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. 1975 Jul;29(1):121–130.

The radiosensitivity of T and B lymphocytes in mice.

Y Kataoka, T Sado
PMCID: PMC1445853  PMID: 124701

Abstract

The radiosensitivity of T and B lymphocytes in spleens of specific pathogen-free C3Hf/HeMs male mice was studied by the direct and indirect immunofluorescence technique. It was found that the radiobiological parameters characterizing the survival curve of Bpsi lymphocytes were DO = 200 R and n = 1-00. The T lymphocytes, on the other hand, were shown to consist of two distinct subpopulations with respect to their radiosensitivity. The radiobiological parameters of the radiosensitive fraction of T lymphocytes were Dq = 185 R, DO =195 R and n = 2-50. The DO value of the radioresistant T lymphocyte subpopulation was practically unmeasurable. It was estimated that approximately 8 per cent of the T lymphocytes present in the spleen of normal C3Hf mice belonged to this radioresistant subpopulation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aisenberg A. C., Davis C. The thymus and recovery of the sheep erythrocyte response in irradiated mice. J Exp Med. 1968 Dec 1;128(6):1327–1338. doi: 10.1084/jem.128.6.1327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Asherson G. L., Loewi G. The effect of irradiation on the passive transfer of delayed hypersensitivity. Immunology. 1967 Nov;13(5):509–512. [PMC free article] [PubMed] [Google Scholar]
  3. CELADA F., CARTER R. R. The radiosensitive nature of homograft-rejecting and agglutinin-forming capacities of isolated spleen cells. J Immunol. 1962 Aug;89:161–169. [PubMed] [Google Scholar]
  4. Claman H. N., Chaperon E. A. Immunologic complementation between thymus and marrow cells--a model for the two-cell theory of immunocompetence. Transplant Rev. 1969;1:92–113. doi: 10.1111/j.1600-065x.1969.tb00137.x. [DOI] [PubMed] [Google Scholar]
  5. Claman H. N., Chaperon E. A., Triplett R. F. Thymus-marrow cell combinations. Synergism in antibody production. Proc Soc Exp Biol Med. 1966 Aug-Sep;122(4):1167–1171. doi: 10.3181/00379727-122-31353. [DOI] [PubMed] [Google Scholar]
  6. FELDMAN M., GLOBERSON A. THE ROLE OF THE THYMUS IN RESTORING IMMUNOLOGICAL REACTIVITY AND LYMPHOID CELL DIFFERENTIATION IN X-IRRADIATED ADULT MICE. Ann N Y Acad Sci. 1964 Nov 30;120:182–190. doi: 10.1111/j.1749-6632.1964.tb34716.x. [DOI] [PubMed] [Google Scholar]
  7. Feldman J. D. The role of proliferation in delayed hypersensitivity. J Immunol. 1968 Sep;101(3):563–571. [PubMed] [Google Scholar]
  8. GLOBERSON A., FIORE-DONATI L., FELDMAN M. On the role of the thymus in recovery of immunological reactivity following x-irradiation. Exp Cell Res. 1962 Nov;28:455–457. doi: 10.1016/0014-4827(62)90305-1. [DOI] [PubMed] [Google Scholar]
  9. Hamaoka T., Katz D. H., Benacerraf B. Radioresistance of carrier-specific helper thymus-derived lymphocytes in mice. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3453–3458. doi: 10.1073/pnas.69.11.3453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ito T., Kino T., Cudkowicz G. B-ccell differentiation in the splenic environment: acquired susceptibility to the helper function of irradiated T cells. J Immunol. 1973 Feb;110(2):596–599. [PubMed] [Google Scholar]
  11. KENNEDY J. C., TILL J. E., SIMINOVITCH L., MCCULLOCH E. A. RADIOSENSITIVITY OF THE IMMUNE RESPONSE TO SHEEP RED CELLS IN THE MOUSE, AS MEASURED BY THE HEMOLYTIC PLAQUE METHOD. J Immunol. 1965 May;94:715–722. [PubMed] [Google Scholar]
