunosorbent assay (micro ELISA). Four of the sera bound human, porcine, and bovine insulins and five bound exclusively human insulin. "Cold" human, porcine, and bovine insulins each displaced, in a dose dependent manner, the four sera which bound all three insulins, but only human insulin displaced the remaining five, porcine and bovine insulins having little or no effect in concentrations up to 1000 U/1. These observations point to the existence of autoantibodies specifically against human insulin in some subjects with established autoimmunity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bottazzo G. F., Florin-Christensen A., Doniach D. Islet-cell antibodies in diabetes mellitus with autoimmune polyendocrine deficiencies. Lancet. 1974 Nov 30;2(7892):1279–1283. doi: 10.1016/s0140-6736(74)90140-8. [DOI] [PubMed] [Google Scholar]
  2. Cahill G. F., Jr, McDevitt H. O. Insulin-dependent diabetes mellitus: the initial lesion. N Engl J Med. 1981 Jun 11;304(24):1454–1465. doi: 10.1056/NEJM198106113042403. [DOI] [PubMed] [Google Scholar]
  3. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
  4. Ewins D. L., Wilkin T. J. A clinical comparison of the enzyme-linked immunosorbent assay (ELISA) and haemagglutination (TRC) in the routine detection of antithyroglobulin antibodies. Acta Endocrinol (Copenh) 1983 Jun;103(2):216–222. doi: 10.1530/acta.0.1030216. [DOI] [PubMed] [Google Scholar]
  5. Folling I., Norman N. Hyperglycemia, hypoglycemic attacks, and production of anti-insulin antibodies without previous known immunization. Immunological and functional studies in a patient. Diabetes. 1972 Jul;21(7):814–826. doi: 10.2337/diab.21.7.814. [DOI] [PubMed] [Google Scholar]
  6. Goldman J., Baldwin D., Rubenstein A. H., Klink D. D., Blackard W. G., Fisher L. K., Roe T. F., Schnure J. J. Characterization of circulating insulin and proinsulin-binding antibodies in autoimmune hypoglycemia. J Clin Invest. 1979 May;63(5):1050–1059. doi: 10.1172/JCI109374. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hirata Y., Ishizu H. Elevated insulin-binding capacity of serum proteins in a case with spontaneous hypoglycemia and mild diabetes not treated with insulin. Tohoku J Exp Med. 1972 Jul;107(3):277–286. doi: 10.1620/tjem.107.277. [DOI] [PubMed] [Google Scholar]
  8. Kuzuya T., Matsuda A., Saito T., Yoshida S. Problems on the specificity of iodinated insulin binding by sera of patients with autoimmune thyroid disorders. Endocrinol Jpn. 1978 Dec;25(6):597–606. doi: 10.1507/endocrj1954.25.597. [DOI] [PubMed] [Google Scholar]
  9. Oneda A., Matsuda K., Sato M., Yamagata S., Sato T. Hypoglycemia due to apparent autoantibodies to insulin. Characterization of insulin-binding protein. Diabetes. 1974 Jan;23(1):41–50. doi: 10.2337/diab.23.1.41. [DOI] [PubMed] [Google Scholar]
  10. Onodera T., Ray U. R., Melez K. A., Suzuki H., Toniolo A., Notkins A. L. Virus-induced diabetes mellitus: autoimmunity and polyendocrine disease prevented by immunosuppression. Nature. 1982 May 6;297(5861):66–68. doi: 10.1038/297066a0. [DOI] [PubMed] [Google Scholar]
  11. Onodera T., Toniolo A., Ray U. R., Jenson A. B., Knazek R. A., Notkins A. L. Virus-induced diabetes mellitus. XX. Polyendocrinopathy and autoimmunity. J Exp Med. 1981 Jun 1;153(6):1457–1473. doi: 10.1084/jem.153.6.1457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Reeves W. G., Kelly U. An immunochemical method for the quantitation of insulin antibodies. J Immunol Methods. 1980;34(4):329–338. doi: 10.1016/0022-1759(80)90105-2. [DOI] [PubMed] [Google Scholar]
  13. Solomon G., Schiffenbauer J., Keiser H. D., Diamond B. Use of monoclonal antibodies to identify shared idiotypes on human antibodies to native DNA from patients with systemic lupus erythematosus. Proc Natl Acad Sci U S A. 1983 Feb;80(3):850–854. doi: 10.1073/pnas.80.3.850. [DOI] [PMC free article] [PubMed] [Google Scholar]

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