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. 1974 Aug;27(2):271–283.

Passive cutaneous anaphylaxis and its enhancement by normal IgG

M R Williams
PMCID: PMC1445577  PMID: 4421606

Abstract

Rats were injected intradermally with rabbit anti-ovalbumin serum and 3 hours later were challenged intravenously with ovalbumin and Evans Blue dye. Inflammatory lesions were produced within 20 minutes and their size was markedly dose-dependent. Attempts were made to interfere with this passive cutaneous anaphylaxis (PCA) by admixture of normal IgG with the rabbit anti-ovalbumin to measure the relative tissue binding affinities of IgG from various species. It was found that normal IgG from any of the species tested had an enhancing effect on PCA in rats. These immunoglobulins serially arranged in the order of their decreasing enhancing abilities were: bovine>guinea-pig>pig>rat>rabbit>horse>human. The extent and significance of the enhancement increased considerably with increasing concentration of IgG in the test doses. The mechanism of enhancement was investigated and a hypothesis was proposed to explain the phenomenon.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALUND O., OSEBOLD J. W., MURPHY F. A. ISOLATION AND CHARACTERIZATION OF OVINE GAMMA GLOBULINS. Arch Biochem Biophys. 1965 Jan;109:142–149. doi: 10.1016/0003-9861(65)90299-7. [DOI] [PubMed] [Google Scholar]
  2. BIOZZI G., HALPERN B. N., BINAGHI R. The competitive effect of normal serum proteins from various animal species on antibody fixation in passive cutaneous anaphylaxis in the guinea pig. J Immunol. 1959 Mar;82(3):215–218. [PubMed] [Google Scholar]
  3. BRAMBELL F. W., HEMMINGS W. A., MORRIS I. G. A THEORETICAL MODEL OF GAMMA-GLOBULIN CATABOLISM. Nature. 1964 Sep 26;203:1352–1354. doi: 10.1038/2031352a0. [DOI] [PubMed] [Google Scholar]
  4. FISHER J. P., COOKE R. A. Passive cutaneous anaphylaxis (PCA) in the guinea pig; an immunologic and pathologic study. J Allergy. 1957 Mar;28(2):150–169. doi: 10.1016/0021-8707(57)90021-7. [DOI] [PubMed] [Google Scholar]
  5. Goose J., Blair A. M. Passive cutaneous anaphylaxis in the rat, induced with two homologous reagin-like antibodies and its specific inhibition with disodium cromoglycate. Immunology. 1969 Jun;16(6):749–760. [PMC free article] [PubMed] [Google Scholar]
  6. HARTLEY P. The effect of peptic digestion on the properties of diphtheria antitoxin. Proc R Soc Lond B Biol Sci. 1951 Oct 30;138(893):499–513. doi: 10.1098/rspb.1951.0037. [DOI] [PubMed] [Google Scholar]
  7. ISHIZAKA K., ISHIZAKA T. Biologic activity of aggregated gamma-globulin. II. A study of various methods for aggregation and species differences. J Immunol. 1960 Aug;85:163–171. [PubMed] [Google Scholar]
  8. Kekwick R. A. The serum proteins in multiple myelomatosis. Biochem J. 1940 Sep;34(8-9):1248–1257. doi: 10.1042/bj0341248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. King C. A., Francis G. E. Adsorption of heterologous gamma-globulins by chopped guinea-pig lung. Immunology. 1967 Jul;13(1):1–8. [PMC free article] [PubMed] [Google Scholar]
  10. Lovett C. A., Movat H. Z. Role of PMN-leukocyte lyosomes in tissue injury, inflammation and hypersensitivity. 3. Passive cutaneous anaphylaxis induced in the rat with homologous and heterologous hyperimmune antibody. Proc Soc Exp Biol Med. 1966 Aug-Sep;122(4):991–996. doi: 10.3181/00379727-122-31307. [DOI] [PubMed] [Google Scholar]
  11. MOTA I. Mast cells and anaphylaxis. Ann N Y Acad Sci. 1963 Feb 26;103:264–277. doi: 10.1111/j.1749-6632.1963.tb53703.x. [DOI] [PubMed] [Google Scholar]
  12. Movat H. Z., Lovett C. A., Taichman N. S. Demonstration of antigen on the surface of sensitized rat mast cells. Nature. 1966 Nov 19;212(5064):851–853. doi: 10.1038/212851b0. [DOI] [PubMed] [Google Scholar]
  13. OVARY Z., BLOCH K. J., BENACERRAF B. IDENTIFICATION OF RABBIT, MONKEY, AND DOG ANTIBODIES WITH PCA ACTIVITY FOR GUINEA PIGS. Proc Soc Exp Biol Med. 1964 Jul;116:840–845. doi: 10.3181/00379727-116-29388. [DOI] [PubMed] [Google Scholar]
  14. OVARY Z. [Cutaneous anaphylaxis in the albino rat]. Int Arch Allergy Appl Immunol. 1952;3(4):293–301. doi: 10.1159/000227977. [DOI] [PubMed] [Google Scholar]
  15. Orr T. S., Gwilliam J., Cox J. S. Studies on passive cutaneous anaphylaxis in the rat with disodium cromoglycate. II. A comparison of the rat anti-dnp 7S 2 and rat reagin induced cutaneous reactions. Immunology. 1971 Sep;21(3):405–417. [PMC free article] [PubMed] [Google Scholar]
  16. SPIEGELBERG H. L., WEIGLE W. O. THE CATABOLISM OF HOMOLOGOUS AND HETEROLOGOUS 7S GAMMA GLOBULIN FRAGMENTS. J Exp Med. 1965 Mar 1;121:323–338. doi: 10.1084/jem.121.3.323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Webb T., Goodman H. C. The structure and function of immunoglobulins. Mod Trends Immunol. 1967;2:151–187. [PubMed] [Google Scholar]

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