Abstract
CBA thymocytes were subjected to cocapping experiments using AThy-1.2 and choleragen. The finding that both structures showed a common ligant-induced redistribution implies that choleragen receptors of the cell membrane are associated with the thymocyte-brain molecule. Furthermore, it was found that the thymocyte-brain antigenic determinants on the thymocyte were not blocked by prior choleragen treatment. This suggests a spatial distance between these structures on the molecule.
Full text
PDF



Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Arndt R., Stark R., Klein P., Müller A., Thiele H. G. Solubilization and molecular characterization of membrane-bound antigens shared by thymocytes and brain. Eur J Immunol. 1976 May;6(5):333–340. doi: 10.1002/eji.1830060506. [DOI] [PubMed] [Google Scholar]
- Atwell J. L., Cone R. E., Marchalonis J. J. Isolation of theta antigen from the surface of thymus lymphocytes. Nat New Biol. 1973 Feb 21;241(112):251–252. doi: 10.1038/newbio241251a0. [DOI] [PubMed] [Google Scholar]
- Cuatrecasas P. Gangliosides and membrane receptors for cholera toxin. Biochemistry. 1973 Aug 28;12(18):3558–3566. doi: 10.1021/bi00742a032. [DOI] [PubMed] [Google Scholar]
- Esselman W. J., Miller H. C. Brain and thymus lipid inhibition of antibrain-associated theta-cytotoxicity. J Exp Med. 1974 Feb 1;139(2):445–450. doi: 10.1084/jem.139.2.445. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Holmgren J., Lindholm L., Lönnroth I. Interaction of cholera toxin and toxin derivatives with lymphocytes. I. Binding properties and interference with lectin-induced cellular stimulation. J Exp Med. 1974 Apr 1;139(4):801–819. doi: 10.1084/jem.139.4.801. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Johnson B. J., Kucich U. N., Maurelli A. T. Studies on the antigenic determinants of the Thy-1.2 alloantigen as expressed by the murine lymphoblastoid line S-49.1 TB-2-3. J Immunol. 1976 Jun;116(6):1669–1672. [PubMed] [Google Scholar]
- Kucich U. N., Bennett J. C., Johnson B. J. The protein nature of the Thy-1.2 alloantigen as expressed by the murin lymphoblastoid line S-49.1 TB-2-3. J Immunol. 1975 Sep;115(3):626–630. [PubMed] [Google Scholar]
- Morris R. J., Letarte-Muirhead M., Williams A. F. Analysis in deoxycholate of three antigenic specificities associated with the rat Thy-1 molecule. Eur J Immunol. 1975 Apr;5(4):282–285. doi: 10.1002/eji.1830050413. [DOI] [PubMed] [Google Scholar]
- Muirhead M. L., Action R. T., Williams A. F. Preliminary characterization of Thy-1.1 and Ag-B antigens from rat tissues solubilized in detergents. Biochem J. 1974 Oct;143(1):51–61. doi: 10.1042/bj1430051. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Révész T., Greaves M. Ligand-induced redistribution of lymphocyte membrane ganglioside GM1. Nature. 1975 Sep 11;257(5522):103–106. doi: 10.1038/257103a0. [DOI] [PubMed] [Google Scholar]
- Stein-Douglas K., Schwarting G. A., Naiki M., Marcus D. M. Gangliosides as markers for murine lymphocyte subpopulations. J Exp Med. 1976 Apr 1;143(4):822–832. doi: 10.1084/jem.143.4.822. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thiele H. G., Stark R. Common antibody-induced redistribution on thymocytes of strain-, species- and non-species-specific antigenic determinants shared by brain and thymocytes of mice. Immunology. 1974 Nov;27(5):807–813. [PMC free article] [PubMed] [Google Scholar]
- Thiele H. G., Stark R., Keeser D. Antigenic correlations between brain and thymus. I. Common antigenic structures in rat and mouse brain tissue and thymocytes. Eur J Immunol. 1972 Oct;2(5):424–429. doi: 10.1002/eji.1830020508. [DOI] [PubMed] [Google Scholar]
- Vitetta E. S., Boyse E. A., Uhr J. W. Isolation and characterization of a molecular complex containing Thy-1 antigen from the surface of murine thymocytes and T cells. Eur J Immunol. 1973 Jul;3(7):446–453. doi: 10.1002/eji.1830030714. [DOI] [PubMed] [Google Scholar]