  12. Kettman J., Dutton R. W. Radioresistance of the enhancing effect of cells from carrier-immunized mice in an in vitro primary immune response. Proc Natl Acad Sci U S A. 1971 Apr;68(4):699–703. doi: 10.1073/pnas.68.4.699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lafleur L., Miller R. G., Phillips R. A. A quantitative assay for the progenitors of bone marrow-associated lymphocytes. J Exp Med. 1972 Jun 1;135(6):1363–1374. doi: 10.1084/jem.135.6.1363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. MAKINODAN T., KASTENBAUM M. A., PETERSON W. J. Radiosensitivity of spleen cells from normal and preimmunized mice and its significance to intact animals. J Immunol. 1962 Jan;88:31–37. [PubMed] [Google Scholar]
  15. MILLER J. F., LEUCHARS E., CROSS A. M., DUKOR P. IMMUNOLOGIC ROLE OF THE THYMUS IN RADIATION CHIMERAS. Ann N Y Acad Sci. 1964 Nov 30;120:205–217. doi: 10.1111/j.1749-6632.1964.tb34718.x. [DOI] [PubMed] [Google Scholar]
  16. MacDonald H. R., Phillips R. A., Miller R. G. Allograft immunity in the mouse. II. Physical studies of the development of cytotoxic effector cells from their immediate progenitors. J Immunol. 1973 Aug;111(2):575–589. [PubMed] [Google Scholar]
  17. Miller J. F., Mitchell G. F. Cell to cell interaction in the immune response. I. Hemolysin-forming cells in neonatally thymectomized mice reconstituted with thymus or thoracic duct lymphocytes. J Exp Med. 1968 Oct 1;128(4):801–820. doi: 10.1084/jem.128.4.801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Miller J. F., Mitchell G. F. Thymus and antigen-reactive cells. Transplant Rev. 1969;1:3–42. doi: 10.1111/j.1600-065x.1969.tb00135.x. [DOI] [PubMed] [Google Scholar]
  19. Miller J. F., Osoba D. Current concepts of the immunological function of the thymus. Physiol Rev. 1967 Jul;47(3):437–520. doi: 10.1152/physrev.1967.47.3.437. [DOI] [PubMed] [Google Scholar]
  20. Nossal G. J., Pike B. L. Studies on the differentiation of B lymphocytes in the mouse. Immunology. 1973 Jul;25(1):33–45. [PMC free article] [PubMed] [Google Scholar]
  21. Osoba D. Some physical and radiobiological properties of immunologically reactive mouse spleen cells. J Exp Med. 1970 Aug 1;132(2):368–383. doi: 10.1084/jem.132.2.368. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Osoba D. Thymic control of cellular differentiation in the immunological system. Proc Soc Exp Biol Med. 1968 Feb;127(2):418–420. doi: 10.3181/00379727-127-32705. [DOI] [PubMed] [Google Scholar]
  23. Raff M. C. T and B lymphocytes in mice studied by using antisera against surface antigenic markers. Am J Pathol. 1971 Nov;65(2):467–478. [PMC free article] [PubMed] [Google Scholar]
  24. Raff M. C. Two distinct populations of peripheral lymphocytes in mice distinguishable by immunofluorescence. Immunology. 1970 Oct;19(4):637–650. [PMC free article] [PubMed] [Google Scholar]
  25. SCHREK R. Qualitative and quantitative reactions of lymphocytes to x rays. Ann N Y Acad Sci. 1961 Nov 13;95:839–848. doi: 10.1111/j.1749-6632.1961.tb50080.x. [DOI] [PubMed] [Google Scholar]
  26. Sado T., Kurotsu T., Kamisaku H. Further studies on the radioresistance of antibody producing cells: characterization of the survival curve. Radiat Res. 1971 Oct;48(1):179–188. [PubMed] [Google Scholar]
  27. Sato C., Sakka M. Cell population analysis of radiation damage and recovery of thymus and lymph nodes in mice. Radiat Res. 1969 Apr;38(1):204–213. [PubMed] [Google Scholar]
  28. el-Arini M. O., Osoba D. Differentiation of thymus-derived cells from precursors in mouse bone marrow. J Exp Med. 1973 Mar 1;137(3):821–837. doi: 10.1084/jem.137.3.821. [DOI] [PMC free article] [PubMed] [Google Scholar]

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