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. 1976 Dec;31(6):961–968.

Purification of cobra venom factor from phospholipase A contaminant.

P J Lachmann, L Halbwachs, A Gewurz, H Gewurz
PMCID: PMC1445186  PMID: 992719

Abstract

It has been demonstrated that cobra venom factor prepared by the usual combination of ion exchange chromatography and sephadex gel filtration is contaminated by substantial amounts of a 'heavy' phospholipase A. The two activities may be separated by isoelectric focusing. Cobra venom factor focuses at pH between 5-75 and 6-75 whereas the phospholipase is all found at pH below 7-75. In certain test systems, particularly in vitro, and particularly where albumin concentrations are low, the contaminating phospholipase may produce effects that have been attributed to complement activation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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