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. 1974 Dec;27(6):973–987.

Uptake and release of DNA by lymphoid tissue and cells

I Olsen, G Harris
PMCID: PMC1445696  PMID: 4452578

Abstract

Newly synthesized DNA has been shown to be released by immunized rabbit spleen tissue cultured in vitro. This DNA was mainly doublestranded, showed a large spread in buoyant density, and was in the molecular weight range 50,000–500,000. Rabbit spleen tissue and human peripheral blood lymphocytes in active DNA synthesis, also took up bacterial DNA into their nuclei. After short periods of culture this DNA had a buoyant density of the bacterial DNA employed. Upon prolonged incubation, the DNA was of mammalian density. Evidence was obtained, in the case of the blood lymphocytes, for the appearance in the cells of a DNA of intermediate buoyant density. The possible relevance of the loss and reutilization of DNA to lymphoid cell function is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anker P., Stroun M. Bacterial nature of radioactive DNA found in tomato plants incubated in the presence of bacterial DNA-3H. Nature. 1968 Aug 31;219(5157):932–933. doi: 10.1038/219932a0. [DOI] [PubMed] [Google Scholar]
  2. Anker P., Stroun M. Bacterial ribonucleic acid in the frog brain after a bacterial peritoneal infection. Science. 1972 Nov 10;178(4061):621–623. doi: 10.1126/science.178.4061.621. [DOI] [PubMed] [Google Scholar]
  3. Ayad S. R., Fox M. DNA uptake by a mutant strain of lymphoma cells. Nature. 1968 Oct 5;220(5162):35–38. doi: 10.1038/220035a0. [DOI] [PubMed] [Google Scholar]
  4. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Davila C., Charles P., Ledoux L. The chromatography of nucleic acid preparations on DEAE-cellulose paper. I. Fractionation of deoxyribonucleic acid on paper strips or on centrifuged paper pulp. J Chromatogr. 1965 Aug;19(2):382–395. doi: 10.1016/s0021-9673(01)99474-9. [DOI] [PubMed] [Google Scholar]
  6. Dutton R. W. Further studies of the stimulation of DNA synthesis in cultures of spleen cell suspensions by homologous cells in inbred strains of mice and rats. J Exp Med. 1965 Oct 1;122(4):759–770. doi: 10.1084/jem.122.4.759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HANNA M. G., Jr AN AUTORADIOGRAPHIC STUDY OF THE GERMINAL CENTER IN SPLEEN WHITE PULP DURING EARLY INTERVALS OF THE IMMUNE RESPONSE. Lab Invest. 1964 Feb;13:95–104. [PubMed] [Google Scholar]
  8. Harris G., Pelc S. R., Blackmore D. K. Synthesis of DNA by the spleens of germ-free mice during the primary response to sheep red cells. Eur J Immunol. 1973 Feb;3(2):103–108. doi: 10.1002/eji.1830030210. [DOI] [PubMed] [Google Scholar]
  9. Harris G., Pelc S. R. Incorporation of [3H] thymidine into the spleens of intact mice during the immune response to sheep erythrocytes (SRC). Immunology. 1970 Dec;19(6):865–878. [PMC free article] [PubMed] [Google Scholar]
  10. Harris G. The immune response of spleen explants from primed rabbits to sheep red cells (SRC). I. DNA synthesis in the development of antibody-producing cells (PEC). Immunology. 1973 Feb;24(2):343–363. [PMC free article] [PubMed] [Google Scholar]
  11. Hill M., Hillova J. Recombinational events between exogenous mouse DNA and newly synthesized DNA strands of chicken cells in culture. Nat New Biol. 1971 Jun 30;231(26):261–265. doi: 10.1038/newbio231261a0. [DOI] [PubMed] [Google Scholar]
