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. 1976 Feb;30(2):171–179.

Incompatibility between C3b and B of guinea-pig and man and its influence on the titration of the alternative pathway factors D and B in these two species.

V Brade, L Dieminger, G Schmidt, W Vogt
PMCID: PMC1444978  PMID: 57093

Abstract

The incubation of the proteins C3b, B, D and C3 induces enzymatic activation of B and of C3. This system of purified components was applied to the titration of the guinea-pig and human factors D and B in various homologous and heterologous reaction mixtures. On the basis of functional as well as of immunological tests, the D enzymes of both species were found to be related proteins. First, titrations of factor B revealed that both D enzymes activate homologous and heterologous B with comparable efficiency. Secondly, a rabbit anti-D antiserum raised against guinea-pig D was found to form a precipitation line of partial identity with guinea-pig and human D. In contrast to the compatibility between heterologous D and B, heterologous combinations of C3b and B were found to be incompatible. This incompatibility was indicated by low titres of D in the presence of heterologous C3b and B; in contrast, in the presence of homologous C3b and B, the titres of D were up to one hundred-fold higher. The reason for this effect was found to reside in inefficient complex formation between heterologous C3b and B. Therefore titrations of D and B should be only performed in the presence of homologous C3b and B.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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