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. 1976 Oct;31(4):659–664.

Immunological studies of human placentae: subclass and fragment specificity of binding of aggregated IgG by placental endothelial cells.

P M Johnson, W P Faulk, A C Wang
PMCID: PMC1445366  PMID: 789232

Abstract

Fluorescein-conjugated heat-aggregated human IgG binds to endothelial cells of foetal stem vessels in cryostat sections of normal, full-term human placentae. No binding was observed using native human IgG of heat-aggregated human albumin, IgM or IgA2. No inhibition of binding of heat-aggregated human IgG was observed by pre-treatment of placental tissue sections with native IgG or non-aggregated Fc fragments. The binding was blocked using heat-aggregated Fc fragments prepared from IgG1, IgG2, IgG3 and IgG4 myeloma proteins, but not with heat-aggregated human light chains, Fab and F(ab)2 fragments of human IgG, or with heat-aggregated human IgM and IgA2. It is suggested that the placental endothelial cell receptor for aggregated IgG may function to keep immune complexes from entering the foetal circulation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aarli J. A., Aparicio S. R., Lumsden C. E., Tönder O. Binding of normal human IgG to myelin sheaths, glia and neurons. Immunology. 1975 Jan;28(1):171–185. [PMC free article] [PubMed] [Google Scholar]
  2. Aarli J. A., Tönder O. Fc binding of IgG to skeletal muscle tissue. Int Arch Allergy Appl Immunol. 1974;47(2):273–284. doi: 10.1159/000231220. [DOI] [PubMed] [Google Scholar]
  3. Dickler H. B., Kunkel H. G. Interaction of aggregated -globulin with B lymphocytes. J Exp Med. 1972 Jul 1;136(1):191–196. doi: 10.1084/jem.136.1.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FUDENBERG H. H., FUDENBERG B. R. ANTIBODY TO HEREDITARY HUMAN GAMMA-GLOBULIN (GM) FACTOR RESULTING FROM MATERNAL-FETAL INCOMPATIBILITY. Science. 1964 Jul 10;145(3628):170–171. doi: 10.1126/science.145.3628.170. [DOI] [PubMed] [Google Scholar]
  5. Faulk W. P., van Loghem E., Stickler G. B. Maternal antibody to fetal light chain (Inv) antigens. Am J Med. 1974 Mar;56(3):393–397. doi: 10.1016/0002-9343(74)90621-4. [DOI] [PubMed] [Google Scholar]
  6. Froland S. S., Michaelsen T. E., Wisloff F., Natvig J. B. Specificity of receptors for IgG on human lymphocyte-like cells. Scand J Immunol. 1974;3(4):509–517. doi: 10.1111/j.1365-3083.1974.tb01284.x. [DOI] [PubMed] [Google Scholar]
  7. Froland S. S., Natvig J. B., Michaelsen T. E. Binding of aggregated IgG BY HUMAN B lymphocytes independent of Fc receptors. Scand J Immunol. 1974;3(3):375–380. doi: 10.1111/j.1365-3083.1974.tb01268.x. [DOI] [PubMed] [Google Scholar]
  8. Hay F. C., Hull M. G., Torrigiani G. The transfer of human IgG subclasses from mother to foetus. Clin Exp Immunol. 1971 Sep;9(3):355–358. [PMC free article] [PubMed] [Google Scholar]
  9. Henson P. M., Spiegelberg H. L. Release of serotonin from human platelets induced by aggregated immunoglobulins of different classes and subclasses. J Clin Invest. 1973 May;52(5):1282–1288. doi: 10.1172/JCI107296. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Johnson P. M., Papamichail M., Gutierrez C., Holborow E. J. Interaction of the hinge region of human immunoglobulin G with a murine lymphocyte membrane receptor. Relevance to the problem of antiglobulin induction in rheumatoid arthritis. Immunology. 1975 May;28(5):797–805. [PMC free article] [PubMed] [Google Scholar]
