Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1975 Apr;28(4):693–702.

The haemopoietic and immunogenic capacities of living hybrid bone marrow cells tested in tumour allograft rejection.

J A Kerckhaert, F M Hofhuis, J M Willers
PMCID: PMC1445819  PMID: 1150311

Abstract

In irradiated mice the capacity to reject allogenic tumours can be reconstituted with syngeneic lymphoid cells if the transferred cells are primed with the allogenicantigen. Living semi-allogeneic cells proved to be 30-100-times more acitve as priming antigen than cell membrane fractions. The tumour-suppressive activity of primed lymphoid cells increased in the following order: bone marrow less than Peyer's patches less than thymus less than spleen less than lymph node cells. Even bone marrowcells showed a considerable suppressing activity after priming with live antigen. It was a great advantage that 2 times 10-6 semi-allogeneic bone marrow cells could be used both for the restoration of the haemopoietic system after irradiation and for stimulation of the transferred parental lymphocytes. Priming with large numbers of semi-allogeneic spleen cells abolished the tumour-suppressive activity of the transferred lymphoid cells. This tolerogenic effect disappeared when the priming cells were pretreated with mitomycin. Tolerance could be induced when the cell donors were treated with cyclophosphamide in combination with the living cells. Cell membranes were not effective.

Full text

PDF
693

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aisenberg A. C., Wilkes B. Immunological tolerance induced by cyclophosphamide assayed by plaque spleen cell method. Nature. 1967 Feb 4;213(5075):498–499. doi: 10.1038/213498a0. [DOI] [PubMed] [Google Scholar]
  2. Berke G., Amos D. B. Cytotoxic lymphocytes in the absence of detectable antibody. Nat New Biol. 1973 Apr 25;242(121):237–239. doi: 10.1038/newbio242237a0. [DOI] [PubMed] [Google Scholar]
  3. Blomgren H., Svedmyr E. In vitro stimulation of mouse thymus cells by PHA and allogeneic cells. Cell Immunol. 1971 Aug;2(4):285–299. doi: 10.1016/0008-8749(71)90063-3. [DOI] [PubMed] [Google Scholar]
  4. Bloom B. R. In vitro approaches to the mechanism of cell-mediated immune reactions. Adv Immunol. 1971;13:101–208. doi: 10.1016/s0065-2776(08)60184-4. [DOI] [PubMed] [Google Scholar]
  5. Davies A. J. The thymus and the cellular basis of immunity. Transplant Rev. 1969;1:43–91. doi: 10.1111/j.1600-065x.1969.tb00136.x. [DOI] [PubMed] [Google Scholar]
  6. Dehamer D. L., Goodman J. W. Origin of cells repopulating the spleens of irradiated mice given marrow cells and thymocytes. Transplantation. 1973 May;15(5):474–477. [PubMed] [Google Scholar]
  7. Freedman L. R., Cerottini J. C., Brunner K. T. In vivo studies of the role of cytotoxic T cells in tumor allograft immunity. J Immunol. 1972 Dec;109(6):1371–1378. [PubMed] [Google Scholar]
  8. IYER V. N., SZYBALSKI W. MITOMYCINS AND PORFIROMYCIN: CHEMICAL MECHANISM OF ACTIVATION AND CROSS-LINKING OF DNA. Science. 1964 Jul 3;145(3627):55–58. doi: 10.1126/science.145.3627.55. [DOI] [PubMed] [Google Scholar]
  9. Im H. M., Simmons R. L. Modification of graft-versus-host disease by neuraminidase treatment of donor cells. Decreased tolerogenicity of neuraminidase-treated cells. Transplantation. 1971 Dec;12(6):472–478. doi: 10.1097/00007890-197112000-00010. [DOI] [PubMed] [Google Scholar]
  10. Kerckhaert J. A., Benner R., Willers J. M. Cells involved in the graft-versus-host reaction in vitro. Immunology. 1973 Jul;25(1):103–110. [PMC free article] [PubMed] [Google Scholar]
  11. Kerckhaert J. A. Influence of cyclophosphamide on the delayed hypersensitivity in the mouse after intraperitoneal immunization. Ann Immunol (Paris) 1974 Jun;125 100(4):559–568. [PubMed] [Google Scholar]
  12. Kerckhaert J. A., Van den Berg G. J., Willers J. M. Influence of cyclophosphamide on the delayed hypersensitivity of the mouse. Ann Immunol (Paris) 1974 Mar-Apr;125(3):415–426. [PubMed] [Google Scholar]
  13. Lonai P., Clark W. R., Feldman M. Participation of theta-bearing cell in an in vitro assay of transplantation immunity. Nature. 1971 Feb 19;229(5286):566–567. doi: 10.1038/229566a0. [DOI] [PubMed] [Google Scholar]
  14. Mackaness G. B., Blanden R. V., Collins F. M. Host-parasite relations in mouse typhoid. J Exp Med. 1966 Oct 1;124(4):573–583. doi: 10.1084/jem.124.4.573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mekori T., Chieco-Bianci L., Feldman M. Production of clones of lymphoid cell populations. Nature. 1965 Apr 24;206(982):367–368. doi: 10.1038/206367a0. [DOI] [PubMed] [Google Scholar]
  16. Miller J. F., Mitchell G. F. Thymus and antigen-reactive cells. Transplant Rev. 1969;1:3–42. doi: 10.1111/j.1600-065x.1969.tb00135.x. [DOI] [PubMed] [Google Scholar]
  17. Möller G. Immunocyte triggering. Cell Immunol. 1970 Dec;1(6):573–582. doi: 10.1016/0008-8749(70)90023-7. [DOI] [PubMed] [Google Scholar]
  18. Parish C. R., Wistar R., Ada G. L. Cleavage of bacterial flagellin with cyanogen bromide. Antigenic properties of the protein fragments. Biochem J. 1969 Jul;113(3):501–506. doi: 10.1042/bj1130501. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Shevach E. M., Rosenstreich D. L., Green I. The distribution of histocompatibility antigens on T and B cells in the guinea pig. Transplantation. 1973 Aug;16(2):126–133. doi: 10.1097/00007890-197308000-00008. [DOI] [PubMed] [Google Scholar]
  20. Takada A., Takada Y. Role of the thymus in the proliferation and migration of donor marrow cells in irradiated allogeneic host mice. Transplantation. 1973 May;15(5):460–464. [PubMed] [Google Scholar]
  21. Veldkamp J., van der Gaag R., Willers J. M. The role of Peyer's patch cells in antibody formation. Immunology. 1973 Nov;25(5):761–771. [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES