Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1976 Jan;30(1):145–155.

Evidence against T-cell immunoglobulin from radioimmunoassay on serum and cells from bursectomized chickens.

J C Jensenius
PMCID: PMC1444950  PMID: 1082438

Abstract

A radioimmunoassay with a sensitivity of about 50 pg of chicken immunoglobulin (Ig) was developed and employed for the estimation of Ig in the serum and in extracts of cells from hormonally bursectomized chickens. Chickens, which were judged agammaglobulinaemic by rocket immunoelectrophoresis, had at 12 weeks of age 5-100 ng of Ig/ml of serum. The Ig concentration was decreasing with a half life of 5 days. Thymus and spleen cells from such hypogammaglobulinaemic chickens were extracted with non-ionic detergents, acid urea, or combinations of urea and detergent, and the extracts were analysed for Ig by the inhibition assay. The amount of Ig found in extracts from thymocytes corresponded to less than a hundred molecules of 7S Ig/cell with a lower value of less than five molecules/cell, while extracts of spleen cells contained Ig corresponding to 60-300 molecules of Ig/cell. The inclusion in the media of a number of protease inhibitors did not increase the recovery. Extracts of thymus and spleen cells from untreated chickens contained Ig corresponding to 10-20 X 10(3) and 50-150 X 10(3) molecules of Ig/cell respectively. The results demonstrate the profound hypogammaglobulinaemia of bursectomized chickens and question the concept of an active production of receptor Ig by bursa-independent T cells.