  11. Johnson P. M., Trenchev P., Faulk W. P. Immunological studies of human placentae. Binding of complexed immunoglobulin by stromal endothelial cells. Clin Exp Immunol. 1975 Oct;22(1):133–138. [PMC free article] [PubMed] [Google Scholar]
  12. Johnson P. M., Watkins J., Scopes P. M., Tracey B. M. Differences in serum IgG structure in health and rheumatoid disease. Circular dichroism studies. Ann Rheum Dis. 1974 Jul;33(4):366–370. doi: 10.1136/ard.33.4.366. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lawrence D. A., Weigle W. O., Spiegelberg H. L. Immunoglobulins cytophilic for human lymphocytes, monocytes, and neutrophils. J Clin Invest. 1975 Feb;55(2):368–376. doi: 10.1172/JCI107940. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Matre R., Tönder O., Endresen C. Fc receptors in human placenta. Scand J Immunol. 1975;4(7):741–745. doi: 10.1111/j.1365-3083.1975.tb02682.x. [DOI] [PubMed] [Google Scholar]
  15. McCormick J. N., Faulk W. P., Fox H., Fudenberg H. H. Immunohistological and elution studies of the human placenta. J Exp Med. 1971 Jan 1;133(1):1–18. doi: 10.1084/jem.133.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Michaelsen T. E., Natvig J. B. Characterization of subclass-related F(ab)2, Fab-c and Fch fragments obtainined by short papain digestion of human IgG myeloma proteins. Scand J Immunol. 1973;2(3):299–312. doi: 10.1111/j.1365-3083.1973.tb02039.x. [DOI] [PubMed] [Google Scholar]
  17. PORTER R. R. The hydrolysis of rabbit y-globulin and antibodies with crystalline papain. Biochem J. 1959 Sep;73:119–126. doi: 10.1042/bj0730119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sachs D. H., Dickler H. B. The possible role of i region determined cell surface molecules in the regulation of immune responses. Transplant Rev. 1975;23:159–175. doi: 10.1111/j.1600-065x.1975.tb00156.x. [DOI] [PubMed] [Google Scholar]
  19. Spiegelberg H. L. Biological activities of immunoglobulins of different classes and subclasses. Adv Immunol. 1974;19(0):259–294. doi: 10.1016/s0065-2776(08)60254-0. [DOI] [PubMed] [Google Scholar]
  20. Tonder O., Morse P. A., Jr, Humphrey L. J. Similarities of Fc receptors in human malignant tissue and normal lymphoid tissue. J Immunol. 1974 Oct;113(4):1162–1169. [PubMed] [Google Scholar]
  21. Wang A. C., Faulk W. P., Stuckey M. A., Fudenberg H. H. Chemical differences of adult, fetal and hypogammaglobulinemic IgG immunoglobulins. Immunochemistry. 1970 Aug;7(8):703–708. doi: 10.1016/0019-2791(70)90176-x. [DOI] [PubMed] [Google Scholar]
  22. Wang A. C., Gergely J., Fudenberg H. H. Amino acid sequences at constant and variable regions of heavy chains of monotypic immunoglubulins G and M of a single patient. Biochemistry. 1973 Jan 30;12(3):528–534. doi: 10.1021/bi00727a027. [DOI] [PubMed] [Google Scholar]
  23. Weissmann G., Brand A., Franklin E. C. Interaction of immunoglobulins with liposomes. J Clin Invest. 1974 Feb;53(2):536–543. doi: 10.1172/JCI107587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wisloff F., Froland S. S., Michaelsen T. E. Antibody-dependent cytotoxicity mediated by human Fc-receptor-bearing cells lacking markers for B- and T-lymphocytes. Int Arch Allergy Appl Immunol. 1974;47(1):139–154. doi: 10.1159/000231208. [DOI] [PubMed] [Google Scholar]

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