Abstract
The binding of normal human serum, purified IgG and IgG fragments to central nervous tissue was studied by the anti-globulin consumption (AGCT) and immunofluorescence (IF) techniques. In the AGCT, F(ab')2 fragments failed to react, whereas IgG and Fc fragments did so. In IF experiments, the binding was localized to myelin sheaths, glia and neurons; Fab monomers at a protein concentration of 1-3 mg/ml dod not react with the tissue, but purified Fc fragments at 0-0625 mg/ml did. The binding is neither tissue- nor species-specific. Lipid and protein extraction procedures indicated that the factor responsible for binding to myelin was basic protein. It was concluded that the binding of normal IgG to central nervous tissue is medicated by the Fc part of the molecule.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ALLERAND C. D., YAHR M. D. GAMMA-GLOBULIN AFFINITY FOR NORMAL HUMAN TISSUE OF THE CENTRAL NERVOUS SYSTEM. Science. 1964 May 29;144(3622):1141–1147. doi: 10.1126/science.144.3622.1141. [DOI] [PubMed] [Google Scholar]
- AUTILIO L. A., NORTON W. T., TERRY R. D. THE PREPARATION AND SOME PROPERTIES OF PURIFIED MYELIN FROM THE CENTRAL NERVOUS SYSTEM. J Neurochem. 1964 Jan;11:17–27. doi: 10.1111/j.1471-4159.1964.tb06719.x. [DOI] [PubMed] [Google Scholar]
- Aarli J. A., Tönder O. Antiglobulin consumption test with sera from patients with myasthenia gravis. Clin Exp Immunol. 1970 Jul;7(1):11–21. [PMC free article] [PubMed] [Google Scholar]
- BEUTNER E. H., WITEBSKY E., ROSE N. R., GERBASI J. R. Localization of thyroid and spinal cord autoantibodies by fluorescent antibody technic. Proc Soc Exp Biol Med. 1958 Mar;97(3):712–716. doi: 10.3181/00379727-97-23855. [DOI] [PubMed] [Google Scholar]
- Edgington T. S., Dalessio D. J. The assessment by immunofluorescence methods of humoral anti-myelin antibodies in man. J Immunol. 1970 Jul;105(1):248–255. [PubMed] [Google Scholar]
- FAHEY J. L., MCLAUGHLIN C. PREPARATION OF ANTISERA SPECIFIC FOR 6.6 S GAMMA-GLOBULINS, BETA 2A-GLOBULINS, GAMMA-1.-MACROGLOBULINS, AND FOR TYPE I AND II COMMON GAMMA-GLOBULIN DETERMINANTS. J Immunol. 1963 Oct;91:484–497. [PubMed] [Google Scholar]
- Lumsden C. E. The immunogenesis of the multiple sclerosis plaque. Brain Res. 1971 May 21;28(3):365–390. doi: 10.1016/0006-8993(71)90052-7. [DOI] [PubMed] [Google Scholar]
- Sanderson A. R., Lanning M. G. Crystalline Fc prepared in high yield from normal human IgG. Nature. 1970 Apr 25;226(5243):356–358. doi: 10.1038/226356b0. [DOI] [PubMed] [Google Scholar]
- Spiegelberg H. L., Weigle W. O. The immune response to human gamma-G-immunoglobulin in rabbits unresponsive to Fc fragment and H chain protein. J Immunol. 1967 May;98(5):1020–1027. [PubMed] [Google Scholar]
- Summers D. F., Maizel J. V., Jr, Darnell J. E., Jr Evidence for virus-specific noncapsid proteins in poliovirus-infected HeLa cells. Proc Natl Acad Sci U S A. 1965 Aug;54(2):505–513. doi: 10.1073/pnas.54.2.505. [DOI] [PMC free article] [PubMed] [Google Scholar]