  12. Hill M., Huppert J. Fate of exogenous mouse DNA in chicken fibroblasts in vitro. Non-conservative preservation. Biochim Biophys Acta. 1970 Jul 16;213(1):26–35. doi: 10.1016/0005-2787(70)90004-3. [DOI] [PubMed] [Google Scholar]
  13. Hill M. The uptake of deoxyribonucleic acid released from damaged cells in tissue cultures. Exp Cell Res. 1967 Mar;45(3):533–549. doi: 10.1016/0014-4827(67)90158-9. [DOI] [PubMed] [Google Scholar]
  14. Ledoux L., Charles P. Uptake of exogenous DNA by mouse embryos. Exp Cell Res. 1967 Feb;45(2):498–501. doi: 10.1016/0014-4827(67)90200-5. [DOI] [PubMed] [Google Scholar]
  15. Ledoux L., Huart R. Fate of exogenous bacterial deoxyribonucleic acids in barley seedlings. J Mol Biol. 1969 Jul 28;43(2):243–262. doi: 10.1016/0022-2836(69)90265-4. [DOI] [PubMed] [Google Scholar]
  16. Ledoux L., Huart R., Jacobs M. Fate of exogenous DNA in Arabidopsis thaliana. Translocation and integration. Eur J Biochem. 1971 Nov 11;23(1):96–108. doi: 10.1111/j.1432-1033.1971.tb01596.x. [DOI] [PubMed] [Google Scholar]
  17. NOSSAL G. J., MAKELA O. Autoradiographic studies on the immune response.I. The kinetics of plasma cell proliferation. J Exp Med. 1962 Jan 1;115:209–230. doi: 10.1084/jem.115.1.209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. PELC S. R. LABELLING OF DNA AND CELL DIVISION IN SO CALLED NON-DIVIDING TISSUES. J Cell Biol. 1964 Jul;22:21–28. doi: 10.1083/jcb.22.1.21. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Pelc S. R., Harris G., Caldwell I. The relationship between antibody formation and deoxyribonucleic acid (DNA) synthesis in mouse spleen during primary and secondary response to sheep erythrocytes (SRC). Immunology. 1972 Aug;23(2):183–197. [PMC free article] [PubMed] [Google Scholar]
  20. Pelc S. R., Viola-Magni M. P. 3. Decrease of labeled DNA in cells of the adrenal medulla after intermittent exposure to cold. J Cell Biol. 1969 Aug;42(2):460–468. doi: 10.1083/jcb.42.2.460. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Robins A. B., Taylor D. M. Nuclear uptake of exogenous DNA by mammalian cells in culture. Nature. 1968 Mar 30;217(5135):1228–1231. doi: 10.1038/2171228a0. [DOI] [PubMed] [Google Scholar]
  22. Rogers J. C., Boldt D., Kornfeld S., Skinner A., Valeri C. R. Excretion of deoxyribonucleic acid by lymphocytes stimulated with phytohemagglutinin or antigen. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1685–1689. doi: 10.1073/pnas.69.7.1685. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Stroun M., Anker P., Gahan P., Rossier A., Greppin H. Agrobacterium tumefaciens ribonucleic acid synthesis in tomato cells and crown gall induction. J Bacteriol. 1971 May;106(2):634–639. doi: 10.1128/jb.106.2.634-639.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Stroun M., Anker P. In vitro synthesis of DNA spontaneously released by bacteria or frog auricles. Biochimie. 1972;54(11):1443–1452. doi: 10.1016/s0300-9084(72)80086-5. [DOI] [PubMed] [Google Scholar]
  25. Stroun M., Charles P., Anker P., Pelc S. R. Metabolic DNA in heart and skeletal muscle and in the intestine of mice. Nature. 1967 Nov 18;216(5116):716–717. doi: 10.1038/216716a0. [DOI] [PubMed] [Google Scholar]
  26. Stroun M., Gahan P., Sarid S. Agrobacterium tumefaciens RNA in non-tumorous tomato cells. Biochem Biophys Res Commun. 1969 Nov 6;37(4):652–657. doi: 10.1016/0006-291x(69)90860-2. [DOI] [PubMed] [Google Scholar]

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