Skip to main content
Immunology logoLink to Immunology
. 1977 May;32(5):709–715.

Heteroantisera prepared against B lymphocytes at different stages of differentiation. I. Preparation of sera and cytotoxicity to lymphoid cells from different organs.

R M Gorczynski
PMCID: PMC1445310  PMID: 301115

Abstract

A routine method is described whereby anti-mouse B-cell antisera can be raised in rabbits. As judged by trypan blue dye exclusion these sera have negligible cytotoxicity to murine T lymphocytes but seem to detect differences in non-T lymphocytes in different organs. By sedimentation analysis some of the sera may detect (in addition to mature B cells) earlier precursors of mature B lymphocytes in bone marrow and spleen.

Full text

PDF
709

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cantor H., Boyse E. A. Functional subclasses of T-lymphocytes bearing different Ly antigens. I. The generation of functionally distinct T-cell subclasses is a differentiative process independent of antigen. J Exp Med. 1975 Jun 1;141(6):1376–1389. doi: 10.1084/jem.141.6.1376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gorczynski R. M., Feldmann M. B Cell heterogeneity - difference in the size of B lymphocytes responding to T dependent and T independent antigens. Cell Immunol. 1975 Jul;18(1):88–97. doi: 10.1016/0008-8749(75)90039-8. [DOI] [PubMed] [Google Scholar]
  3. Gorczynski R. M., Miller R. G., Phillips R. A. Identification by density separation of antigen-specific surface receptors on the progenitors of antibody-producing cells. Immunology. 1971 May;20(5):693–705. [PMC free article] [PubMed] [Google Scholar]
  4. Gronowicz E., Coutinho A. Heterogeneity of B cells: direct evidence of selective triggering of distinct subpopulations by polyclonal activators. Scand J Immunol. 1976;5(1-2):55–69. doi: 10.1111/j.1365-3083.1976.tb02992.x. [DOI] [PubMed] [Google Scholar]
  5. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  6. Lafleur L., Miller R. G., Phillips R. A. A quantitative assay for the progenitors of bone marrow-associated lymphocytes. J Exp Med. 1972 Jun 1;135(6):1363–1374. doi: 10.1084/jem.135.6.1363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Miller R. G., Gorczynski R. M., Lafleur L., MacDonald H. R., Phillips R. A. Cell separation analysis of B and T lymphocyte differentiation. Transplant Rev. 1975;25:59–97. doi: 10.1111/j.1600-065x.1975.tb00726.x. [DOI] [PubMed] [Google Scholar]
  8. Miller R. G., Phillips R. A. Separation of cells by velocity sedimentation. J Cell Physiol. 1969 Jun;73(3):191–201. doi: 10.1002/jcp.1040730305. [DOI] [PubMed] [Google Scholar]
  9. Osmond D. G., Nossal G. J. Differentiation of lymphocytes in mouse bone marrow. II. Kinetics of maturation and renewal of antiglobulin-binding cells studied by double labeling. Cell Immunol. 1974 Jul;13(1):132–145. doi: 10.1016/0008-8749(74)90233-0. [DOI] [PubMed] [Google Scholar]
  10. Parish C. R., Chilcott A. B. Functional significance of the complement receptors on B lymphocytes. Cell Immunol. 1975 Dec;20(2):290–303. doi: 10.1016/0008-8749(75)90106-9. [DOI] [PubMed] [Google Scholar]
  11. Raff M. Theta isoantigen as a marker of thymus-derived lymphocytes in mice. Nature. 1969 Oct 25;224(5217):378–379. doi: 10.1038/224378a0. [DOI] [PubMed] [Google Scholar]
  12. Schlesinger M. Antigens of the thymus. Prog Allergy. 1972;16:214–299. doi: 10.1159/000313173. [DOI] [PubMed] [Google Scholar]
  13. Shortman K., Jackson H. The differentiation of T lymphocytes. I. Proliferation kinetics and interrelationships of subpopulations of mouse thymus cells. Cell Immunol. 1974 May;12(2):230–246. doi: 10.1016/0008-8749(74)90075-6. [DOI] [PubMed] [Google Scholar]
  14. Shortman K. Separation methods for lymphocyte populations. Contemp Top Mol Immunol. 1974;3:161–203. doi: 10.1007/978-1-4684-2838-4_7. [DOI] [PubMed] [Google Scholar]
  15. Strober S. Immune function cell surface characteristics and maturation of B cell subpopulations. Transplant Rev. 1975;24:84–112. doi: 10.1111/j.1600-065x.1975.tb00166.x. [DOI] [PubMed] [Google Scholar]
  16. Takahashi T., Old L. J., McIntire K. R., Boyse E. A. Immunoglobulin and other surface antigens of cells of the immune system. J Exp Med. 1971 Oct 1;134(4):815–832. doi: 10.1084/jem.134.4.815. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Trizio D., Cudkowicz G. Separation of T and B lymphocytes by nylon wool columns: evaluation of efficacy by functional assays in vivo. J Immunol. 1974 Oct;113(4):1093–1097. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES