Abstract
Primary and booster IgE antibody responses have been elicited in Hooded Lister rats by the intradermal injection or oral administration of very small quantities of egg albumin. Oral immunization was effected by giving antigen by stomach tube or in the drinking water. The minimum primary dose of antigen found to be effective was 1 mug intradermally and 10 mug orally, administered together with an intraperitoneal injection of B. pertussis adjuvant. In rats immunized with these doses secondary responses could be evoked by giving even smaller quantities of antigen, thus 1 ng intradermally or 1 mug orally without adjuvant. Smaller challenge doses were not tried. Large primary doses of antigen (greater than 100 mug) presented by these routes were, on the other hand, found to be inhibitory to the production of secondary IgE responses, this effect being similar to that observed in previously reported intraperitoneal immunization experiments. By contrast with previous experiments, however, tertiary responses could be obtained following immunization by these routes, and we believe this to be reflection of the absorption of smaller and therefore less inhibitory quantities of antigen. Our results are discussed in relation to the control of IgE antibody production, current concepts of the control of antigen absorption through mucosal barriers, and possible implications of the genesis of naturally occurring IgE responses in man.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bazin H., Platteau B. Production of circulating reaginic (IgE) antibodies by oral administration of ovalbumin to rats. Immunology. 1976 May;30(5):679–684. [PMC free article] [PubMed] [Google Scholar]
- Bernstein I. D., Ovary Z. Absorption of antigens from the gastrointestinal tract. Int Arch Allergy Appl Immunol. 1968;33(6):521–527. doi: 10.1159/000230068. [DOI] [PubMed] [Google Scholar]
- Jarrett E. E., Stewart D. C. Rat IgE production. I. Effect of dose of antigen on primary and secondary reaginic antibody responses. Immunology. 1974 Sep;27(3):365–381. [PMC free article] [PubMed] [Google Scholar]
- Korenblat P. E., Rothberg R. M., Minden P., Farr R. S. Immune responses of human adults after oral and parenteral exposure to bovine serum albumin. J Allergy. 1968 Apr;41(4):226–235. doi: 10.1016/0021-8707(68)90046-4. [DOI] [PubMed] [Google Scholar]
- Levine B. B., Vaz N. M. Effect of combinations of inbred strain, antigen, and antigen dose on immune responsiveness and reagin production in the mouse. A potential mouse model for immune aspects of human atopic allergy. Int Arch Allergy Appl Immunol. 1970;39(2-3):156–171. doi: 10.1159/000230343. [DOI] [PubMed] [Google Scholar]
- PARKINS R. A., DIMITRIADOU A., BOOTH C. C. The rates and sites of absorption of 131 I-labelled albumin and sodium 131 I in the rat. Clin Sci. 1960 Nov;19:595–604. [PubMed] [Google Scholar]
- Taylor B., Norman A. P., Orgel H. A., Stokes C. R., Turner M. W., Soothill J. F. Transient IgA deficiency and pathogenesis of infantile atopy. Lancet. 1973 Jul 21;2(7821):111–113. doi: 10.1016/s0140-6736(73)93060-2. [DOI] [PubMed] [Google Scholar]