Full text

PDF
145

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersson J., Lafleur L., Melchers IgM in bone marrow-derived lymphocytes. Synthesis, surface deposition, turnover and carbohydrate composition in unstimulated mouse B cells. Eur J Immunol. 1974 Mar;4(3):170–180. doi: 10.1002/eji.1830040305. [DOI] [PubMed] [Google Scholar]
  2. Basten A., Miller J. F., Warner N. L., Pye J. Specific inactivation of thymus-derived (T) and non-thymus-derived (B) lymphocytes by 125I-labelled antigen. Nat New Biol. 1971 May 26;231(21):104–106. doi: 10.1038/newbio231104a0. [DOI] [PubMed] [Google Scholar]
  3. Bowers W. E., Finkenstaedt J. T., de Duve C. Lysosomes in lymphoid tissue. I. The measurement of hydrolytic activities in whole homogenates. J Cell Biol. 1967 Feb;32(2):325–337. doi: 10.1083/jcb.32.2.325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bowers W. E. Lysosomes in rat thoracic duct lymphocytes. J Exp Med. 1972 Dec 1;136(6):1394–1403. doi: 10.1084/jem.136.6.1394. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Crone M., Koch C., Simonsen M. The elusive T cell receptor. Transplant Rev. 1972;10:36–56. doi: 10.1111/j.1600-065x.1972.tb01538.x. [DOI] [PubMed] [Google Scholar]
  6. Fairbanks G., Steck T. L., Wallach D. F. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. doi: 10.1021/bi00789a030. [DOI] [PubMed] [Google Scholar]
  7. Frommel D., Perey D. Y., Good R. A. Metabolism of gamma G and gamma M immunoglobulins in normal and hypogammaglobulinemic chickens. J Immunol. 1970 Jul;105(1):1–6. [PubMed] [Google Scholar]
  8. Grey H. M., Kubo R. T., Cerottini J. C. Thymus-derived (T) cell immunoglobulins. Presence of a receptor site for IgG and absence of large amounts of "buried" Ig determinants on T cells. J Exp Med. 1972 Nov 1;136(5):1323–1328. doi: 10.1084/jem.136.5.1323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hudson L., Thantrey N., Roitt I. M. The bursa dependence of chicken thymus-derived lymphocyte surface immunoglobulin. Immunology. 1975 Jan;28(1):151–159. [PMC free article] [PubMed] [Google Scholar]
  10. Hunt S. V., Williams A. F. The origin of cell surface immunoglobulin of marrow-derived and thymus-derived lymphocytes of the rat. J Exp Med. 1974 Mar 1;139(3):479–496. doi: 10.1084/jem.139.3.479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hämmerling G. J., McDevitt H. O. Antigen binding T and B lymphocytes. I. Differences in cellular specificity and influence of metabolic activity on interaction of antigen with T and B cells. J Immunol. 1974 May;112(5):1726–1733. [PubMed] [Google Scholar]
  12. Jensenius C., Crone M., Koch C. Antigen binding by non-bursa-derived chicken leukocytes. Scand J Immunol. 1975;4(2):151–160. doi: 10.1111/j.1365-3083.1975.tb02612.x. [DOI] [PubMed] [Google Scholar]
  13. Jensenius J. C., Williams A. F. Total immunoglobulin of rat thymocytes and thoracic duct lymphocytes. Eur J Immunol. 1974 Feb;4(2):98–105. doi: 10.1002/eji.1830040208. [DOI] [PubMed] [Google Scholar]
  14. Kincade P. W., Lawton A. R., Bockman D. E., Cooper M. D. Suppression of immunoglobulin G synthesis as a result of antibody-mediated suppression of immunoglobulin M synthesis in chickens. Proc Natl Acad Sci U S A. 1970 Dec;67(4):1918–1925. doi: 10.1073/pnas.67.4.1918. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kincade P. W., Lawton A. R., Cooper M. D. Restriction of surface immunoglobulin determinants to lymphocytes of the plasma cell line. J Immunol. 1971 May;106(5):1421–1423. [PubMed] [Google Scholar]
  16. Laskov R., Scharff M. D. Independent synthesis of light and heavy chains: quantitation of light-chain production by mouse myeloma variants. J Exp Med. 1974 Oct 1;140(4):1112–1116. doi: 10.1084/jem.140.4.1112. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lebacq-Verheyden A. M., Vaerman J. P., Heremans J. F. Quantification and distribution of chicken immunoglobulins IgA, IgM and IgG in serum and secretions. Immunology. 1974 Oct;27(4):683–692. [PMC free article] [PubMed] [Google Scholar]
  18. Leslie G. A., Clem L. W. Phylogen of immunoglobulin structure and function. 3. Immunoglobulins of the chicken. J Exp Med. 1969 Dec 1;130(6):1337–1352. doi: 10.1084/jem.130.6.1337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Leslie G. A., Martin L. N. Studies on the secretory immunologic system of fowl. 3. Serum and secretory IgA of the chicken. J Immunol. 1973 Jan;110(1):1–9. [PubMed] [Google Scholar]
  20. Lisowska-Bernstein B., Rinuy A., Vassalli P. Absence of detectable IgM in enzymatically or biosynthetically labeled thymus-derived lymphocytes. Proc Natl Acad Sci U S A. 1973 Oct;70(10):2879–2883. doi: 10.1073/pnas.70.10.2879. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Mann D. L. The effect of enzyme inhibitors on the solubilization of HL-A antigens with 3 M KCl. Transplantation. 1972 Sep;14(3):398–401. [PubMed] [Google Scholar]
  22. Marchalonis J. J., Cone R. E., Atwell J. L. Isolation and partial characterization of lymphocyte surface immunoglobulins. J Exp Med. 1972 Apr 1;135(4):956–971. doi: 10.1084/jem.135.4.956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Moroz C., Hahn Y. Cell-surface immunoglobulin human thymus cells and its biosynthesis in vitro. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3716–3720. doi: 10.1073/pnas.70.12.3716. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Muirhead M. L., Action R. T., Williams A. F. Preliminary characterization of Thy-1.1 and Ag-B antigens from rat tissues solubilized in detergents. Biochem J. 1974 Oct;143(1):51–61. doi: 10.1042/bj1430051. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. ROWE D. S., FAHEY J. L. A NEW CLASS OF HUMAN IMMUNOGLOBULINS. II. NORMAL SERUM IGD. J Exp Med. 1965 Jan 1;121:185–199. doi: 10.1084/jem.121.1.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Ramseier H. Spontaneous release of T-cell receptors for alloantigens. I. Recognition of alloantigens and receptor release dynamics. J Exp Med. 1974 Sep 1;140(3):603–618. doi: 10.1084/jem.140.3.603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Roelants G. Antigen recognition by B and T lymphocytes. Curr Top Microbiol Immunol. 1972;59:135–165. doi: 10.1007/978-3-642-65444-2_5. [DOI] [PubMed] [Google Scholar]
  28. Roelants G., Rydén A. Dose dependence of antigen binding to B and T lymphocytes. Nature. 1974 Jan 11;247(5436):104–106. doi: 10.1038/247104a0. [DOI] [PubMed] [Google Scholar]
  29. Rowe D. S., Hug K., Forni L., Pernis B. Immunoglobulin D as a lymphocyte receptor. J Exp Med. 1973 Oct 1;138(4):965–972. doi: 10.1084/jem.138.4.965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Rubin B., Wigzell H. The immune response against hapten-autologous protein conjugates in the mouse. J Exp Med. 1973 Apr 1;137(4):911–931. doi: 10.1084/jem.137.4.911. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Santer V., Cone R. E., Marchalonis J. J. The glycoprotein surface coat on different classes of murine lymphocytes. Exp Cell Res. 1973 Jun;79(2):404–416. doi: 10.1016/0014-4827(73)90460-6. [DOI] [PubMed] [Google Scholar]
  32. Solomon A., McLaughlin C. L. Biosynthesis of low molecular weight (7S) and high molecular weight (19S) immunoglobulin M. J Clin Invest. 1970 Jan;49(1):150–160. doi: 10.1172/JCI106214. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Spiegelberg H. L., Prahl J. W., Grey H. M. Structural studies of human gamma D myeloma protein. Biochemistry. 1970 May 12;9(10):2115–2122. doi: 10.1021/bi00812a013. [DOI] [PubMed] [Google Scholar]
  34. Szenberg A., Cone R. E., Marchalonis J. J. Isolation of surface immunoglobulins from lymphocytes from chicken thymus and bursa. Nature. 1974 Aug 2;250(465):418–420. doi: 10.1038/250418a0. [DOI] [PubMed] [Google Scholar]
  35. Vitetta E. S., Bianco C., Nussenzweig V., Uhr J. W. Cell surface immunoglobulin. IV. Distribution among thymocytes, bone mrrow cells, and their derived populations. J Exp Med. 1972 Jul 1;136(1):81–93. doi: 10.1084/jem.136.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. WARNER N. L., SZENBERG A., BURNET F. M. The immunological role of different lymphoid organs in the chicken. I. Dissociation of immunological responsiveness. Aust J Exp Biol Med Sci. 1962 Oct;40:373–387. doi: 10.1038/icb.1962.42. [DOI] [PubMed] [Google Scholar]
  37. Warner N. L., Ovary Z., Kantor F. S. Delayed hypersensitivity reactions in normal and bursectomized chickens. Int Arch Allergy Appl Immunol. 1971;40(4-5):719–728. doi: 10.1159/000230454. [DOI] [PubMed] [Google Scholar]
  38. Warner N. L., Uhr J. W., Thorbecke G. J., Ovary Z. Immunoglobulins, antibodies and the bursa of Fabricus: induction of agammaglobulinemia and the loss of all antibody-forming capacity by hormonal bursectomy. J Immunol. 1969 Dec;103(6):1317–1330. [PubMed] [Google Scholar]
  39. Weinbaum F. I., Gilmour D. G., Thorbecke G. J. Immunocompetent cells of the chicken. 3. Cooperation of carrier sensitized T cells from agammaglobulinemic donors with hapten immune B cells. J Immunol. 1973 May;110(5):1434–1436. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES