Skip to main content
Immunology logoLink to Immunology
. 1975 Jul;29(1):1–15.

The adjuvant activity of a non-toxic, water-soluble glycopeptide present in large quantities in the culture filtrate of Mycobacterium tuberculosis strain DT.

D E Stewart-Tull, T Shimono, S Kotani, M Kato, Y Ogawa, Y Yamamura, T Koga, C M Pearson
PMCID: PMC1445873  PMID: 806515

Abstract

A water-soluble mycobacterial glycopeptide was obtained in large quantities from the culture supernatant fluid of M. tuberculosis strain DT. This glycopeptide was strongly adjuvant-active when injected, in a water-in-oil emulsion contianing ovalbumin, into guinea-pigs. In addition, it was devoid of cord factor toxicity in mice, polyarthritogenic activity in rats and cavity stimulating activity in rabbit lungs.

Full text

PDF
1

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ASSELINEAU J., BLOCH H., LEDERER E. A toxic lipid component of the tubercle bacillus (cord factor). III. Occurrence and distribution in various bacterial extracts. Am Rev Tuberc. 1953 Jun;67(6):853–858. doi: 10.1164/art.1953.67.6.853. [DOI] [PubMed] [Google Scholar]
  2. Adam A., Ciorbaru R., Petit J. F., Lederer E. Isolation and properties of a macromolecular, water-soluble, immuno-adjuvant fraction from the cell wall of Mycobacterium smegmatis. Proc Natl Acad Sci U S A. 1972 Apr;69(4):851–854. doi: 10.1073/pnas.69.4.851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Azuma I., Kanetsuna F., Kada Y., Takashima T., Yamamura Y. Adjuvant-polyarthritogenicity of cell walls of mycobacteria, nocardia and corynebacteria. Jpn J Microbiol. 1972 Jul;16(4):333–336. doi: 10.1111/j.1348-0421.1972.tb00666.x. [DOI] [PubMed] [Google Scholar]
  4. Braun V., Rehn K. Chemical characterization, spatial distribution and function of a lipoprotein (murein-lipoprotein) of the E. coli cell wall. The specific effect of trypsin on the membrane structure. Eur J Biochem. 1969 Oct;10(3):426–438. doi: 10.1111/j.1432-1033.1969.tb00707.x. [DOI] [PubMed] [Google Scholar]
  5. Braun V., Sieglin U. The covalent murein-lipoprotein structure of the Escherichia coli cell wall. The attachment site of the lipoprotein on the murein. Eur J Biochem. 1970 Apr;13(2):336–346. doi: 10.1111/j.1432-1033.1970.tb00936.x. [DOI] [PubMed] [Google Scholar]
  6. Braun V., Wolff H. The murein-lipoprotein linkage in the cell wall of Escherichia coli. Eur J Biochem. 1970 Jun;14(2):387–391. doi: 10.1111/j.1432-1033.1970.tb00301.x. [DOI] [PubMed] [Google Scholar]
  7. Chedid L., Parant M., Parant F., Gustafson R. H., Berger F. M. Biological study of a nontoxic, water-soluble immunoadjuvant from mycobacterial cell walls. Proc Natl Acad Sci U S A. 1972 Apr;69(4):855–858. doi: 10.1073/pnas.69.4.855. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Davies M., Stewart-Tull D. E. The dual affinity of a mycobacterial glycopeptide for sheep erythrocyte membranes and guinea-pig gamma-globulin. Immunology. 1973 Jul;25(1):1–9. [PMC free article] [PubMed] [Google Scholar]
  9. KOTANI S., HASHIMOTO S., MATSUBARA T., KATO K., HARADA K., KOGAMI J. LYSIS OF ISOLATED BCG CELL WALLS WITH ENZYMES. 2. DEMONSTRATION OF 'BOUND WAX D' AS A COMPONENT OF BCG CELL WALLS. Biken J. 1963 Oct;6:181–196. [PubMed] [Google Scholar]
  10. Migliore-Samour D., Jollès P. A hydrosoluble, adjuvant-active mycobacterial "polysaccharide-peptidoglycan". Preparation by a simple extraction technique of the bacterial cells (strain Peurois). FEBS Lett. 1972 Sep 15;25(2):301–304. doi: 10.1016/0014-5793(72)80509-x. [DOI] [PubMed] [Google Scholar]
  11. SEIBERT F. B., FIGUEROA E. S., DUFOUR E. H. Isolation, identification, and classification of proteins of tuberculin and the tubercle bacillus. Am Rev Tuberc. 1955 May;71(5):704–721. doi: 10.1164/artpd.1955.71.5.704. [DOI] [PubMed] [Google Scholar]
  12. STEWART-TULL D. E., WHITE R. G. THE OCCURRENCE OF MURAMIC ACID IN WAX D PREPARATIONS OF MYCOBACTERIA. J Gen Microbiol. 1964 Jan;34:43–49. doi: 10.1099/00221287-34-1-43. [DOI] [PubMed] [Google Scholar]
  13. Stewart-Tull D. E., Davies M. Adjuvant activity of Mycobacterium leprae. Infect Immun. 1972 Dec;6(6):909–912. doi: 10.1128/iai.6.6.909-912.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Stewart-Tull D. E., White R. G. The influence of age of culture on the production of adjuvant-active peptidoglycolipids by saprophytic mycobacteria. Immunology. 1967 Mar;12(3):349–359. [PMC free article] [PubMed] [Google Scholar]
  15. Stewart-Tull D. E., Wilkinson P. C. The affinity of mycobacterial glycopetides for guinea-pig gamma immunoglobulin and its fragments. Immunology. 1973 Aug;25(2):205–216. [PMC free article] [PubMed] [Google Scholar]
  16. Stewart-Tull D. E., Wilkinson P. C., White R. G. The affinity of a mycobacterial glycopeptide for guinea-pig gamma-globulin. Immunology. 1965 Aug;9(2):151–160. [PMC free article] [PubMed] [Google Scholar]
  17. TAKEYA K., HISATSUNE K., NAKASHIMA K. A cell-wall mucopeptide complex obtained from the culture filtrate of tubercle bacilli. Biochim Biophys Acta. 1961 Dec 23;54:595–597. doi: 10.1016/0006-3002(61)90107-x. [DOI] [PubMed] [Google Scholar]
  18. WAKSMAN B. H., PEARSON C. M., SHARP J. T. Studies of arthritis and other lesions induced in rats by injection of mycobacterial adjuvant. II. Evidence that the disease is a disseminated immunologic response to exogenous antigen. J Immunol. 1960 Oct;85:403–417. [PubMed] [Google Scholar]
  19. WHITE R. G., BERNSTOCK L., JOHNS R. G., LEDERER E. The influence of components of M. tuberculosis and other Mycobacteria upon antibody production to ovalbumin. Immunology. 1958 Jan;1(1):54–66. [PMC free article] [PubMed] [Google Scholar]
  20. WHITE R. G., COONS A. H., CONNOLLY J. M. Studies on antibody production. IV. The role of a wax fraction of Mycobacterium tuberculosis in adjuvant emulsions on the production of antibody to egg albumin. J Exp Med. 1955 Jul 1;102(1):83–104. doi: 10.1084/jem.102.1.83. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. WHITE R. G., JOLLES P., SAMOUR D., LEDERER E. CORRELATION OF ADJUVANT ACTIVITY AND CHEMICAL STRUCTURE OF WAX D FRACTIONS OF MYCOBACTERIA. Immunology. 1964 Mar;7:158–171. [PMC free article] [PubMed] [Google Scholar]
  22. Wood F. D., Pearson C. M., Tanaka A. Capacity of mycobacterial wax D and its subfractions to induce adjuvant arthritis in rats. Int Arch Allergy Appl Immunol. 1969;35(5):456–467. doi: 10.1159/000230198. [DOI] [PubMed] [Google Scholar]
  23. YAMAMURA Y. The pathogenesis of tuberculous cavities. Bibl Tuberc. 1958;13:13–37. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES