Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1976 Feb;30(2):281–288.

The detection of F(ab')2-related surface antigens on the thymocytes of children.

V E Jones, H E Graves, E Orlans
PMCID: PMC1445001  PMID: 1083374

Abstract

A Fowl antiserum to human polyclonal F(ab')2 with specificities for variable region antigens of both light and heavy chains and for the constant portion of the Fd fragment bound to about 20 per cent of thymocytes from three children. Binding was completely inhibited by purified human polyclonal IgG and only partly by L chains. The fowl anti-F(ab')2 also bound to a subpopulation of peripheral blood lymphocytes which was somewhat larger than the B-cell population identified with other anti-immunoglobulin sera. Binding was detected by autoradiography and the amount of radiolabel seen on thymocytes was nearly as great as that on peripheral blood lymphocytes. As no binding to thymocytes was seen with other anti-immunoglobulin sera we propose that the fowl anti-F(ab')2 recognizes immunoglobulin or material with structural similarities which is synthesized by thymocytes but of which predominantly V region determinants are exposed on the membrane surface.

Full text

PDF
281

Images in this article

285Figure 1
on p.285

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Binz H., Kimura A., Wigzell H. Idiotype-positive T lymphocytes. Scand J Immunol. 1975 Sep;4(5-6):413–420. doi: 10.1111/j.1365-3083.1975.tb02646.x. [DOI] [PubMed] [Google Scholar]
  2. Burckhardt J. J., Guggisberg E., von Fellenberg R. Thymus immunoglobulin receptors. Ontogenic development, response to antigenic stimulation and their possible role in the regulation of "aggressive" immune reactions. Immunology. 1974 Mar;26(3):521–538. [PMC free article] [PubMed] [Google Scholar]
  3. Du Pasquier L., Weiss N., Loor F. Direct evidence for immunoglobulins on the surface of thymus lymphocytes of amphibian larvae. Eur J Immunol. 1972 Aug;2(4):366–370. doi: 10.1002/eji.1830020414. [DOI] [PubMed] [Google Scholar]
  4. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Goldstein G. Plasma cells in the human thymus. Aust J Exp Biol Med Sci. 1966 Dec;44(6):695–699. doi: 10.1038/icb.1966.66. [DOI] [PubMed] [Google Scholar]
  6. Grey H. M., Colon S., Solomon A., McLaughlin C. L. Immunoglobulin on the surface of human lymphocytes. The detection of kappa chain V region determinants on B cells and not on T cells. J Immunol. 1973 Dec;111(6):1923–1925. [PubMed] [Google Scholar]
  7. Haustein D., Marchalonis J. J., Crumpton M. J. Immunoglobulin of T lymphoma cells is an integral membrane protein. Nature. 1974 Dec 13;252(5484):602–604. doi: 10.1038/252602a0. [DOI] [PubMed] [Google Scholar]
  8. Hunt S. V., Williams A. F. The origin of cell surface immunoglobulin of marrow-derived and thymus-derived lymphocytes of the rat. J Exp Med. 1974 Mar 1;139(3):479–496. doi: 10.1084/jem.139.3.479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hämmerling G. J., McDevitt H. O. Antigen binding T and B lymphocytes. II. Studies on the inhibition of antigen binding to T and B cells by anti-immunoglobulin and anti-H-2 sera. J Immunol. 1974 May;112(5):1734–1740. [PubMed] [Google Scholar]
  10. Marchalonis J. J., Cone R. E. Biochemical and biological characteristics of lymphocyte surface immunoglobulin. Transplant Rev. 1973;14:3–49. doi: 10.1111/j.1600-065x.1973.tb00101.x. [DOI] [PubMed] [Google Scholar]
  11. Moroz C., Hahn Y. Cell-surface immunoglobulin human thymus cells and its biosynthesis in vitro. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3716–3720. doi: 10.1073/pnas.70.12.3716. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nossal G. J., Warner N. L., Lewis H., Sprent J. Quantitative features of a sandwich radioimmunolabeling technique for lymphocyte surface receptors. J Exp Med. 1972 Feb 1;135(2):405–428. doi: 10.1084/jem.135.2.405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Orlans E., Pearce C. A. Antigenic determinants in the N-terminal halves of human kappa-chains; their relation to the variable region subgroups. Eur J Immunol. 1974 Feb;4(2):86–90. doi: 10.1002/eji.1830040206. [DOI] [PubMed] [Google Scholar]
  14. Pernis B., Forni L., Amante L. Immunoglobulins as cell receptors. Ann N Y Acad Sci. 1971 Dec 31;190:420–431. doi: 10.1111/j.1749-6632.1971.tb13552.x. [DOI] [PubMed] [Google Scholar]
  15. Roelants G. E., Rydén A., Hägg L. B., Loor F. Active synthesis of immunoglobulin receptors for antigen by T lymphocytes. Nature. 1974 Jan 11;247(5436):106–108. doi: 10.1038/247106a0. [DOI] [PubMed] [Google Scholar]
  16. Warner N. L. Membrane immunoglobulins and antigen receptors on B and T lymphocytes. Adv Immunol. 1974;19(0):67–216. doi: 10.1016/s0065-2776(08)60252-7. [DOI] [PubMed] [Google Scholar]
  17. Wilson J. D., Nossal G. J. Identification of human T and B lymphocytes in normal peripheral blood and in chronic lymphocytic leukaemia. Lancet. 1971 Oct 9;2(7728):788–791. doi: 10.1016/s0140-6736(71)92741-3. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES