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. 1974 Oct;27(4):589–599.

Cytophilic activity of IgG2 from sera of guinea-pigs immunized with bovine γ-globulin

R G Q Leslie, S Cohen
PMCID: PMC1445719  PMID: 4140145

Abstract

IgG2 from immune guinea-pig sera collected 1-2 weeks after secondary BGG challenge showed considerably enhanced cytophilic activity; the preparations contained aggregates (±10 per cent of the total immunoglobulin) shown by antigen exchange to be immune complexes with a probable composition Ag (fragments) Ab5. After removal of aggregates the cytophilic activity of immune 7S IgG2 approximated that of IgG2 from unimmunized animals.

Artificial immune complexes from either non-immune or immune 7S IgG2 (aggregated with the F(ab')2 fragments of rabbit anti-guinea-pig Fab) showed greatly enhanced cytophilic activity, maximal at antigen: antibody equivalence and comparable for both IgG2 preparations.

These data are consistent with the view that cytophilic activity for macrophages is a uniform property of the whole guinea-pig IgG2 class.

The IgG2 complexes apparently bind to glass-adherent cells and also to a population of non-glass-adherent mononuclear cells, present in peritoneal exudates but not detectable in peripheral blood.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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