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. 1974 Dec;27(6):1103–1115.

Immunoglobulins of the marsupial Setonix brachyurus (Quokka)

Characterization of three major serum classes, IgG2, IgG1 and IgM

R G Bell, N R Lynch, K J Turner
PMCID: PMC1445692  PMID: 4141695

Abstract

Three immunoglobulin classes, IgG2, IgG1 and IgM, have been identified in the serum of the marsupial Setonix brachyurus (quokka). Each of these classes has been isolated in pure form and partially characterized physicochemically. These immunoglobulins differed in their electrophoretic mobility, molecular size, carbohydrate content and in the antigenic determinants of their heavy chains. IgG2 exists in two major subclasses, one of which (IgG2a) was isolated as a homogeneous preparation. The results of immunoelectrophoretic analyses also suggest the likely presence of subclasses within the IgG1 class. This molecular complexity of the quokka humoral immune system is much greater than that of birds and ectotherms, but is comparable to that shown by eutherian mammals.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BENACERRAF B., OVARY Z., BLOCH K. J., FRANKLIN E. C. Properties of guinea pig 7S antibodies. I. Electrophoretic separation of two types of guinea pig 7S antibodies. J Exp Med. 1963 Jun 1;117:937–949. doi: 10.1084/jem.117.6.937. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bell R. G., Stephens C. J., Turner K. J. Marsupial immunoglobulins: an immunoglobulin molecule resembling eutherian IgA in serum and secretions of Setonix brachyurus (quokka). J Immunol. 1974 Jul;113(1):371–378. [PubMed] [Google Scholar]
  3. FAHEY J. L., WUNDERLICH J., MISHELL R. THE IMMUNOGLOBULINS OF MICE. I. FOUR MAJOR CLASSES OF IMMUNOGLOBULINS: 7S GAMMA-2-, 7S GAMMA-1-, GAMMA-1A (BETA-2A)-, AND 18S GAMMA-1M-GLOBULINS. J Exp Med. 1964 Aug 1;120:223–242. doi: 10.1084/jem.120.2.223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FAHEY J. L., WUNDERLICH J., MISHELL R. THE IMMUNOGLOBULINS OF MICE. II. TWO SUBCLASSES OF MOUSE 7S GAMMA-2-GLOBULINS: GAMMA-2A- AND GAMMA-2B-GLOBULINS. J Exp Med. 1964 Aug 1;120:243–251. doi: 10.1084/jem.120.2.243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Grey H. M. Duck immunoglobulins. I. Structural studies on a 5.7S and 7.8S gamma-globulin. J Immunol. 1967 Apr;98(4):811–819. [PubMed] [Google Scholar]
  6. Johnson J. S., Vaughan J. H. Canine immunoglobulins. I. Evidence for six immunoglobulin classes. J Immunol. 1967 May;98(5):923–934. [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Leslie G. A., Clem L. W. Phylogen of immunoglobulin structure and function. 3. Immunoglobulins of the chicken. J Exp Med. 1969 Dec 1;130(6):1337–1352. doi: 10.1084/jem.130.6.1337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Leslie G. A., Martin L. N. Studies on the secretory immunologic system of fowl. 3. Serum and secretory IgA of the chicken. J Immunol. 1973 Jan;110(1):1–9. [PubMed] [Google Scholar]
  10. Litman G. W., Fromme D., Chartrand S. L., Finstad J., Good R. A. Significance of heavy chain mass and antigenic relationship in immunoglobulin evolution. Immunochemistry. 1971 Apr;8(4):345–349. doi: 10.1016/0019-2791(71)90156-x. [DOI] [PubMed] [Google Scholar]
  11. Marchalonis J. J., Edelman G. M. Phylogenetic origins of antibody structure. 3. Antibodies in the primary immune response of the sea lamprey, Petromyzon marinus. J Exp Med. 1968 May 1;127(5):891–914. doi: 10.1084/jem.127.5.891. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Marchalonis J. J. Isolation and characterization of immunoglobulin-like proteins of the Australian lungfish (Neoceratodus forsteri). Aust J Exp Biol Med Sci. 1969 Aug;47(4):405–419. doi: 10.1038/icb.1969.46. [DOI] [PubMed] [Google Scholar]
  13. Marchalonis J. J. Isolation and partial characterization of immunoglobulins of goldfish (Carassius auratus) and carp (Cyprinus carpio). Immunology. 1971 Feb;20(2):161–173. [PMC free article] [PubMed] [Google Scholar]
  14. Marx J. J., Jr, Burrell R., Fisher S. Q. A study of the afferent and efferent limbs of the immune response in opossums. J Immunol. 1971 Apr;106(4):1043–1049. [PubMed] [Google Scholar]
  15. ROWLEY D., TURNER K. J. INCREASE IN MACROGLOBULIN ANTIBODIES OF MOUSE AND PIG FOLLOWING INJECTION OF BACTERIAL LIPOPOLYSACCHARIDE. Immunology. 1964 Jul;7:394–402. [PMC free article] [PubMed] [Google Scholar]
  16. Rowlands D. T., Jr, Dudley M. A. The isolation of immunoglobulins of the adult opossum (Didelphys virginiana). J Immunol. 1968 Apr;100(4):736–743. [PubMed] [Google Scholar]
  17. Tabel H., Ingram D. G. Immunoglobulins of mink. Evidence for five immunoglobulin classes of 7S type. Immunology. 1972 Jun;22(6):933–942. [PMC free article] [PubMed] [Google Scholar]
  18. Thomas W. R., Turner K. J., Eadie M. E., Yadav M. The immune response of the quokka (Setonix brachyurus). The production of a low molecular weight antibody. Immunology. 1972 Mar;22(3):401–416. [PMC free article] [PubMed] [Google Scholar]

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