Abstract
A method for the isolation of large numbers of almost pure human eosinophils from peripheral blood using hypague-ficoll density gradients, gelatin sedimentation and carbonyl iron is described. Eosinophils were obtained from patients with parasitic infections, allergic reactions, undiagnosed eosinophilias and normal volunteers. Erythrocyte (E) and erythrocyte-antibody-complement (EAC) rosettes were formed by a number of eosinophils.
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- Boyle W. An extension of the 51Cr-release assay for the estimation of mouse cytotoxins. Transplantation. 1968 Sep;6(6):761–764. doi: 10.1097/00007890-196809000-00002. [DOI] [PubMed] [Google Scholar]
- Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
- Day R. P. Eosinophil cell separation from human peripheral blood. Immunology. 1970 Jun;18(6):955–959. [PMC free article] [PubMed] [Google Scholar]
- Hubscher T. Role of the eosinophil in the allergic reactions. I. EDI-an eosinophil-derived inhibitor of histamine release. J Immunol. 1975 Apr;114(4):1379–1388. [PubMed] [Google Scholar]
- Jondal M., Holm G., Wigzell H. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972 Aug 1;136(2):207–215. doi: 10.1084/jem.136.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sher R., Holm G., Kok S. H., Koornhof H. J., Glover A. T and CR+ lymphocyte profile in leprosy and the effect of treatment. Infect Immun. 1976 Jan;13(1):31–35. doi: 10.1128/iai.13.1.31-35.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]