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. 1974 Sep;27(3):441–455.

Specificity of cell-mediated transplantation reactions

II. Studies with a sensitive assay of cell-mediated cytotoxicity

P Lake, E Sabbadini, A H Sehon
PMCID: PMC1445607  PMID: 4137937

Abstract

To study the specificity of transplantation immunity in vitro the capacity of two aggressor cell populations from allografted mice to destroy target cells obtained from a panel of strains having different H-2 types was assessed. 51Chromium-labelled macrophages were found to be suitable target cells to allow for quantitative comparisons of cytotoxicity. Spleen cells from skin-grafted mice lysed only target cells of strains which shared at least one H-2 region (K or D) with the donor. By contrast, non-adherent peritoneal exudate cells, produced as a consequence of ascites tumour allografts, exhibited a cytotoxic potential approximately 16- to 32-fold greater than the above spleen cells and were capable of lysing third-party target cells of strains having disparity with the graft donor at both H-2 regions. The difference between the two systems appears to be quantitative. These findings appear to reconcile previously conflicting observations, and suggest minimal or no participation of public H-2 specificities in cell-mediated reactions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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